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Literature summary extracted from

  • Izumi, Y.; Kanzaki, H.; Morita, S.; Futazuka, H.; Yamada, H.
    Characterization of crystalline formate dehydrogenase from Candida methanolica (1989), Eur. J. Biochem., 182, 333-341.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.1.9 benzaldehyde slight inhibition Candida methanolica
1.17.1.9 Cd2+ CdCl2 Candida methanolica
1.17.1.9 CN- NaCN Candida methanolica
1.17.1.9 Hg2+
-
 Candida methanolica
1.17.1.9 iodoacetamide
-
 Candida methanolica
1.17.1.9 N3-
-
 Candida methanolica
1.17.1.9 Ni2+ NiCl2 Candida methanolica
1.17.1.9 o-nitrobenzaldehyde slight inhibition Candida methanolica
1.17.1.9 PCMB
-
 Candida methanolica
1.17.1.9 pyridoxal 5'-phosphate slight inhibition Candida methanolica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.1.9 0.11
-
 NAD+
-
 Candida methanolica
1.17.1.9 3
-
 formate
-
 Candida methanolica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.1.9 43000
-
 2 * 43000, SDS-PAGE Candida methanolica
1.17.1.9 71000
-
 equilibrium sedimentation Candida methanolica
1.17.1.9 82000
-
 velocity sedimentation Candida methanolica
1.17.1.9 84000
-
 gel filtration Candida methanolica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.1.9 formate + NAD+ Candida methanolica one of the key enzymes in the assimilation of C1 compounds, such as methanol CO2 + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.9 Candida methanolica
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.17.1.9 formate + NAD+ = CO2 + NADH + H+ sequential ordered bi-bi mechanism Candida methanolica
a

Storage Stability

EC Number Storage Stability Organism
1.17.1.9 -20°C, potassium phosphate buffer, pH 7.0, 45% v/v glycerol, 1.0 mM dithiothreitol, stable for 2 months Candida methanolica
1.17.1.9 4°C, 0.01 M buffer, 10 days, 50% loss of activity Candida methanolica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.9 formate + NAD+
-
 Candida methanolica CO2 + NADH + H+
-
 ?
1.17.1.9 formate + NAD+ one of the key enzymes in the assimilation of C1 compounds, such as methanol Candida methanolica CO2 + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.1.9 dimer 2 * 43000, SDS-PAGE Candida methanolica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.17.1.9 50
-
-
 Candida methanolica

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.17.1.9 55
-
 10 min, pH 6.5-9.5, stable up to Candida methanolica
1.17.1.9 60
-
 10 min, 36% loss of activity Candida methanolica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.1.9 6.5 9.5
-
 Candida methanolica

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.17.1.9 5.5
-
 30°C, 10 min, 61% loss of activity Candida methanolica
1.17.1.9 6.5 9.5 10 min, 30°C, stable Candida methanolica
1.17.1.9 10.5
-
 30°C, 10 min, 41% loss of activity Candida methanolica

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.9 NAD+
-
 Candida methanolica