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Literature summary extracted from

  • Pourmotabbed, T.; Creighton, D.J.
    Substrate specificity of bovine liver formaldehyde dehydrogenase (1986), J. Biol. Chem., 261, 14240-14244.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.284 0.008
-
 S-hydroxymethylglutathione
-
 Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.284 41000
-
 2 * 41000, SDS-PAGE Bos taurus
1.1.1.284 75000 78000 gel filtration Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.284 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.284
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.284 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.284 additional information
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.284 formaldehyde + glutathione + NAD+ the true substrate is a hemimercaptal, S-hydroxymethylglutathione, spontaneously formed from formaldehyde and glutathione Bos taurus S-formylglutathione + NADH + H+
-
 ?
1.1.1.284 formaldehyde + S-hydroxymethyl glutathione + NAD+
-
 Bos taurus ?
-
 ?
1.1.1.284 methylglyoxal + glutathione + NAD+
-
 Bos taurus S-pyruvylglutathione + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.284 dimer 2 * 41000, SDS-PAGE Bos taurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.284 1.42
-
 S-hydroxymethyl glutathione
-
 Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.284 NAD+
-
 Bos taurus