EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.2.1.12 | after addition of NAD+ to the apoprotein | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.12 | ADP | inhibition of esterase activity with p-nitrophenyl acetate | Escherichia coli | |
1.2.1.12 | AMP | inhibition of esterase activity with p-nitrophenyl acetate | Escherichia coli | |
1.2.1.12 | ATP | inhibition of esterase activity with p-nitrophenyl acetate | Escherichia coli | |
1.2.1.12 | iodoacetate | - |
Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.1.12 | 35000 | - |
x * 35000, high speed equilibrium sedimentation after treatment with 5 M guanidine hydrochloride containing 0.01 M dithiothreitol | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.12 | Escherichia coli | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.12 | D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Escherichia coli | 3-phospho-D-glyceroyl phosphate + NADH | - |
? | |
1.2.1.12 | p-nitrophenyl acetate + H2O | - |
Escherichia coli | p-nitrophenol + acetate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.1.12 | ? | x * 35000, high speed equilibrium sedimentation after treatment with 5 M guanidine hydrochloride containing 0.01 M dithiothreitol | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.12 | 8 | - |
50 mM Tris-chloride buffer and triethanolamine-chloride buffer | Escherichia coli |
1.2.1.12 | 8.8 | - |
50 mM sodium diphosphate buffer | Escherichia coli |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.12 | 7.5 | 8.5 | about 85% of maximal activity at pH 7.5 and pH 8.5, 50 mM Tris-chloride buffer. About 75% of maximal activity at pH 7.5 and pH 8.5, 50 mM triethanolamine-chloride buffer | Escherichia coli |
1.2.1.12 | 8.3 | 9.3 | 85% of maximal activity at pH 8.3 and pH 9.3, 50 mM sodium diphosphate buffer | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.12 | NAD+ | contains less than 0.3 mol of NAD+ per mol of native protein | Escherichia coli |