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Literature summary extracted from

  • Oshima, T.; Fujita, S.C.; Imahori, K.
    Glyceraldehyde-3-phosphate dehydrogenase from Thermus thermophilus (1982), Methods Enzymol., 89, 335-340.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.12 acetone activates Thermus thermophilus
1.2.1.12 ethanol 30% v/v, 5fold activation Thermus thermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.2.1.12
-
 Thermus thermophilus

General Stability

EC Number General Stability Organism
1.2.1.12 1% SDS, fully active Thermus thermophilus
1.2.1.12 8 M urea, 40% inactivation Thermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.12 CdCl2
-
 Thermus thermophilus
1.2.1.12 Cu2+
-
 Thermus thermophilus
1.2.1.12 HgCl2
-
 Thermus thermophilus
1.2.1.12 iodoacetamide
-
 Thermus thermophilus
1.2.1.12 iodoacetate
-
 Thermus thermophilus
1.2.1.12 Na2S4O6
-
 Thermus thermophilus
1.2.1.12 Sodium tetrathionate
-
 Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.12 0.1
-
 NAD+
-
 Thermus thermophilus
1.2.1.12 0.3
-
 D-glyceraldehyde 3-phosphate
-
 Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.12 (NH4)2SO4 0.045 M, 10fold activation Thermus thermophilus
1.2.1.12 CsCl activates Thermus thermophilus
1.2.1.12 K+ KCl, activates Thermus thermophilus
1.2.1.12 LiCl activates Thermus thermophilus
1.2.1.12 Na2SO4 up to 5fold activation Thermus thermophilus
1.2.1.12 NaCl up to 5fold activation Thermus thermophilus
1.2.1.12 NH4Cl 0.1 M, 25fold activation Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.12 33500
-
 4 * 33500, SDS-PAGE Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.12 Thermus thermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.12
-
 Thermus thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.12 5.4
-
 crystalline enzyme Thermus thermophilus

Storage Stability

EC Number Storage Stability Organism
1.2.1.12 -20°C, stable for at least 2 years Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.12 D-glyceraldehyde 3-phosphate + arsenate + NAD+ the product arseno-3-phosphoglycerate is decomposed readily to 3-phosphoglycerate Thermus thermophilus 3-phospho-D-glyceroyl arsenate + NADH
-
 ir
1.2.1.12 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Thermus thermophilus 3-phospho-D-glyceroyl phosphate + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.12 tetramer 4 * 33500, SDS-PAGE Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.12 90
-
 slow inactivation Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.12 8.3
-
-
 Thermus thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.12 NAD+
-
 Thermus thermophilus