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Literature summary extracted from

  • Ando, M.; Yoshimoto, T.; Ogushi, S.; Rikitake, K.; Shibata, S.; Tsuru, D.
    Formaldehyde dehydrogenase from Pseudomonas putida. Purification and some properties (1979), J. Biochem., 85, 1165-1172.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.46 5,5'-dithiobis(2-nitrobenzoate) partial Pseudomonas putida
1.2.1.46 Cd2+
-
 Pseudomonas putida
1.2.1.46 diisopropylphosphofluoridate partial Pseudomonas putida
1.2.1.46 EDTA partial Pseudomonas putida
1.2.1.46 Hg2+
-
 Pseudomonas putida
1.2.1.46 Mn2+ partial Pseudomonas putida
1.2.1.46 N-Acetylimidazole partial Pseudomonas putida
1.2.1.46 Ni2+
-
 Pseudomonas putida
1.2.1.46 o-phenanthroline partial Pseudomonas putida
1.2.1.46 p-chloromercuribenzoate
-
 Pseudomonas putida
1.2.1.46 phenylmethanesulfonyl fluoride
-
 Pseudomonas putida
1.2.1.46 Sodium tetrathionate partial Pseudomonas putida
1.2.1.46 Zn2+ partial Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.46 0.056
-
 NAD+
-
 Pseudomonas putida
1.2.1.46 0.067
-
 formaldehyde
-
 Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.46 75000
-
 2 * 75000, SDS-PAGE Pseudomonas putida
1.2.1.46 150000
-
 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.46 formaldehyde + NAD+ + H2O Pseudomonas putida enzyme of creatinine metabolism formate + NADH
-
 ?
1.2.1.46 formaldehyde + NAD+ + H2O Pseudomonas putida C-83 enzyme of creatinine metabolism formate + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.46 Pseudomonas putida
-
 C-83
-
1.2.1.46 Pseudomonas putida C-83
-
 C-83
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.46 C-83 Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.46 additional information
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.46 formaldehyde + NAD+ + H2O
-
 Pseudomonas putida formate + NADH
-
 ir
1.2.1.46 formaldehyde + NAD+ + H2O enzyme of creatinine metabolism Pseudomonas putida formate + NADH
-
 ?
1.2.1.46 formaldehyde + NAD+ + H2O
-
 Pseudomonas putida C-83 formate + NADH
-
 ir
1.2.1.46 formaldehyde + NAD+ + H2O enzyme of creatinine metabolism Pseudomonas putida C-83 formate + NADH
-
 ?
1.2.1.46 glyoxal + NAD+ + H2O
-
 Pseudomonas putida glyoxylate + NADH
-
 ?
1.2.1.46 glyoxal + NAD+ + H2O
-
 Pseudomonas putida C-83 glyoxylate + NADH
-
 ?
1.2.1.46 propionaldehyde + NAD+ + H2O
-
 Pseudomonas putida propionate + NADH
-
 ?
1.2.1.46 propionaldehyde + NAD+ + H2O
-
 Pseudomonas putida C-83 propionate + NADH
-
 ?
1.2.1.46 pyruvaldehyde + NAD+ + H2O
-
 Pseudomonas putida pyruvate + NADH
-
 ?
1.2.1.46 pyruvaldehyde + NAD+ + H2O
-
 Pseudomonas putida C-83 pyruvate + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.46 dimer 2 * 75000, SDS-PAGE Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.46 40
-
-
 Pseudomonas putida

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.46 40
-
 30 min, pH 7.5, stable Pseudomonas putida
1.2.1.46 55
-
 pH 7.5, 30 min, 50% loss of activity Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.46 7.8
-
 formaldehyde Pseudomonas putida

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.46 6.6 8.3 at pH 6.6 and 8.3 about 50% of activity maximum Pseudomonas putida

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.2.1.46 8
-
 15 h, 20% loss of activity Pseudomonas putida
1.2.1.46 8.5 10 stable Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.46 NAD+
-
 Pseudomonas putida