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Literature summary extracted from

  • Friedebold, J.; Bowien, B.
    Physiological and biochemical characterization of the soluble formate dehydrogenase, a molybdoenzyme from Alcaligenes eutrophus (1993), J. Bacteriol., 175, 4719-4728.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.17.1.9 N3- and NO3- stabilize Cupriavidus necator

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.1.9 CN-
-
 Cupriavidus necator
1.17.1.9 Hg2+
-
 Cupriavidus necator
1.17.1.9 N3-
-
 Cupriavidus necator
1.17.1.9 NO3- 10 mM KNO3, 38% inhibition Cupriavidus necator

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.1.9 0.09
-
 NAD+
-
 Cupriavidus necator
1.17.1.9 3.3
-
 formate
-
 Cupriavidus necator

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.1.9 Molybdenum 0.64 gatom per mol of enzyme. The enzyme contains molybdopterin guanine dinucleotide Cupriavidus necator

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.1.9 11600
-
 alpha,beta,gamma,delta, 1 * 110000 + 1 * 57000 + 1 * 19400 + 1 * 11600, SDS-PAGE Cupriavidus necator
1.17.1.9 19400
-
 alpha,beta,gamma,delta, 1 * 110000 + 1 * 57000 + 1 * 19400 + 1 * 11600, SDS-PAGE Cupriavidus necator
1.17.1.9 57000
-
 alpha,beta,gamma,delta, 1 * 110000 + 1 * 57000 + 1 * 19400 + 1 * 11600, SDS-PAGE Cupriavidus necator
1.17.1.9 110000
-
 alpha,beta,gamma,delta, 1 * 110000 + 1 * 57000 + 1 * 19400 + 1 * 11600, SDS-PAGE Cupriavidus necator
1.17.1.9 197000
-
 non-denaturing gradient PAGE Cupriavidus necator

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.9 Cupriavidus necator
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.1.9 additional information
-
-
 Cupriavidus necator

Storage Stability

EC Number Storage Stability Organism
1.17.1.9 -20°C, stable for several weeks Cupriavidus necator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.9 formate + 2,6-dichloroindophenol 54% of the activity with NAD+ Cupriavidus necator CO2 + reduced 2,6-dichlorophenolindophenol
-
 ?
1.17.1.9 formate + benzyl viologen 113% of the activity with NAD+ Cupriavidus necator CO2 + reduced benzyl viologen
-
 ?
1.17.1.9 formate + ferricyanide 10% of the activity with NAD+ Cupriavidus necator CO2 + ferrocyanide
-
 ?
1.17.1.9 formate + methyl viologen 74% of the activity with NAD+ Cupriavidus necator CO2 + reduced methyl viologen
-
 ?
1.17.1.9 formate + methylene blue 119% of the activity with NAD+ Cupriavidus necator CO2 + reduced methylene blue
-
 ?
1.17.1.9 formate + NAD+
-
 Cupriavidus necator CO2 + NADH + H+
-
 ?
1.17.1.9 formate + nitrobluetetrazolium 54% of the activity with NAD+ Cupriavidus necator CO2 + reduced nitrobluetetrazolium
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.1.9 tetramer alpha,beta,gamma,delta, 1 * 110000 + 1 * 57000 + 1 * 19400 + 1 * 11600, SDS-PAGE Cupriavidus necator

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.17.1.9 45
-
 rapid inactivation above Cupriavidus necator

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.1.9 7.7
-
 phosphate buffer Cupriavidus necator
1.17.1.9 7.9
-
 Tris-HCl buffer Cupriavidus necator

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.17.1.9 5 10 completely inactive below pH 5.5 and above pH 10.0 Cupriavidus necator

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.17.1.9 7.5
-
 optimal stability in presence of 10 mM KNO3 Cupriavidus necator
1.17.1.9 8
-
 rapid decrease of stability above Cupriavidus necator

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.9 FMN 0.9 mol of FMN per mol of enzyme Cupriavidus necator
1.17.1.9 NAD+
-
 Cupriavidus necator