BRENDA - Enzyme Database

Substrate specificity of bovine liver formaldehyde dehydrogenase

Pourmotabbed, T.; Creighton, D.J.; J. Biol. Chem. 261, 14240-14244 (1986)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.284
0.008
-
S-hydroxymethylglutathione
-
Bos taurus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.284
41000
-
2 * 41000, SDS-PAGE
Bos taurus
1.1.1.284
75000
78000
gel filtration
Bos taurus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.284
Bos taurus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.284
-
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.284
liver
-
Bos taurus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.284
additional information
-
-
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.284
formaldehyde + glutathione + NAD+
the true substrate is a hemimercaptal, S-hydroxymethylglutathione, spontaneously formed from formaldehyde and glutathione
287890
Bos taurus
S-formylglutathione + NADH + H+
-
287890
Bos taurus
?
1.1.1.284
formaldehyde + S-hydroxymethyl glutathione + NAD+
-
287890
Bos taurus
?
-
-
-
?
1.1.1.284
methylglyoxal + glutathione + NAD+
-
287890
Bos taurus
S-pyruvylglutathione + NADH
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.284
dimer
2 * 41000, SDS-PAGE
Bos taurus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.284
1.42
-
S-hydroxymethyl glutathione
-
Bos taurus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.284
NAD+
-
Bos taurus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.284
NAD+
-
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.284
0.008
-
S-hydroxymethylglutathione
-
Bos taurus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.284
41000
-
2 * 41000, SDS-PAGE
Bos taurus
1.1.1.284
75000
78000
gel filtration
Bos taurus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.284
-
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.284
liver
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.284
additional information
-
-
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.284
formaldehyde + glutathione + NAD+
the true substrate is a hemimercaptal, S-hydroxymethylglutathione, spontaneously formed from formaldehyde and glutathione
287890
Bos taurus
S-formylglutathione + NADH + H+
-
287890
Bos taurus
?
1.1.1.284
formaldehyde + S-hydroxymethyl glutathione + NAD+
-
287890
Bos taurus
?
-
-
-
?
1.1.1.284
methylglyoxal + glutathione + NAD+
-
287890
Bos taurus
S-pyruvylglutathione + NADH
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.284
dimer
2 * 41000, SDS-PAGE
Bos taurus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.284
1.42
-
S-hydroxymethyl glutathione
-
Bos taurus