BRENDA - Enzyme Database

Kinetic mechanism of human glutathione-dependent formaldehyde dehydrogenase

Sanghani, P.C.; Stone, C.L.; Ray, B.D.; Pindel, E.V.; Hurley, T.D.; Bosron, W.F.; Biochemistry 39, 10720-10729 (2000)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.284
12-oxododecanoic acid
substrate inhibition
Homo sapiens
1.1.1.284
NAD+
product inhibition
Homo sapiens
1.1.1.284
NADH
product inhibition
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.284
Zinc
zinc-dependent enzyme
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.284
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.284
-
Homo sapiens
Reaction
EC Number
Reaction
Commentary
Organism
1.1.1.284
S-(hydroxymethyl)glutathione + NAD(P)+ = S-formylglutathione + NAD(P)H + H+
random bi-bi mechanism for oxidation of S-hydroxymethylglutathione and 12-hydroxydodecanoic acid
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.284
12-hydroxydodedecanoic acid + glutathione + NAD+
-
287893
Homo sapiens
12-oxododecanoic acid + ?
-
287893
Homo sapiens
?
1.1.1.284
formaldehyde + glutathione + NAD+
the true substrate is a hemimercaptal, S-hydroxymethylglutathione, spontaneously formed from formaldehyde and glutathione
287893
Homo sapiens
S-formylglutathione + NADH + H+
-
287893
Homo sapiens
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.284
0.217
-
12-Hydroxydodecanoic acid
-
Homo sapiens
1.1.1.284
0.217
-
NAD+
reaction with 12-hydroxydodecanoic acid
Homo sapiens
1.1.1.284
1.04
-
NAD+
-
Homo sapiens
1.1.1.284
1.04
-
S-hydroxymethyl glutathione
-
Homo sapiens
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.284
NAD+
-
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.284
NAD+
-
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.284
12-oxododecanoic acid
substrate inhibition
Homo sapiens
1.1.1.284
NAD+
product inhibition
Homo sapiens
1.1.1.284
NADH
product inhibition
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.284
Zinc
zinc-dependent enzyme
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.284
-
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.284
12-hydroxydodedecanoic acid + glutathione + NAD+
-
287893
Homo sapiens
12-oxododecanoic acid + ?
-
287893
Homo sapiens
?
1.1.1.284
formaldehyde + glutathione + NAD+
the true substrate is a hemimercaptal, S-hydroxymethylglutathione, spontaneously formed from formaldehyde and glutathione
287893
Homo sapiens
S-formylglutathione + NADH + H+
-
287893
Homo sapiens
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.284
0.217
-
12-Hydroxydodecanoic acid
-
Homo sapiens
1.1.1.284
0.217
-
NAD+
reaction with 12-hydroxydodecanoic acid
Homo sapiens
1.1.1.284
1.04
-
NAD+
-
Homo sapiens
1.1.1.284
1.04
-
S-hydroxymethyl glutathione
-
Homo sapiens