Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Callewaert, D.M.; Rosemblatt, M.S.; Tchen, T.T.
    Purification and properties of 4-aminobutanal dehydrogenase from a Pseudomonas species (1974), J. Biol. Chem., 249, 1737-1741.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.19 75000
-
 3 * 75000, SDS-PAGE Pseudomonas sp.
1.2.1.19 228000
-
 gel filtration, PAGE Pseudomonas sp.

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.2.1.19 Glycerol stable in 30% with 5 mM mercaptoethanol + 5 mM EDTA Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.19 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.19 from putrescine-grown cells, using column chromatography on DEAE-cellulose, DEAE-Sephadex A-50, Sephadex G-200, Hydroxylapatite and Sephadex G-200 in 150 mM NaCl Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.19 0.22
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.19 4-aminobutanal + NAD+ + H2O
-
 Pseudomonas sp. 4-aminobutanoate + NADH + H+
-
 ?
1.2.1.19 additional information very low activity with: succinate semialdehyde Pseudomonas sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.19 trimer 3 * 75000, SDS-PAGE Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.19 8
-
 3-aminopropanal Pseudomonas sp.
1.2.1.19 9 10 4-aminobutanal Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.19 NAD+
-
 Pseudomonas sp.