EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.298 | 3-hydroxypropanoate + NADP+ | Chloroflexus aurantiacus | - |
malonate-semialdehyde + NADPH + H+ | - |
r | |
1.1.1.298 | malonate-semialdehyde + NADPH + H+ | Chloroflexus aurantiacus | - |
3-hydroxypropionate + NADP+ | - |
r | |
1.1.1.298 | additional information | Chloroflexus aurantiacus | enzyme is part of an autotrophic CO2 fixation pathway in which acetyl-CoA is carboxylated and reductively converted via 3-hydroxypropionate to propionyl-CoA. Propionyl-CoA is carboxylated and converted via succinyl-CoA and CoA transfer to malyl-CoA. Malyl-CoA is cleaved to acetyl-CoA and glyoxylate. Thereby, the first CO, acceptor molecule acetyl-CoA is regenerated, completing the cycle and the net CO, fixation product glyoxylate is released | ? | - |
? | |
1.2.1.18 | malonyl-CoA + NADPH | Chloroflexus aurantiacus | enzyme is involved in the autotrophic CO2 fixation pathway, the 3-hydroxypropionate cycle | malonate-semialdehyde + CoA + NADP+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.298 | Chloroflexus aurantiacus | - |
- |
- |
1.2.1.18 | Chloroflexus aurantiacus | - |
- |
- |
1.2.1.75 | Chloroflexus aurantiacus | - |
- |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.298 | 0.002 | - |
substrate 3-hydroxypropionate, pH 9.0, 45°C | Chloroflexus aurantiacus |
1.1.1.298 | 0.04 | - |
substrate malonate-semialdehyde, pH 9.0, 45°C | Chloroflexus aurantiacus |
1.2.1.75 | 80 | - |
45°C, pH 7.8 | Chloroflexus aurantiacus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.298 | 3-hydroxypropanoate + NADP+ | - |
Chloroflexus aurantiacus | malonate-semialdehyde + NADPH + H+ | - |
r | |
1.1.1.298 | malonate-semialdehyde + NADPH + H+ | - |
Chloroflexus aurantiacus | 3-hydroxypropionate + NADP+ | - |
r | |
1.1.1.298 | additional information | enzyme is part of an autotrophic CO2 fixation pathway in which acetyl-CoA is carboxylated and reductively converted via 3-hydroxypropionate to propionyl-CoA. Propionyl-CoA is carboxylated and converted via succinyl-CoA and CoA transfer to malyl-CoA. Malyl-CoA is cleaved to acetyl-CoA and glyoxylate. Thereby, the first CO, acceptor molecule acetyl-CoA is regenerated, completing the cycle and the net CO, fixation product glyoxylate is released | Chloroflexus aurantiacus | ? | - |
? | |
1.2.1.18 | malonyl-CoA + NADPH | - |
Chloroflexus aurantiacus | malonate-semialdehyde + CoA + NADP+ | - |
? | |
1.2.1.18 | malonyl-CoA + NADPH | enzyme is involved in the autotrophic CO2 fixation pathway, the 3-hydroxypropionate cycle | Chloroflexus aurantiacus | malonate-semialdehyde + CoA + NADP+ | - |
? | |
1.2.1.75 | malonyl-CoA + NADPH + H+ | - |
Chloroflexus aurantiacus | malonate semialdehyde + NADP+ + CoA | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.1.75 | malonate-semialdehyde dehydrogenase | - |
Chloroflexus aurantiacus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.298 | additional information | no cofactor: NAD+ | Chloroflexus aurantiacus | |
1.1.1.298 | NADP+ | - |
Chloroflexus aurantiacus | |
1.1.1.298 | NADPH | - |
Chloroflexus aurantiacus | |
1.2.1.18 | NADPH | cofactor | Chloroflexus aurantiacus | |
1.2.1.18 | NADPH | no activity with NADH | Chloroflexus aurantiacus | |
1.2.1.75 | additional information | no cofactor: NADH | Chloroflexus aurantiacus | |
1.2.1.75 | NADPH | - |
Chloroflexus aurantiacus |