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Literature summary extracted from

  • Schuette, H.; Flossdorf, J.; Sahm, H.; Kula, M.R.
    Purification and properties of formaldehyde dehydrogenase and formate dehydrogenase from Candida boidinii (1976), Eur. J. Biochem., 62, 151-160.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.284 inactivated by freezing and thawing [Candida] boidinii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.284 Cd2+ 1 mM, complete inhibition [Candida] boidinii
1.1.1.284 CN- 1 mM, 42% inhibition [Candida] boidinii
1.1.1.284 Cu2+ 1 mM, complete inhibition [Candida] boidinii
1.1.1.284 Hg2+ 1 mM, complete inhibition [Candida] boidinii
1.1.1.284 iodoacetate 1 mM, 15% inhibition [Candida] boidinii
1.1.1.284 PCMB 1 mM, complete inhibition [Candida] boidinii
1.1.1.284 Zn2+ 1 mM, 50% inhibition [Candida] boidinii
1.17.1.9 CN-
-
 [Candida] boidinii
1.17.1.9 Cu2+
-
 [Candida] boidinii
1.17.1.9 Hg2+
-
 [Candida] boidinii
1.17.1.9 N3-
-
 [Candida] boidinii
1.17.1.9 NO2-
-
 [Candida] boidinii
1.17.1.9 NO3-
-
 [Candida] boidinii
1.17.1.9 OCN-
-
 [Candida] boidinii
1.17.1.9 PCMB
-
 [Candida] boidinii
1.17.1.9 SCN-
-
 [Candida] boidinii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.284 0.09
-
 NAD+
-
 [Candida] boidinii
1.1.1.284 0.13
-
 glutathione
-
 [Candida] boidinii
1.1.1.284 0.25
-
 formaldehyde
-
 [Candida] boidinii
1.1.1.284 1.2
-
 methylglyoxal
-
 [Candida] boidinii
1.17.1.9 0.09
-
 NAD+
-
 [Candida] boidinii
1.17.1.9 13
-
 formate
-
 [Candida] boidinii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.284 40000
-
 2 * 40000, SDS-PAGE [Candida] boidinii
1.1.1.284 80000
-
 equilibrium sedimentation [Candida] boidinii
1.17.1.9 36000
-
 2 * 36000, SDS-PAGE [Candida] boidinii
1.17.1.9 74000
-
 equilibrium sedimentation [Candida] boidinii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.284 formaldehyde + glutathione + NAD+ [Candida] boidinii enzyme is found only in methanol-grown cells and is absent in cells grown on ethanol or glucose as carbon source S-formylglutathione + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.284 [Candida] boidinii
-
-
-
1.17.1.9 [Candida] boidinii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.284
-
 [Candida] boidinii
1.17.1.9
-
 [Candida] boidinii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.284 47.9
-
-
 [Candida] boidinii
1.17.1.9 2.4
-
-
 [Candida] boidinii

Storage Stability

EC Number Storage Stability Organism
1.1.1.284 50% glycerol, stable for a long time [Candida] boidinii
1.17.1.9 4°C, stable for several weeks [Candida] boidinii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.284 formaldehyde + glutathione + NAD+
-
 [Candida] boidinii S-formylglutathione + NADH + H+
-
 ?
1.1.1.284 formaldehyde + glutathione + NAD+ enzyme is found only in methanol-grown cells and is absent in cells grown on ethanol or glucose as carbon source [Candida] boidinii S-formylglutathione + NADH + H+
-
 ?
1.1.1.284 methylglyoxal + glutathione + NAD+
-
 [Candida] boidinii S-pyruvylglutathione + NADH
-
 ?
1.17.1.9 formate + NAD+
-
 [Candida] boidinii CO2 + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.284 dimer 2 * 40000, SDS-PAGE [Candida] boidinii
1.17.1.9 dimer 2 * 36000, SDS-PAGE [Candida] boidinii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.17.1.9 55
-
-
 [Candida] boidinii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.284 7.5 9
-
 [Candida] boidinii
1.17.1.9 7.5 8.5
-
 [Candida] boidinii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.284 NAD+
-
 [Candida] boidinii
1.17.1.9 NAD+
-
 [Candida] boidinii