BRENDA - Enzyme Database

Glutathione-dependent formaldehyde dehydrogenase/class III alcohol dehydrogenase: Further characterization of the rat liver enzyme

Koivusalo, M.; Uotila, L.; Adv. Exp. Med. Biol. 328, 465-474 (1993)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.284
12-oxododecanoic acid
linear competitive
Rattus norvegicus
1.1.1.284
S-formylglutathione
product inhibition with S-hydroxymethylglutathione
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.284
0.006
-
glutathione
-
Rattus norvegicus
1.1.1.284
0.125
-
glutathione monomethyl ester
-
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.284
Rattus norvegicus
-
class II alcohol dehydrogenase
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.284
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.284
formaldehyde + 6-mercaptohexanoate + NAD+
30% of the activity with glutathione
287897
Rattus norvegicus
S-formyl-6-mercaptohexenoate + NADH
-
-
-
?
1.1.1.284
formaldehyde + 8-mercaptooctanoate + NAD+
35% of the activity with glutathione
287897
Rattus norvegicus
S-formyl-8-mercaptooctanoate + NADH
-
-
-
?
1.1.1.284
formaldehyde + captopril + NAD+
8% of the activity with glutathione
287897
Rattus norvegicus
S-formylcaptopril + NADH
-
-
-
?
1.1.1.284
formaldehyde + glutathione + NAD+
the true substrate is a hemimercaptal, S-hydroxymethylglutathione, spontaneously formed from formaldehyde and glutathione
287897
Rattus norvegicus
S-formylglutathione + NADH + H+
-
287897
Rattus norvegicus
r
1.1.1.284
formaldehyde + glutathione + NADP+
-
287897
Rattus norvegicus
S-formylglutathione + NADPH + H+
-
287897
Rattus norvegicus
r
1.1.1.284
formaldehyde + glutathione monomethyl ester + NAD+
70% of the activity with glutathione
287897
Rattus norvegicus
S-formylglutathione monomethyl ester + NADH
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.284
NAD+
-
Rattus norvegicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.284
NAD+
-
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.284
12-oxododecanoic acid
linear competitive
Rattus norvegicus
1.1.1.284
S-formylglutathione
product inhibition with S-hydroxymethylglutathione
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.284
0.006
-
glutathione
-
Rattus norvegicus
1.1.1.284
0.125
-
glutathione monomethyl ester
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.284
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.284
formaldehyde + 6-mercaptohexanoate + NAD+
30% of the activity with glutathione
287897
Rattus norvegicus
S-formyl-6-mercaptohexenoate + NADH
-
-
-
?
1.1.1.284
formaldehyde + 8-mercaptooctanoate + NAD+
35% of the activity with glutathione
287897
Rattus norvegicus
S-formyl-8-mercaptooctanoate + NADH
-
-
-
?
1.1.1.284
formaldehyde + captopril + NAD+
8% of the activity with glutathione
287897
Rattus norvegicus
S-formylcaptopril + NADH
-
-
-
?
1.1.1.284
formaldehyde + glutathione + NAD+
the true substrate is a hemimercaptal, S-hydroxymethylglutathione, spontaneously formed from formaldehyde and glutathione
287897
Rattus norvegicus
S-formylglutathione + NADH + H+
-
287897
Rattus norvegicus
r
1.1.1.284
formaldehyde + glutathione + NADP+
-
287897
Rattus norvegicus
S-formylglutathione + NADPH + H+
-
287897
Rattus norvegicus
r
1.1.1.284
formaldehyde + glutathione monomethyl ester + NAD+
70% of the activity with glutathione
287897
Rattus norvegicus
S-formylglutathione monomethyl ester + NADH
-
-
-
?