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Literature summary extracted from

  • Avilova, T.V.; Egorova, O.A.; Ioanesyan, L.S.; Egorov, A.M.
    Biosynthesis, isolation and properties of NAD-dependent formate dehydrogenase from the yeast Candida methylica (1985), Eur. J. Biochem., 152, 657-662.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.1.9 CN-
-
 [Candida] methylica
1.17.1.9 Dithionitrobenzoate
-
 [Candida] methylica
1.17.1.9 N3-
-
 [Candida] methylica
1.17.1.9 PCMB
-
 [Candida] methylica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.1.9 0.1
-
 NAD+
-
 [Candida] methylica
1.17.1.9 13
-
 formate
-
 [Candida] methylica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.1.9 46000
-
 2 * 46000, SDS-PAGE [Candida] methylica
1.17.1.9 70000
-
 gel filtration [Candida] methylica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.1.9 formate + NAD+ [Candida] methylica induced by methanol and formate CO2 + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.9 [Candida] methylica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.1.9 two-step procedure and single-step procedure [Candida] methylica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.1.9 16
-
-
 [Candida] methylica

Storage Stability

EC Number Storage Stability Organism
1.17.1.9 4°C, enzyme dissolved in glycerol, retains activity for a few months [Candida] methylica
1.17.1.9 4°C, pH 7.6, in presence of cofactor, EDTA, 2-mercaptoethanol and glycerol, 50% loss of activity after 72 h. NADH decrease the inactivation constant 2.5fold, formate decreases the inactivation constant 5fold, 2-mercaptoethanol decreases the inactivation constant 8fold [Candida] methylica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.9 formate + NAD+
-
 [Candida] methylica CO2 + NADH + H+
-
 ?
1.17.1.9 formate + NAD+ induced by methanol and formate [Candida] methylica CO2 + NADH + H+
-
 ?
1.17.1.9 formate + phenazine methosulfate no activity [Candida] methylica CO2 + reduced phenazine methosulfate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.1.9 dimer 2 * 46000, SDS-PAGE [Candida] methylica

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.9 NAD+
-
 [Candida] methylica