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Literature summary extracted from
- Formaldehyde dehydrogenase from human liver. Purification, properties, and evidence for the formation of glutathione thiol esters by the enzyme (1974), J. Biol. Chem., 249, 7653-7663.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.1.1.284 | one freezing at -20°C results in full inactivation | Homo sapiens |
| 1.1.1.284 | overnight dialysis to remove mercaptoethanol destroys 60% of its original activity | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.284 | AgNO3 | NADH protects more effectively than NAD+ | Homo sapiens |  |
| 1.1.1.284 | formaldehyde | - |
Homo sapiens | |
| 1.1.1.284 | Hg2+ | NADH protects more effectively than NAD+ | Homo sapiens | |
| 1.1.1.284 | iodoacetate | NADH protects more effectively than NAD+ | Homo sapiens | |
| 1.1.1.284 | methylglyoxal | - |
Homo sapiens | |
| 1.1.1.284 | NAD+ | inhibits reverse reaction with NAD+ or NADP+ | Homo sapiens | |
| 1.1.1.284 | NADH | inhibits reaction with formaldehyde, glutathione and NAD+ | Homo sapiens | |
| 1.1.1.284 | NADPH | inhibits reaction with formaldehyde, glutathione and NAD+ | Homo sapiens | |
| 1.1.1.284 | S-formylglutathione | inhibits reaction with formaldehyde, glutathione and NAD+ | Homo sapiens | |
| 1.1.1.284 | S-formylglutathione | product inhibition with S-hydroxymethylglutathione | Rattus norvegicus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.284 | 0.0042 | - |
NADH | pH 5.7 | Homo sapiens | |
| 1.1.1.284 | 0.0081 | - |
glutathione | pH 8.0 | Homo sapiens | |
| 1.1.1.284 | 0.009 | - |
NAD+ | pH 8.0 | Homo sapiens | |
| 1.1.1.284 | 0.0136 | - |
NAD+ | pH 5.7 | Homo sapiens | |
| 1.1.1.284 | 0.016 | - |
NADH | pH 8.0 | Homo sapiens | |
| 1.1.1.284 | 0.045 | - |
NADPH | pH 5.7 | Homo sapiens | |
| 1.1.1.284 | 0.28 | - |
S-formylglutathione | pH 5.7, with NADPH as cofactor | Homo sapiens | |
| 1.1.1.284 | 0.3 | - |
S-formylglutathione | pH 5.7, with NADH as cofactor | Homo sapiens | |
| 1.1.1.284 | 0.57 | - |
NADP+ | pH 5.7 | Homo sapiens | |
| 1.1.1.284 | 4 | - |
pyruvylglutathione | pH 5.7, with NADH as cofactor | Homo sapiens | |
| 1.1.1.284 | 9.5 | - |
NADP+ | pH 8.0 | Homo sapiens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.1.1.284 | cytosol | - |
Homo sapiens | 5829 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.284 | 81400 | - |
gel filtration | Homo sapiens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.284 | Bos taurus | - |
- |
- |
| 1.1.1.284 | Homo sapiens | - |
- |
- |
| 1.1.1.284 | Mus musculus | - |
- |
- |
| 1.1.1.284 | Oryctolagus cuniculus | - |
- |
- |
| 1.1.1.284 | Ovis aries | - |
- |
- |
| 1.1.1.284 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.284 | - |
Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.1.1.284 | adrenal gland | - |
Bos taurus | - |
| 1.1.1.284 | ascites tumor cell | - |
Mus musculus | - |
| 1.1.1.284 | brain | - |
Homo sapiens | - |
| 1.1.1.284 | brain | - |
Rattus norvegicus | - |
| 1.1.1.284 | brain | - |
Bos taurus | - |
| 1.1.1.284 | brain | - |
Oryctolagus cuniculus | - |
| 1.1.1.284 | cardiac muscle | - |
Rattus norvegicus | - |
| 1.1.1.284 | kidney | - |
Rattus norvegicus | - |
| 1.1.1.284 | liver | - |
Mus musculus | - |
| 1.1.1.284 | liver | - |
Homo sapiens | - |
| 1.1.1.284 | liver | - |
Rattus norvegicus | - |
| 1.1.1.284 | liver | - |
Bos taurus | - |
| 1.1.1.284 | liver | - |
