Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Eyschen, J.; Vitoux, B.; Marraud, M.; Cung, M.T.; Branlant, G.
    Engineered glycolytic glyceraldehyde-3-phosphate dehydrogenase binds the anti conformation of NAD+ nicotinamide but does not experience A-specific hydride transfer (1999), Arch. Biochem. Biophys., 364, 219-227.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.12 N313T/Y317G dissociation constant for NAD+ is 300times higher than that of the wild-type enzyme. Conformational equilibrium between the syn and the anti forms with a preference for the anti conformer Escherichia coli
1.2.1.12 Y317A dissociation constant for NAD+ is 5times higher than that of the wild-type enzyme. Wild-type syn orientation of bound nicotinamide remains unchanged Escherichia coli
1.2.1.12 Y317G dissociation constant for NAD+ is 13times higher than that of the wild-type enzyme. Wild-type syn orientation of bound nicotinamide remains unchanged Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.12 0.045
-
 NAD+ wild-type enzyme Escherichia coli
1.2.1.12 0.05
-
 NAD+ mutant Y317A Escherichia coli
1.2.1.12 0.058
-
 NAD+ mutant N313T/Y317G Escherichia coli
1.2.1.12 0.19
-
 NAD+ mutant N313T and Y317G Escherichia coli
1.2.1.12 0.29
-
 D-glyceraldehyde 3-phosphate mutant N313T/Y317G Escherichia coli
1.2.1.12 0.42
-
 D-glyceraldehyde 3-phosphate mutant N313T Escherichia coli
1.2.1.12 0.53
-
 phosphate wild-type enzyme Escherichia coli
1.2.1.12 0.88
-
 D-glyceraldehyde 3-phosphate mutant Y317A Escherichia coli
1.2.1.12 0.89
-
 D-glyceraldehyde 3-phosphate wild-type enzyme Escherichia coli
1.2.1.12 2.05
-
 D-glyceraldehyde 3-phosphate mutant Y317G Escherichia coli
1.2.1.12 4.8
-
 phosphate mutant enzyme Y317A Escherichia coli
1.2.1.12 6.9
-
 phosphate mutant enzyme N313T/Y317G Escherichia coli
1.2.1.12 20
-
 phosphate mutant enzyme N313T Escherichia coli
1.2.1.12 35.1
-
 phosphate mutant enzyme Y317G Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.12 Escherichia coli P0A9B2 wild-type and mutant enzymes N313T, Y317A, Y317G and N313T/Y317G
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.2.1.12 D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH + H+ B-specific oxidoreductase Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.12 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Escherichia coli 3-phospho-D-glyceroyl phosphate + NADH
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.12 additional information
-
 additional information
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.12 NAD+
-
 Escherichia coli