Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Tokunaga, M.; Nakano, Y.; Kitaoka, S.
    Separation and properties of the NAD-linked and NADP-linked isozymes of succinic semialdehyde dehydrogenase in Euglena gracilis z (1976), Biochim. Biophys. Acta, 429, 55-62.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.16 sulfhydryl reagents beta-mercaptoethanol and dithiotreitol activate Euglena gracilis
1.2.1.24 dithiothreitol 1 mM, 17% activation Euglena gracilis
1.2.1.79 dithiothreitol 1 mM, 78% activation Euglena gracilis

General Stability

EC Number General Stability Organism
1.2.1.16 ethyleneglycol, 30%, stabilizes Euglena gracilis
1.2.1.16 glycerol, 30%, stabilizes Euglena gracilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.16 5,5'-dithiobis(2-nitrobenzoate)
-
Euglena gracilis
1.2.1.16 glyoxylate
-
Euglena gracilis
1.2.1.16 N-ethylmaleimide
-
Euglena gracilis
1.2.1.16 p-chloromercuribenzoate
-
Euglena gracilis
1.2.1.16 potassium phosphate buffer
-
Euglena gracilis
1.2.1.16 Zn2+
-
Euglena gracilis
1.2.1.24 5,5'-dithiobis(2-nitrobenzoic acid) 0.01 mM, 66% residual activity Euglena gracilis
1.2.1.24 Ca2+
-
Euglena gracilis
1.2.1.24 Mg2+
-
Euglena gracilis
1.2.1.24 Mn2+
-
Euglena gracilis
1.2.1.24 N-ethylmaleimide 0.1 mM, complete loss of activity Euglena gracilis
1.2.1.24 Ni2+
-
Euglena gracilis
1.2.1.24 succinate semialdehyde substrate inhibition above 2 mM Euglena gracilis
1.2.1.24 Zn2+ 1 mM, 43% inhibition Euglena gracilis
1.2.1.79 5,5'-dithiobis(2-nitrobenzoic acid) 0.01 mM, 29% residual activity Euglena gracilis
1.2.1.79 Ca2+
-
Euglena gracilis
1.2.1.79 Mg2+
-
Euglena gracilis
1.2.1.79 Mn2+
-
Euglena gracilis
1.2.1.79 N-ethylmaleimide 0.1 mM, complete loss of activity Euglena gracilis
1.2.1.79 Ni2+
-
Euglena gracilis
1.2.1.79 succinate semialdehyde substrate inhibition above 1 mM Euglena gracilis
1.2.1.79 Zn2+ 1 mM, complete inhibition Euglena gracilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.16 0.038
-
NADP+
-
Euglena gracilis
1.2.1.16 1.06
-
succinate semialdehyde
-
Euglena gracilis
1.2.1.24 0.071
-
NAD+ pH 8.3, 35°C Euglena gracilis
1.2.1.24 0.16
-
succinate semialdehyde pH 8.3, 35°C Euglena gracilis
1.2.1.79 0.038
-
NADP+ pH 8.7, 35°C Euglena gracilis
1.2.1.79 1.06
-
succinate semialdehyde pH 8.7, 35°C Euglena gracilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.16 K+ slight activation Euglena gracilis
1.2.1.24 K+ 5 mM, 84% activation Euglena gracilis
1.2.1.79 K+ 5 mM, 10% activation Euglena gracilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.16 150000
-
gel filtration Euglena gracilis
1.2.1.24 150000
-
gel filtration Euglena gracilis

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.16 Euglena gracilis
-
-
-
1.2.1.24 Euglena gracilis
-
-
-
1.2.1.79 Euglena gracilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.16 ammonium sulfate fractionation and DEAE-cellulose Euglena gracilis

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.16 7.94
-
-
Euglena gracilis
1.2.1.24 3.25
-
pH 8.3, 35°C Euglena gracilis
1.2.1.79 7.9
-
pH 8.7, 35°C Euglena gracilis

Storage Stability

EC Number Storage Stability Organism
1.2.1.16 5°C, complete inactivation after 2 days Euglena gracilis
1.2.1.24 5°C, unstable unless 30% glycerol is added Euglena gracilis
1.2.1.79 5°C, unstable unless 30% glycerol is added Euglena gracilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.16 malonic semialdehyde + NADP+ + H2O hydrolyzed at 8.7% the rate of succinate semialdehyde Euglena gracilis malonate + NADPH + H+
-
?
1.2.1.16 succinate semialdehyde + NADP+ + H2O
-
Euglena gracilis succinate + NADPH + H+
-
?
1.2.1.24 malonate semialdehyde + NAD+ + H2O 3.8% of the activity with succinate semialdehyde Euglena gracilis malonate + NADH + 2 H+
-
?
1.2.1.24 additional information no substrate: glyoxylic acid, formic acid, formaldehyde, acetaldehyde, glyoxal, furfural and acrolein Euglena gracilis ?
-
?
1.2.1.24 succinate semialdehyde + NAD+ + H2O
-
Euglena gracilis succinate + NADH + 2 H+
-
?
1.2.1.79 malonate semialdehyde + NADP+ + H2O 8.7% of the activity with succinate semialdehyde Euglena gracilis malonate + NADPH + 2 H+
-
?
1.2.1.79 additional information no substrate: glyoxylic acid, formic acid, formaldehyde, acetaldehyde, glyoxal, furfural and acrolein Euglena gracilis ?
-
?
1.2.1.79 succinate semialdehyde + NADP+ + H2O
-
Euglena gracilis succinate + NADPH + 2 H+
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.16 35 45
-
Euglena gracilis
1.2.1.24 35 45
-
Euglena gracilis
1.2.1.79 35 45
-
Euglena gracilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.16 60
-
up to, in presence of 30% glycerol Euglena gracilis
1.2.1.24 60
-
stable up to Euglena gracilis
1.2.1.79 60
-
stable up to Euglena gracilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.16 8.7
-
-
Euglena gracilis
1.2.1.24 8.7
-
-
Euglena gracilis
1.2.1.79 8.7
-
-
Euglena gracilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.16 7.5 9.5
-
Euglena gracilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.16 NADP+ specific for Euglena gracilis
1.2.1.24 NAD+
-
Euglena gracilis
1.2.1.79 NADP+
-
Euglena gracilis

Expression

EC Number Organism Comment Expression
1.2.1.24 Euglena gracilis induction by growth on glutamate up
1.2.1.79 Euglena gracilis induction by growth on glutamate up