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Literature summary extracted from

  • D'Alessio, G.; Josse, J.
    Glyceraldehyde phosphate dehydrogenase of Escherichia coli. Structural and catalytic properties (1971), J. Biol. Chem., 246, 4326-4333.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.2.1.12 after addition of NAD+ to the apoprotein Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.12 ADP inhibition of esterase activity with p-nitrophenyl acetate Escherichia coli
1.2.1.12 AMP inhibition of esterase activity with p-nitrophenyl acetate Escherichia coli
1.2.1.12 ATP inhibition of esterase activity with p-nitrophenyl acetate Escherichia coli
1.2.1.12 iodoacetate
-
Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.12 35000
-
x * 35000, high speed equilibrium sedimentation after treatment with 5 M guanidine hydrochloride containing 0.01 M dithiothreitol Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.12 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.12 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
Escherichia coli 3-phospho-D-glyceroyl phosphate + NADH
-
?
1.2.1.12 p-nitrophenyl acetate + H2O
-
Escherichia coli p-nitrophenol + acetate
-
?

Subunits

EC Number Subunits Comment Organism
1.2.1.12 ? x * 35000, high speed equilibrium sedimentation after treatment with 5 M guanidine hydrochloride containing 0.01 M dithiothreitol Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.12 8
-
50 mM Tris-chloride buffer and triethanolamine-chloride buffer Escherichia coli
1.2.1.12 8.8
-
50 mM sodium diphosphate buffer Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.12 7.5 8.5 about 85% of maximal activity at pH 7.5 and pH 8.5, 50 mM Tris-chloride buffer. About 75% of maximal activity at pH 7.5 and pH 8.5, 50 mM triethanolamine-chloride buffer Escherichia coli
1.2.1.12 8.3 9.3 85% of maximal activity at pH 8.3 and pH 9.3, 50 mM sodium diphosphate buffer Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.12 NAD+ contains less than 0.3 mol of NAD+ per mol of native protein Escherichia coli