BRENDA - Enzyme Database

S-Formylgluthathione: the substrate for formate dehydrogenase in methanol-utilizing yeasts

Van Dijken, J.P.; Oostra-Demkes, G.J.; Otto, R.; Harder, W.; Arch. Microbiol. 111, 77-83 (1976)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.17.1.9
0.07
-
NAD+
-
Ogataea angusta
1.17.1.9
1.1
-
S-formylglutathione
-
Ogataea angusta
1.17.1.9
40
-
formate
-
Ogataea angusta
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.17.1.9
additional information
Ogataea angusta
S-formylglutathione rather than free formate is an intermediate in oxidation of methanol by yeast
?
-
-
-
1.17.1.9
additional information
Ogataea pini
S-formylglutathione rather than free formate is an intermediate in oxidation of methanol by yeast
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.17.1.9
Candida boidinii
-
-
-
1.17.1.9
Ogataea angusta
-
-
-
1.17.1.9
Ogataea pini
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.17.1.9
-
Ogataea angusta
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.17.1.9
2.83
-
-
Ogataea angusta
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.17.1.9
formate + NAD+
-
288108
Candida boidinii
CO2 + NADH
-
-
-
?
1.17.1.9
formate + NAD+
-
288108
Ogataea angusta
CO2 + NADH
-
-
-
?
1.17.1.9
formate + NAD+
-
288108
Ogataea pini
CO2 + NADH
-
-
-
?
1.17.1.9
additional information
S-formylglutathione rather than free formate is an intermediate in oxidation of methanol by yeast
288108
Ogataea angusta
?
-
-
-
-
1.17.1.9
additional information
S-formylglutathione rather than free formate is an intermediate in oxidation of methanol by yeast
288108
Ogataea pini
?
-
-
-
-
1.17.1.9
S-formylglutathione + NAD+
about 40fold lower Km-value than with formate
288108
Ogataea angusta
?
-
-
-
?
Temperature Optimum [░C]
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
1.17.1.9
60
-
-
Ogataea angusta
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.17.1.9
7
-
-
Ogataea angusta
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.17.1.9
NAD+
-
Ogataea pini
1.17.1.9
NAD+
-
Ogataea angusta
1.17.1.9
NAD+
-
Candida boidinii
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.17.1.9
NAD+
-
Ogataea pini
1.17.1.9
NAD+
-
Ogataea angusta
1.17.1.9
NAD+
-
Candida boidinii
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.17.1.9
0.07
-
NAD+
-
Ogataea angusta
1.17.1.9
1.1
-
S-formylglutathione
-
Ogataea angusta
1.17.1.9
40
-
formate
-
Ogataea angusta
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.17.1.9
additional information
Ogataea angusta
S-formylglutathione rather than free formate is an intermediate in oxidation of methanol by yeast
?
-
-
-
1.17.1.9
additional information
Ogataea pini
S-formylglutathione rather than free formate is an intermediate in oxidation of methanol by yeast
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.17.1.9
-
Ogataea angusta
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.17.1.9
2.83
-
-
Ogataea angusta
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.17.1.9
formate + NAD+
-
288108
Candida boidinii
CO2 + NADH
-
-
-
?
1.17.1.9
formate + NAD+
-
288108
Ogataea angusta
CO2 + NADH
-
-
-
?
1.17.1.9
formate + NAD+
-
288108
Ogataea pini
CO2 + NADH
-
-
-
?
1.17.1.9
additional information
S-formylglutathione rather than free formate is an intermediate in oxidation of methanol by yeast
288108
Ogataea angusta
?
-
-
-
-
1.17.1.9
additional information
S-formylglutathione rather than free formate is an intermediate in oxidation of methanol by yeast
288108
Ogataea pini
?
-
-
-
-
1.17.1.9
S-formylglutathione + NAD+
about 40fold lower Km-value than with formate
288108
Ogataea angusta
?
-
-
-
?
Temperature Optimum [░C] (protein specific)
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
1.17.1.9
60
-
-
Ogataea angusta
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.17.1.9
7
-
-
Ogataea angusta