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Literature summary extracted from

  • Roitel, O.; Sergienko, E.; Branlant, G.
    Dimers generated from tetrameric phosphorylating glyceraldehyde-3-phosphate dehydrogenase from Bacillus stearothermophilus are inactive but exhibit cooperativity in NAD binding (1999), Biochemistry, 38, 16084-16091.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.12 D186G behavior in NAD+ binding is similar to that of the wild type enzyme Geobacillus stearothermophilus
1.2.1.12 D186G/E276G positive cooperativity in binding the coenzyme NAD+ Geobacillus stearothermophilus
1.2.1.12 E276G behavior in NAD+ binding is similar to that of the wild type enzyme Geobacillus stearothermophilus
1.2.1.12 Y46G behavior in NAD+ binding is similar to that of the wild type enzyme Geobacillus stearothermophilus
1.2.1.12 Y46G/S48G positive cooperativity in binding the coenzyme NAD+ Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.12 0.04
-
 NAD+ mutant enzyme S48G Geobacillus stearothermophilus
1.2.1.12 0.05
-
 NAD+ wild-type enzyme Geobacillus stearothermophilus
1.2.1.12 0.07
-
 NAD+ mutant enzyme E276G Geobacillus stearothermophilus
1.2.1.12 0.33
-
 NAD+ mutant enzyme S186G Geobacillus stearothermophilus
1.2.1.12 0.8
-
 D-glyceraldehyde 3-phosphate mutant enzyme Y46G Geobacillus stearothermophilus
1.2.1.12 0.9
-
 D-glyceraldehyde 3-phosphate mutant enzyme E276G Geobacillus stearothermophilus
1.2.1.12 1
-
 D-glyceraldehyde 3-phosphate mutant enzyme D186G and mutant enzyme S48G Geobacillus stearothermophilus
1.2.1.12 1.1
-
 D-glyceraldehyde 3-phosphate wild-type enzyme Geobacillus stearothermophilus
1.2.1.12 7
-
 phosphate mutant enzyme E276G and mutant enzyme S48G Geobacillus stearothermophilus
1.2.1.12 8
-
 phosphate mutant enzyme Y46G Geobacillus stearothermophilus
1.2.1.12 18
-
 phosphate mutant enzyme D186G Geobacillus stearothermophilus
1.2.1.12 37
-
 phosphate wild-type enzyme Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.12 Geobacillus stearothermophilus P00362
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.12 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Geobacillus stearothermophilus 3-phospho-D-glyceroyl phosphate + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.12 More dimers generated from the tetrameric enzyme are inactive but exhibit cooperativity in NAD+ binding Geobacillus stearothermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.12 additional information
-
 additional information
-
 Geobacillus stearothermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.12 NAD+ dimers generated from the tetrameric enzyme are inactive but exhibit cooperativity in NAD+ binding Geobacillus stearothermophilus