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Literature summary extracted from

  • Lutke-Eversloh, T.; Steinbuchel, A.
    Biochemical and molecular characterization of a succinate semialdehyde dehydrogenase involved in the catabolism of 4-hydroxybutyric acid in Ralstonia eutropha (1999), FEMS Microbiol. Lett., 181, 63-71.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.16 gabD gene cloned, expression in Escherichia coli Cupriavidus necator
1.2.1.79 expression in Escherichia coli Cupriavidus necator

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.79 additional information
-
 additional information enzyme does not obey Michaelis-Menten kinetics Cupriavidus necator

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.79 5022
-
 x * 5022, calculated, x * 51000, SDS-PAGE Cupriavidus necator
1.2.1.79 51000
-
 x * 5022, calculated, x * 51000, SDS-PAGE Cupriavidus necator

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.16 succinate semialdehyde + NADP+ + H2O Cupriavidus necator involved in the degradation of gamma-aminobutyrate succinate + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.16 Cupriavidus necator Q9RBF6
-
-
1.2.1.79 Cupriavidus necator Q9RBF6
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.16 additional information
-
-
 Cupriavidus necator
1.2.1.16 0.0038
-
 H16-strain, coenzyme NAD+ Cupriavidus necator
1.2.1.16 0.0097
-
 SK4580-strain, coenzyme NAD+ Cupriavidus necator
1.2.1.16 0.0105
-
 HF39-strain, coenzyme NAD+ Cupriavidus necator
1.2.1.16 0.0172
-
 SHF3-strain, coenzyme NAD+ Cupriavidus necator
1.2.1.16 0.0235
-
 SK4580-strain, coenzyme NADP+ Cupriavidus necator
1.2.1.16 0.0253
-
 H16-strain, coenzyme NADP+ Cupriavidus necator
1.2.1.16 0.0319
-
 SK4575-strain, coenzyme NAD+ Cupriavidus necator
1.2.1.16 0.0355
-
 SK4575-strain, coenzyme NADP+ Cupriavidus necator
1.2.1.16 0.0409
-
 HF39-strain, coenzyme NADP+ Cupriavidus necator
1.2.1.16 0.0457
-
 SHF3-strain, coenzyme NADP+ Cupriavidus necator
1.2.1.79 14.9
-
 cosubstrate NAD+, pH 11.0, 30°C Cupriavidus necator
1.2.1.79 132.1
-
 cosubstrate NADP+, pH 11.0, 30°C Cupriavidus necator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.16 succinate semialdehyde + NADP+ + H2O
-
 Cupriavidus necator succinate + NADPH + H+
-
 ?
1.2.1.16 succinate semialdehyde + NADP+ + H2O involved in the degradation of gamma-aminobutyrate Cupriavidus necator succinate + NADPH + H+
-
 ?
1.2.1.79 succinate semialdehyde + NAD+ + H2O about 11% of the activity with NADP+ Cupriavidus necator succinate + NADH + 2 H+
-
 ?
1.2.1.79 succinate semialdehyde + NADP+ + H2O
-
 Cupriavidus necator succinate + NADPH + 2 H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.79 ? x * 5022, calculated, x * 51000, SDS-PAGE Cupriavidus necator

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.16 31
-
-
 Cupriavidus necator
1.2.1.79 31
-
-
 Cupriavidus necator

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.16 50
-
 10 min incubation, stable up to Cupriavidus necator
1.2.1.79 50
-
 10 min, stable Cupriavidus necator

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.16 11
-
 in 50 mM glycine-NaOH buffer Cupriavidus necator
1.2.1.79 11
-
-
 Cupriavidus necator

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.16 NAD+
-
 Cupriavidus necator
1.2.1.16 NADP+
-
 Cupriavidus necator
1.2.1.79 NAD+ about 11% of the activity with NADP+ Cupriavidus necator
1.2.1.79 NADP+
-
 Cupriavidus necator

Expression

EC Number Organism Comment Expression
1.2.1.79 Cupriavidus necator the expression of gabD is not specifically induced by either 4-hydroxybutanoic acid or gamma-aminobutanoate additional information

General Information

EC Number General Information Comment Organism
1.2.1.79 physiological function gene is disrupted in a transposon-induced mutant of Ralstonia eutropha exhibiting the phenotype 4-hydroxybutyric acid-leaky Cupriavidus necator