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Literature summary extracted from

  • Wang, X.; Kolattukudy, P.E.
    Solubilization, purification and characterization of fatty acyl-CoA reductase from duck uropygial gland (1995), Biochem. Biophys. Res. Commun., 208, 210-215.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.42 bovine serum albumin 2 mg/ml, 5fold increase in enzyme activity Anas platyrhynchos
1.2.1.42 Lipids 150% increase in activity Anas platyrhynchos
1.2.1.50 bovine serum albumin 2 mg per ml increases activity by more than 5 times Anas platyrhynchos

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.42 0.029
-
 hexadecanoyl-CoA
-
 Anas platyrhynchos
1.2.1.42 0.067
-
 NADPH
-
 Anas platyrhynchos
1.2.1.50 0.029
-
 palmitoyl-CoA
-
 Anas platyrhynchos
1.2.1.50 0.067
-
 NADPH
-
 Anas platyrhynchos

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.42 membrane
-
 Anas platyrhynchos 16020
-
1.2.1.50 microsome membrane-bound enzyme Anas platyrhynchos
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.42 56000
-
 x * 56000, SDS-PAGE Anas platyrhynchos
1.2.1.50 56000
-
 x * 56000, SDS-PAGE Anas platyrhynchos

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.42 Anas platyrhynchos
-
-
-
1.2.1.50 Anas platyrhynchos
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.42 solubilization from microsomal preparation with 20% glycerol and 3 M NaCl, affinity chromatography Anas platyrhynchos
1.2.1.50
-
 Anas platyrhynchos

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.42 uropygial gland
-
 Anas platyrhynchos
-
1.2.1.50 uropygial gland
-
 Anas platyrhynchos
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.42 0.033
-
-
 Anas platyrhynchos
1.2.1.50 33
-
-
 Anas platyrhynchos

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.42 hexadecanoyl-CoA + NAD(P)H NADH gives almost 60% of the activity obtained with NADPH, after palmitoyl-CoA affinity column purification enzyme is absolutely dependent on NADPH as cofactor Anas platyrhynchos hexadecanal + NAD(P)+
-
 ?
1.2.1.50 palmitoyl-CoA + NADPH + H+
-
 Anas platyrhynchos hexadecanal + CoA + NADP+
-
 r

Subunits

EC Number Subunits Comment Organism
1.2.1.42 ? x * 56000, SDS-PAGE Anas platyrhynchos
1.2.1.50 monomer x * 56000, SDS-PAGE Anas platyrhynchos

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.42 NAD(P)H after palmitoyl-CoA affinity column purification enzyme is absolutely dependent on NADPH as cofactor Anas platyrhynchos