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Literature summary extracted from

  • Gross, G.G.; Kreiten, W.
    Reduction of coenzyme A thioesters of cinnamic acids with an enzyme preparation from lignifying tissue of Forsythia (1975), FEBS Lett., 54, 259-262.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.44 0.022
-
 feruloyl-CoA
-
 Forsythia suspensa
1.2.1.44 0.045
-
 NADPH cosubstrate feruloyl-CoA Forsythia suspensa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.44 additional information addition of Mg2+, Mn2+, Zn2+, Fe2+ has no effect Forsythia suspensa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.44 40000
-
 gel filtration Forsythia suspensa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.44 feruloyl-CoA + NADPH + H+ Forsythia suspensa enzyme of lignification ferulic aldehyde + CoA + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.44 Forsythia suspensa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.44 partial Forsythia suspensa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.44 stem
-
 Forsythia suspensa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.44 5-hydroxyferuloyl-CoA + NADPH + H+ low activity Forsythia suspensa 5-hydroxyferulic aldehyde + CoA + NADP+
-
 ?
1.2.1.44 5-hydroxyferuloyl-CoA + NADPH + H+ i.e. 4,5-dihydroxy-3-methoxycinnamoyl-CoA Forsythia suspensa 5-hydroxyferulic aldehyde + CoA + NADP+
-
 ?
1.2.1.44 caffeoyl-CoA + NADPH poor substrate Forsythia suspensa caffeic aldehyde + CoA + NADP+
-
 ?
1.2.1.44 cinnamoyl-CoA + NADPH low activity Forsythia suspensa cinnamaldehyde + CoA + NADP+
-
 ?
1.2.1.44 feruloyl-CoA + NADPH
-
 Forsythia suspensa ferulic aldehyde + CoA + NADP+
-
 ?
1.2.1.44 feruloyl-CoA + NADPH + H+ enzyme of lignification Forsythia suspensa ferulic aldehyde + CoA + NADP+
-
 ?
1.2.1.44 p-coumaroyl-CoA + NADPH i.e. p-hydroxycinnamoyl-CoA, poor substrate Forsythia suspensa p-coumaric aldehyde + CoA + NADP+
-
 ?
1.2.1.44 sinapoyl-CoA + NADPH low activity Forsythia suspensa sinapic aldehyde + CoA + NADP+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.44 7.4 7.8
-
 Forsythia suspensa

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.44 NADP+ not: NADH Forsythia suspensa