Oryctolagus cuniculus | - |
| 1.1.1.284 | liver | - |
Ovis aries | - |
| 1.1.1.284 | skeletal muscle | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.284 | 3.2 | - |
- |
Homo sapiens |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.1.284 | -70°C, in presence of 2 mM mercaptoethanol, 50% loss of activity | Homo sapiens |
| 1.1.1.284 | 0°C, 50% loss of activity after 1 month | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.284 | 3-ethoxy-2-hydroxybutyraldehyde + glutathione + NAD+ | - |
Homo sapiens | S-(3-ethoxy-2-hydroxybutyryl)-glutathione + NADH + H+ | - |
? | |
| 1.1.1.284 | formaldehyde + glutathione + NAD+ | - |
Mus musculus | S-formylglutathione + NADH + H+ | - |
? | |
| 1.1.1.284 | formaldehyde + glutathione + NAD+ | - |
Homo sapiens | S-formylglutathione + NADH + H+ | - |
r | |
| 1.1.1.284 | formaldehyde + glutathione + NAD+ | - |
Rattus norvegicus | S-formylglutathione + NADH + H+ | - |
r | |
| 1.1.1.284 | formaldehyde + glutathione + NAD+ | - |
Bos taurus | S-formylglutathione + NADH + H+ | - |
? | |
| 1.1.1.284 | formaldehyde + glutathione + NAD+ | - |
Oryctolagus cuniculus | S-formylglutathione + NADH + H+ | - |
? | |
| 1.1.1.284 | formaldehyde + glutathione + NAD+ | - |
Ovis aries | S-formylglutathione + NADH + H+ | - |
? | |
| 1.1.1.284 | formaldehyde + glutathione + NADP+ | - |
Homo sapiens | S-formylglutathione + NADPH + H+ | - |
r | |
| 1.1.1.284 | formaldehyde + glutathione + NADP+ | - |
Rattus norvegicus | S-formylglutathione + NADPH + H+ | - |
r | |
| 1.1.1.284 | glyoxal + glutathione + NAD+ | - |
Homo sapiens | S-oxoacetylglutathione + NADH | - |
? | |
| 1.1.1.284 | hydroxypyruvaldehyde + glutathione + NAD+ | - |
Homo sapiens | S-hydroxypyruvylglutathione + NADH | - |
? | |
| 1.1.1.284 | methylglyoxal + glutathione + NAD+ | - |
Homo sapiens | S-pyruvylglutathione + NADH | - |
? | |
| 1.1.1.284 | methylglyoxal + glutathione + NAD+ | - |
Bos taurus | S-pyruvylglutathione + NADH | - |
? | |
| 1.1.1.284 | pyruvylglutathione + NADH + H+ | - |
Homo sapiens | methylglyoxal + NAD+ | - |
r | |
| 1.1.1.284 | S-formylglutathione + NADH | - |
Homo sapiens | formaldehyde + glutathione + NAD+ | - |
r | |
| 1.1.1.284 | S-formylglutathione + NADH | - |
Rattus norvegicus | formaldehyde + glutathione + NAD+ | - |
r | |
| 1.1.1.284 | S-formylglutathione + NADPH | - |
Homo sapiens | formaldehyde + glutathione + NADP+ | - |
r | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.284 | 37 | - |
15 min, without NADH, 40% loss of activity | Homo sapiens |
| 1.1.1.284 | 64 | - |
15 min, in presence of NADH, 40% loss of activity | Homo sapiens |
| 1.1.1.284 | 68 | - |
15 min, in presence of NADH, 80% loss of activity | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.284 | 5.7 | - |
reaction with S-formylglutathione and NADH | Homo sapiens |
| 1.1.1.284 | 6 | - |
reaction with NADP+ as cofactor | Homo sapiens |
| 1.1.1.284 | 8 | - |
reaction with NAD+ as cofactor | Homo sapiens |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.284 | NAD+ | - |
Mus musculus | |
| 1.1.1.284 | NAD+ | - |
Homo sapiens | |
| 1.1.1.284 | NAD+ | - |
Rattus norvegicus | |
| 1.1.1.284 | NAD+ | - |
Bos taurus | |
| 1.1.1.284 | NAD+ | - |
Oryctolagus cuniculus | |
| 1.1.1.284 | NAD+ | - |
Ovis aries | |
| 1.1.1.284 | NADH | - |
Homo sapiens | |
| 1.1.1.284 | NADPH | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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