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Literature summary extracted from

  • Greene, F.C.; Feeney, R.E.
    Properties of muscle glyceraldehyde-3-phosphate dehydrogenase from the cold-adapted antarctic fish Dissostichus mawsoni (1970), Biochim. Biophys. Acta, 220, 430-442.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.12 AMP 18 mM, only 5% loss of activity Dissostichus mawsoni
1.2.1.12 ATP 1 mM, 25% loss of activity Dissostichus mawsoni
1.2.1.12 D-glyceraldehyde 3-phosphate substrate inhibition Dissostichus mawsoni

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.12 0.018
-
 NAD+
-
 Oryctolagus cuniculus
1.2.1.12 0.0435
-
 thio-NAD+
-
 Oryctolagus cuniculus
1.2.1.12 0.076
-
 NAD+
-
 Dissostichus mawsoni
1.2.1.12 0.158
-
 3-acetyl-NAD+
-
 Oryctolagus cuniculus
1.2.1.12 0.23
-
 D-glyceraldehyde 3-phosphate
-
 Dissostichus mawsoni
1.2.1.12 0.235
-
 thio-NAD+
-
 Dissostichus mawsoni
1.2.1.12 0.62
-
 3-acetyl-NAD+
-
 Dissostichus mawsoni
1.2.1.12 1.43
-
 3-acetylpyridine hypoxanthine nucleotide
-
 Oryctolagus cuniculus
1.2.1.12 8.15
-
 3-acetylpyridine hypoxanthine nucleotide
-
 Dissostichus mawsoni

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.12 142000
-
 low speed equilibrium sedimentation Dissostichus mawsoni

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.12 Dissostichus mawsoni
-
-
-
1.2.1.12 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.12
-
 Dissostichus mawsoni

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.12 muscle
-
 Oryctolagus cuniculus
-
1.2.1.12 muscle skeletal muscle Dissostichus mawsoni
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.12 D-glyceraldehyde 3-phosphate + 3-acetylpyridine hypoxanthine nucleotide + phosphate 1.3% of the activity with NAD+ Dissostichus mawsoni 3-phospho-D-glyceroyl phosphate + ?
-
 ?
1.2.1.12 D-glyceraldehyde 3-phosphate + 3-acetylpyridine hypoxanthine nucleotide + phosphate 3.2% of activity with NAD+ Oryctolagus cuniculus 3-phospho-D-glyceroyl phosphate + ?
-
 ?
1.2.1.12 D-glyceraldehyde 3-phosphate + 3-acetylpyridine NAD+ + phosphate 2.8% of the activity with NAD+ Dissostichus mawsoni 3-phospho-D-glyceroyl phosphate + ?
-
 ?
1.2.1.12 D-glyceraldehyde 3-phosphate + 3-acetylpyridine NAD+ + phosphate 4.5% of activity with NAD+ Oryctolagus cuniculus 3-phospho-D-glyceroyl phosphate + ?
-
 ?
1.2.1.12 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Oryctolagus cuniculus 3-phospho-D-glyceroyl phosphate + NADH
-
 ?
1.2.1.12 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Dissostichus mawsoni 3-phospho-D-glyceroyl phosphate + NADH
-
 ?
1.2.1.12 D-glyceraldehyde 3-phosphate + thio-NAD+ + phosphate 7.2% of the activity with NAD+ Dissostichus mawsoni 3-phospho-D-glyceroyl phosphate + thio-NADH
-
 ?
1.2.1.12 D-glyceraldehyde 3-phosphate + thio-NAD+ + phosphate 9.3% of activity with NAD+ Oryctolagus cuniculus 3-phospho-D-glyceroyl phosphate + thio-NADH
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.12 35
-
 30 min, stable up to Dissostichus mawsoni
1.2.1.12 55
-
 30 min, 50% loss of activity Dissostichus mawsoni

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.12 3-acetylpyridine hypoxanthine nucleotide 3.2% of activity with NAD+ Oryctolagus cuniculus
1.2.1.12 3-acetylpyridine hypoxanthine nucleotide 1.3% of the activity with NAD+ Dissostichus mawsoni
1.2.1.12 3-acetylpyridine-NAD+ 4.5% of activity with NAD+ as cofactor Oryctolagus cuniculus
1.2.1.12 3-acetylpyridine-NAD+ 2.8% of the activity with NAD+ Dissostichus mawsoni
1.2.1.12 NAD+
-
 Oryctolagus cuniculus
1.2.1.12 NAD+ contains 4 mol of NAD+ per mol of enzyme Dissostichus mawsoni
1.2.1.12 thio-NAD+ 9.3% of activity with NAD+ Oryctolagus cuniculus
1.2.1.12 thio-NAD+ 7.2% of the activity with NAD+ Dissostichus mawsoni