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Literature summary extracted from

  • Lee, C.Y.; Meighen, E.A.
    Cysteine-286 as the site of acylation of the lux-specific fatty acyl-CoA reductase (1997), Biochim. Biophys. Acta, 1338, 215-222.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.42 expression in Escherichia coli Photobacterium phosphoreum
1.2.1.42 expression in Escherichia coli Photobacterium leiognathi
1.2.1.50 expression in Escherichia coli Photobacterium phosphoreum
1.2.1.50 expression in Escherichia coli Photobacterium leiognathi

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.42 C171S 77% of wild-type activity Photobacterium phosphoreum
1.2.1.42 C171S 99% of wild-type activity Photobacterium leiognathi
1.2.1.42 C279S 90% of wild-type activity Photobacterium phosphoreum
1.2.1.42 C279S 79% of wild -type activity Photobacterium leiognathi
1.2.1.42 C286S 9% of wild-type activity Photobacterium phosphoreum
1.2.1.42 C286S 2% of wild-type activity Photobacterium leiognathi
1.2.1.50 C171S acylation and specific activity similar to the wild-type Photobacterium phosphoreum
1.2.1.50 C171S acylation and specific activity similar to the wild-type Photobacterium leiognathi
1.2.1.50 C279S acylation and specific activity similar to the wild-type Photobacterium phosphoreum
1.2.1.50 C279S acylation and specific activity similar to the wild-type Photobacterium leiognathi
1.2.1.50 C286S no acylation and no activity Photobacterium phosphoreum
1.2.1.50 C286S no acylation and no activity Photobacterium leiognathi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.50 tetradecanoyl-CoA + NADPH + H+ Photobacterium phosphoreum
-
 tetradecanal + CoA + NADP+
-
 ?
1.2.1.50 tetradecanoyl-CoA + NADPH + H+ Photobacterium leiognathi
-
 tetradecanal + CoA + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.42 Photobacterium leiognathi
-
-
-
1.2.1.42 Photobacterium phosphoreum
-
-
-
1.2.1.50 Photobacterium leiognathi
-
-
-
1.2.1.50 Photobacterium phosphoreum
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.42 additional information
-
 20.0 light emission units/mg after 4 min incubation in luciferase coupled assay Photobacterium leiognathi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.42 hexadecanoyl-CoA + NAD(P)H
-
 Photobacterium phosphoreum hexadecanal + NAD(P)+
-
 ?
1.2.1.42 hexadecanoyl-CoA + NAD(P)H
-
 Photobacterium leiognathi hexadecanal + NAD(P)+
-
 ?
1.2.1.50 tetradecanoyl-CoA + NADPH + H+
-
 Photobacterium phosphoreum tetradecanal + CoA + NADP+
-
 ?
1.2.1.50 tetradecanoyl-CoA + NADPH + H+
-
 Photobacterium leiognathi tetradecanal + CoA + NADP+
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.42 NAD(P)H
-
 Photobacterium phosphoreum
1.2.1.42 NAD(P)H
-
 Photobacterium leiognathi