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Literature summary extracted from

  • Speranza, M.L.; Gozzer, C.
    Purification and properties of NAD+-dependent glyceraldehyde-3-phosphate dehydrogenase from spinach leaves (1978), Biochim. Biophys. Acta, 522, 32-42.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.12 0.25
-
 NAD+
-
 Spinacia oleracea
1.2.1.12 0.4
-
 D-glyceraldehyde 3-phosphate
-
 Spinacia oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.12 additional information
-
 Cys content is higher than that found in analogous enzymes Spinacia oleracea
1.2.1.12 14000
-
 x * 14000 + x * 37000, SDS-PAGE Spinacia oleracea
1.2.1.12 37000
-
 x * 14000 + x * 37000, SDS-PAGE Spinacia oleracea
1.2.1.12 150000
-
-
 Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.12 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.12
-
 Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.12 leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.12 additional information
-
-
 Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.12 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Spinacia oleracea 3-phospho-D-glyceroyl phosphate + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.12 ? x * 14000 + x * 37000, SDS-PAGE Spinacia oleracea

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.12 NAD+ contains about 1.8 mol NAD+ per mol of enzyme Spinacia oleracea