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Literature summary extracted from

  • Gross, G.G.; Bolkart, K.H.; Zenk, M.H.
    Reduction of cinnamic acid to cinnamaldehyde and alcohol (1968), Biochem. Biophys. Res. Commun., 32, 173-178.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.30 120000
-
gel filtration Neurospora crassa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.30 aromatic acids + NADPH + H+ + ATP Neurospora crassa
-
aromatic aldehydes + NADP+ + AMP + diphosphate + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.30 Neurospora crassa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.30 by precipitation of nucleic acids with protamine sulfate, ammonium sulphate fractionation, adsorption on calcium-phosphate gel and chromatography on TEAE-cellulose and on hydroxylapatite Neurospora crassa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.30 mycelium
-
Neurospora crassa
-

Storage Stability

EC Number Storage Stability Organism
1.2.1.30 0°C, in the presence of dithiothreitol, 2 months Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.30 aromatic acids + NADPH + H+ + ATP
-
Neurospora crassa aromatic aldehydes + NADP+ + AMP + diphosphate + H2O
-
?
1.2.1.30 aromatic acids + NADPH + H+ + ATP
-
Neurospora crassa aromatic aldehydes + NADP+ + AMP + diphosphate + H2O
-
ir
1.2.1.30 caffeic acid + NADPH + ATP
-
Neurospora crassa 3-(3,4-dihydroxyphenyl)-2-propen-1-al + NADP+ + AMP + H2O
-
?
1.2.1.30 cinnamic acid + NADPH + ATP
-
Neurospora crassa 3-phenyl-2-propen-1-al + NADP+ + AMP + H2O
-
?
1.2.1.30 ferulic acid + NADPH + H+ + ATP
-
Neurospora crassa 3-(4-hydroxy-3-methoxyphenyl)-2-propen-1-al + NADP+ + AMP + diphosphate
-
?
1.2.1.30 m-coumaric acid + NADPH + ATP
-
Neurospora crassa 3-(3-hydroxyphenyl)-2-propen-1-al + NADP+ + AMP + H2O
-
?
1.2.1.30 o-coumaric acid + NADPH + ATP
-
Neurospora crassa 3-(2-hydroxyphenyl)-2-propen-1-al + NADP+ + AMP + H2O
-
?
1.2.1.30 p-coumaric acid + NADPH + ATP
-
Neurospora crassa 3-(4-hydroxphenyl)-2-propen-1-al + NADP+ + AMP + H2O
-
?
1.2.1.30 sinapic acid + NADPH + ATP
-
Neurospora crassa 3-(4-hydroxy-3,5-dimethoxyphenyl)-2-propen-1-al + NADP+ + AMP + H2O
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.30 35 36 salicylate + ATP + NADPH Neurospora crassa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.30 7.7 8.1 salicylate + ATP + NADPH Neurospora crassa
1.2.1.30 8
-
-
Neurospora crassa

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.30 ATP completely dependent on Neurospora crassa
1.2.1.30 NADPH completely dependent on Neurospora crassa