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Literature summary extracted from

  • Maruyama, K.
    Interaction of 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase with Reactive Blue 2 and related dyes (1988), J. Biochem., 103, 714-721.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.312 Lubrol the enzyme activity increases in a sigmoidal manner with increasing concentrations, minimum concentration necessary for the increase of enzyme activity is higher than 0.05 mM, effectively eliminates the dye inhibition Pseudomonas straminea

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.312 2-pyrone-4,6-dicarboxylic acid non-competitive inhibition with respect to 4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde Pseudomonas straminea
1.1.1.312 Blue dextran competitive inhibitor with respect to NADP+ Pseudomonas straminea
1.1.1.312 methoxy Reactive Blue 2 inhibits the enzyme non-competitively with respect to 4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde and competitively with respect to NADP+ Pseudomonas straminea
1.1.1.312 NADH competitive inhibition with respect to NAD+, non-competitive inhibition with respect to 4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde Pseudomonas straminea
1.1.1.312 NADPH competitive inhibition with respect to NADP, non-competitive inhibition with respect to 4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde Pseudomonas straminea
1.1.1.312 Reactive blue 2 inhibits the enzyme non-competitively with respect to 4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde and competitively with respect to NADP+ Pseudomonas straminea
1.1.1.312 Reactive Blue 4 inhibits the enzyme non-competitively with respect to 4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde and competitively with respect to NADP+ Pseudomonas straminea

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.312 Pseudomonas straminea
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.312 4-carboxy-2-hydroxymuconate semialdehyde hemiacetal + NADP+ = 2-oxo-2H-pyran-4,6-dicarboxylate + NADPH + H+ ordered bi-bi mechanism Pseudomonas straminea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.312 4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde + NADP+
-
Pseudomonas straminea 4-carboxy-2-hydroxy-cis,cis-muconate + NADPH following intramolecular dehydrogenation to the lactone 2-pyrone-4,6-dicarboxylic acid ?
1.1.1.312 additional information interaction with Blue Dextran-2000 and related dyes Pseudomonas straminea ?
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.312 dimer
-
Pseudomonas straminea

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.312 NADP+
-
Pseudomonas straminea

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.312 0.00006
-
Blue dextran
-
Pseudomonas straminea
1.1.1.312 0.0002
-
Reactive blue 2 coenzyme NADP+, similar value with coenzyme NAD+ Pseudomonas straminea
1.1.1.312 0.0002
-
methoxy Reactive Blue 2 coenzyme NADP+, similar value with coenzyme NAD+ Pseudomonas straminea
1.1.1.312 0.0045
-
Reactive Blue 4 coenzyme NADP+, similar value with coenzyme NAD+ Pseudomonas straminea
1.1.1.312 0.072
-
Reactive blue 2
-
Pseudomonas straminea