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Literature summary extracted from

  • Russo, A.D.; Rullo, R.; Masullo, M.; Ianniciello, G.; Arcari, P.; Bocchini, V.
    Glyceraldehyde 3-phosphate dehydrogenase in the hyperthermophilic archaeon Sulfolobus solfataricus: characterization and significance in glucose metabolism (1995), Biochem. Mol. Biol. Int., 36, 123-135.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.12 5
-
 NAD+ pH 8.5, 65°C Saccharolobus solfataricus
1.2.1.13 0.01
-
 NADPH
-
 Saccharolobus solfataricus
1.2.1.13 0.02
-
 NADP+
-
 Saccharolobus solfataricus
1.2.1.13 0.1
-
 NADP+
-
 Saccharolobus solfataricus
1.2.1.13 0.18
-
 NADH
-
 Saccharolobus solfataricus
1.2.1.13 5
-
 NAD+
-
 Saccharolobus solfataricus
1.2.1.13 88
-
 arsenate
-
 Saccharolobus solfataricus
1.2.1.13 182
-
 phosphate
-
 Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.12 41000
-
 4 * 41000, SDS-PAGE Saccharolobus solfataricus
1.2.1.12 155000
-
 gel filtration Saccharolobus solfataricus
1.2.1.13 41000
-
 4 * 41000, SDS-PAGE Saccharolobus solfataricus
1.2.1.13 155000
-
 gel filtration Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.12 D-glyceraldehyde 3-phosphate + phosphate + NAD+ Saccharolobus solfataricus
-
 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.12 Saccharolobus solfataricus P39460
-
-
1.2.1.13 Saccharolobus solfataricus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.12
-
 Saccharolobus solfataricus
1.2.1.13
-
 Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.12 9.2
-
 pH 8.5, 65°C Saccharolobus solfataricus
1.2.1.13 9.2
-
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.12 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Saccharolobus solfataricus 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?
1.2.1.12 D-glyceraldehyde 3-phosphate + phosphate + NAD+ the enzyme utilizes either NAD+ or NADP+ as coenzyme but its affinity for the latter is about 50fold higher Saccharolobus solfataricus 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 ?
1.2.1.13 D-glyceraldehyde 3-phosphate + arsenate + NADP+
-
 Saccharolobus solfataricus 3-phospho-D-glyceroyl arsenate + NADPH
-
 ?
1.2.1.13 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Saccharolobus solfataricus 3-phospho-D-glyceroyl phosphate + NADH
-
 r
1.2.1.13 D-glyceraldehyde 3-phosphate + phosphate + NADP+
-
 Saccharolobus solfataricus 3-phospho-D-glyceroyl phosphate + NADPH
-
 r

Subunits

EC Number Subunits Comment Organism
1.2.1.12 tetramer 4 * 41000, SDS-PAGE Saccharolobus solfataricus
1.2.1.13 tetramer 4 * 41000, SDS-PAGE Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.12 87
-
-
 Saccharolobus solfataricus
1.2.1.13 87
-
-
 Saccharolobus solfataricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.2.1.12 87
-
 activity decreases rapidly above 87°C Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.12 87
-
 half-life: 45 min Saccharolobus solfataricus
1.2.1.12 95
-
 complete inactivation after 30 min Saccharolobus solfataricus
1.2.1.13 70
-
 40 min, stable Saccharolobus solfataricus
1.2.1.13 87
-
 half-life: 45 min Saccharolobus solfataricus
1.2.1.13 95
-
 30 min, complete inactivation Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.12 20
-
 NAD+ pH 8.5, 65°C Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.12 8.5
-
-
 Saccharolobus solfataricus
1.2.1.13 8.5
-
 both directions Saccharolobus solfataricus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.12 NAD+ the enzyme utilizes either NAD+ or NADP+ as coenzyme but its affinity for NADP+ is about 50fold higher Saccharolobus solfataricus
1.2.1.13 NAD+ activity with NADP+ is about 50fold higher than that determined for NAD+ Saccharolobus solfataricus
1.2.1.13 NADP+ cofactor Saccharolobus solfataricus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.2.1.12 Saccharolobus solfataricus calculated from sequence
-
 8.7

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.2.1.12 0.25
-
 NAD+ pH 8.5, 65°C Saccharolobus solfataricus