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Literature summary extracted from

  • Lambeir, A.M.; Loiseau, A.M.; Kuntz, D.A.; Vellieux, F.M.; Michels, P.A.M.; Opperdoes, F.R.
    The cytosolic and glycosomal glyceraldehyde-3-phosphate dehydrogenase from Trypanosoma brucei. Kinetic properties and comparison with homologous enzymes (1991), Eur. J. Biochem., 198, 429-435.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.12 Agaricic acid 0.14 mM, 50% inhibition in absence of NAD+. 2 mM, 35% loss of activity in presence of 0.017 mM NAD+ Oryctolagus cuniculus
1.2.1.12 Agaricic acid glyoxysomal enzyme: 0.2 mM, 60% loss of activity. Cytosolic enzyme: 2 mM, less than 20% loss of activity Trypanosoma brucei
1.2.1.12 gossypol
-
 Trypanosoma brucei
1.2.1.12 pentalenolactone reversible Geobacillus stearothermophilus
1.2.1.12 pentalenolactone irreversible Oryctolagus cuniculus
1.2.1.12 pentalenolactone most potent inhibitor; reversible Trypanosoma brucei
1.2.1.12 suramin cyosolic enzyme: competitive with NAD+. Effect on Km-value and maximal veocity of glyoxysomal enzyme Trypanosoma brucei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.12 0.007
-
 NADH cytosolic enzyme Trypanosoma brucei
1.2.1.12 0.01
-
 NADH
-
 Homo sapiens
1.2.1.12 0.012
-
 NADH
-
 Oryctolagus cuniculus
1.2.1.12 0.012
-
 NADH
-
 Geobacillus stearothermophilus
1.2.1.12 0.02
-
 NADH glyoxysomal enzyme Trypanosoma brucei
1.2.1.12 0.034
-
 3-phospho-D-glyceroyl phosphate
-
 Geobacillus stearothermophilus
1.2.1.12 0.039
-
 NAD+
-
 Geobacillus stearothermophilus
1.2.1.12 0.04
-
 NAD+ cytosolic enzyme Trypanosoma brucei
1.2.1.12 0.05
-
 NAD+
-
 Homo sapiens
1.2.1.12 0.05
-
 NAD+
-
 Geobacillus stearothermophilus
1.2.1.12 0.06
-
 NAD+
-
 Oryctolagus cuniculus
1.2.1.12 0.07
-
 D-glyceraldehyde 3-phosphate
-
 Homo sapiens
1.2.1.12 0.082
-
 D-glyceraldehyde 3-phosphate
-
 Oryctolagus cuniculus
1.2.1.12 0.09
-
 D-glyceraldehyde 3-phosphate
-
 Oryctolagus cuniculus
1.2.1.12 0.09
-
 D-glyceraldehyde 3-phosphate
-
 Geobacillus stearothermophilus
1.2.1.12 0.1
-
 3-phospho-D-glyceroyl phosphate glyoxysomal enzyme Trypanosoma brucei
1.2.1.12 0.13
-
 3-phospho-D-glyceroyl phosphate
-
 Oryctolagus cuniculus
1.2.1.12 0.14
-
 3-phospho-D-glyceroyl phosphate
-
 Homo sapiens
1.2.1.12 0.14
-
 3-phospho-D-glyceroyl phosphate cytosolic enzyme Trypanosoma brucei
1.2.1.12 0.15
-
 D-glyceraldehyde 3-phosphate glyoxysomal enzyme Trypanosoma brucei
1.2.1.12 0.17
-
 D-glyceraldehyde 3-phosphate cytosolic enzyme Trypanosoma brucei
1.2.1.12 0.45
-
 NAD+ glyoxysomal enzyme Trypanosoma brucei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.12 cytosol
-
 Trypanosoma brucei 5829
-
1.2.1.12 glyoxysome
-
 Trypanosoma brucei 9514
-

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.12 Geobacillus stearothermophilus
-
-
-
1.2.1.12 Homo sapiens
-
-
-
1.2.1.12 Oryctolagus cuniculus
-
-
-
1.2.1.12 Trypanosoma brucei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.12 cytosolic isoenzyme Trypanosoma brucei

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.12 erythrocyte
-
 Homo sapiens
-
1.2.1.12 muscle
-
 Homo sapiens
-
1.2.1.12 muscle
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.12 additional information
-
-
 Geobacillus stearothermophilus
1.2.1.12 additional information
-
-
 Trypanosoma brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.12 3-phospho-D-glyceroyl phosphate + NADH
-
 Homo sapiens D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 r
1.2.1.12 3-phospho-D-glyceroyl phosphate + NADH
-
 Oryctolagus cuniculus D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 r
1.2.1.12 3-phospho-D-glyceroyl phosphate + NADH
-
 Geobacillus stearothermophilus D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 r
1.2.1.12 3-phospho-D-glyceroyl phosphate + NADH
-
 Trypanosoma brucei D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 r
1.2.1.12 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Homo sapiens 3-phospho-D-glyceroyl phosphate + NADH
-
 r
1.2.1.12 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Oryctolagus cuniculus 3-phospho-D-glyceroyl phosphate + NADH
-
 r
1.2.1.12 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Geobacillus stearothermophilus 3-phospho-D-glyceroyl phosphate + NADH
-
 r
1.2.1.12 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Trypanosoma brucei 3-phospho-D-glyceroyl phosphate + NADH
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.12 8.5 9 cytosolic enzyme and enzyme from glyoxysomes Trypanosoma brucei

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.12 NAD+
-
 Homo sapiens
1.2.1.12 NAD+
-
 Oryctolagus cuniculus
1.2.1.12 NAD+
-
 Geobacillus stearothermophilus
1.2.1.12 NAD+
-
 Trypanosoma brucei
1.2.1.12 NADH cofactor Homo sapiens
1.2.1.12 NADH cofactor Oryctolagus cuniculus
1.2.1.12 NADH cofactor Geobacillus stearothermophilus
1.2.1.12 NADH cofactor Trypanosoma brucei