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Literature summary extracted from

  • Zammit, A.; Copeland, L.
    Purification and immunological properties of NAD-dependent glyceraldehyde-3-phosphate dehydrogenase from the plant cytosol of nitrogen fixing soybean nodules (1991), J. Plant Physiol., 138, 44-50.
No PubMed abstract available

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.12 37000
-
4 * 37000, SDS-PAGE Glycine max
1.2.1.12 147000
-
gel filtration Glycine max

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.12 Bradyrhizobium japonicum
-
CB1809
-
1.2.1.12 Bradyrhizobium japonicum CB1809
-
CB1809
-
1.2.1.12 Glycine max
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.12
-
Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.12 bacteroid
-
Bradyrhizobium japonicum
-
1.2.1.12 nodule nitrogen fixing Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.12 145
-
-
Glycine max

Storage Stability

EC Number Storage Stability Organism
1.2.1.12 -20°C, 50 mM potassium phosphate buffer, pH 7.4, stable for at least 2 weeks Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.12 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
Bradyrhizobium japonicum 3-phospho-D-glyceroyl phosphate + NADH
-
?
1.2.1.12 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
Glycine max 3-phospho-D-glyceroyl phosphate + NADH
-
?
1.2.1.12 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
Bradyrhizobium japonicum CB1809 3-phospho-D-glyceroyl phosphate + NADH
-
?

Subunits

EC Number Subunits Comment Organism
1.2.1.12 tetramer 4 * 37000, SDS-PAGE Glycine max

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.12 NAD+
-
Bradyrhizobium japonicum
1.2.1.12 NAD+
-
Glycine max