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Literature summary extracted from

  • Strauss, G.; Fuchs, G.
    Enzymes of a novel autotrophic carbon dioxide fixation pathway in the phototrophic bacterium Chloroflexus aurantiacus, the 3-hydroxypropionate cycle (1993), Eur. J. Biochem., 215, 633-643.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.298 3-hydroxypropanoate + NADP+ Chloroflexus aurantiacus
-
malonate-semialdehyde + NADPH + H+
-
r
1.1.1.298 malonate-semialdehyde + NADPH + H+ Chloroflexus aurantiacus
-
3-hydroxypropionate + NADP+
-
r
1.1.1.298 additional information Chloroflexus aurantiacus enzyme is part of an autotrophic CO2 fixation pathway in which acetyl-CoA is carboxylated and reductively converted via 3-hydroxypropionate to propionyl-CoA. Propionyl-CoA is carboxylated and converted via succinyl-CoA and CoA transfer to malyl-CoA. Malyl-CoA is cleaved to acetyl-CoA and glyoxylate. Thereby, the first CO, acceptor molecule acetyl-CoA is regenerated, completing the cycle and the net CO, fixation product glyoxylate is released ?
-
?
1.2.1.18 malonyl-CoA + NADPH Chloroflexus aurantiacus enzyme is involved in the autotrophic CO2 fixation pathway, the 3-hydroxypropionate cycle malonate-semialdehyde + CoA + NADP+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.298 Chloroflexus aurantiacus
-
-
-
1.2.1.18 Chloroflexus aurantiacus
-
-
-
1.2.1.75 Chloroflexus aurantiacus
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.298 0.002
-
substrate 3-hydroxypropionate, pH 9.0, 45°C Chloroflexus aurantiacus
1.1.1.298 0.04
-
substrate malonate-semialdehyde, pH 9.0, 45°C Chloroflexus aurantiacus
1.2.1.75 80
-
45°C, pH 7.8 Chloroflexus aurantiacus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.298 3-hydroxypropanoate + NADP+
-
Chloroflexus aurantiacus malonate-semialdehyde + NADPH + H+
-
r
1.1.1.298 malonate-semialdehyde + NADPH + H+
-
Chloroflexus aurantiacus 3-hydroxypropionate + NADP+
-
r
1.1.1.298 additional information enzyme is part of an autotrophic CO2 fixation pathway in which acetyl-CoA is carboxylated and reductively converted via 3-hydroxypropionate to propionyl-CoA. Propionyl-CoA is carboxylated and converted via succinyl-CoA and CoA transfer to malyl-CoA. Malyl-CoA is cleaved to acetyl-CoA and glyoxylate. Thereby, the first CO, acceptor molecule acetyl-CoA is regenerated, completing the cycle and the net CO, fixation product glyoxylate is released Chloroflexus aurantiacus ?
-
?
1.2.1.18 malonyl-CoA + NADPH
-
Chloroflexus aurantiacus malonate-semialdehyde + CoA + NADP+
-
?
1.2.1.18 malonyl-CoA + NADPH enzyme is involved in the autotrophic CO2 fixation pathway, the 3-hydroxypropionate cycle Chloroflexus aurantiacus malonate-semialdehyde + CoA + NADP+
-
?
1.2.1.75 malonyl-CoA + NADPH + H+
-
Chloroflexus aurantiacus malonate semialdehyde + NADP+ + CoA
-
?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.75 malonate-semialdehyde dehydrogenase
-
Chloroflexus aurantiacus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.298 additional information no cofactor: NAD+ Chloroflexus aurantiacus
1.1.1.298 NADP+
-
Chloroflexus aurantiacus
1.1.1.298 NADPH
-
Chloroflexus aurantiacus
1.2.1.18 NADPH cofactor Chloroflexus aurantiacus
1.2.1.18 NADPH no activity with NADH Chloroflexus aurantiacus
1.2.1.75 additional information no cofactor: NADH Chloroflexus aurantiacus
1.2.1.75 NADPH
-
Chloroflexus aurantiacus