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Literature summary extracted from

  • Vander Jagt, D.L.
    2-Ketoaldehyde dehydrogenase from rat liver (1982), Methods Enzymol., 89, 513-515.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.49 L-2-Aminopropan-1-ol activation of aldehyde oxidation Rattus norvegicus
1.2.1.49 L-serine methyl ester activation of aldehyde oxidation Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.49 methylglyoxal + NADP+ + H2O Rattus norvegicus
-
 pyruvate + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.49 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.49 using ammonium sulfate fractionation and chromatography on DEAE-Sephadex A-50 column, Blue-dextran affinity column and CM-Sephadex C-50 column Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.49 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.49 7.25
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
1.2.1.49 -20°C, 50% glycerol Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.49 methylglyoxal + NADP+ + H2O
-
 Rattus norvegicus pyruvate + NADPH + H+
-
 ?
1.2.1.49 additional information L-serine methyl ester or L-2-aminopropan-1-ol are necessary to activate the enzyme Rattus norvegicus ?
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.49 NADP+
-
 Rattus norvegicus