BRENDA - Enzyme Database

Formaldehyde dehydrogenase from human liver. Purification, properties, and evidence for the formation of glutathione thiol esters by the enzyme

Uotila, L.; Koivusalo, M.; J. Biol. Chem. 249, 7653-7663 (1974)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
1.1.1.284
one freezing at -20°C results in full inactivation
Homo sapiens
1.1.1.284
overnight dialysis to remove mercaptoethanol destroys 60% of its original activity
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.284
AgNO3
NADH protects more effectively than NAD+
Homo sapiens
1.1.1.284
formaldehyde
-
Homo sapiens
1.1.1.284
Hg2+
NADH protects more effectively than NAD+
Homo sapiens
1.1.1.284
iodoacetate
NADH protects more effectively than NAD+
Homo sapiens
1.1.1.284
methylglyoxal
-
Homo sapiens
1.1.1.284
NAD+
inhibits reverse reaction with NAD+ or NADP+
Homo sapiens
1.1.1.284
NADH
inhibits reaction with formaldehyde, glutathione and NAD+
Homo sapiens
1.1.1.284
NADPH
inhibits reaction with formaldehyde, glutathione and NAD+
Homo sapiens
1.1.1.284
S-formylglutathione
inhibits reaction with formaldehyde, glutathione and NAD+
Homo sapiens
1.1.1.284
S-formylglutathione
product inhibition with S-hydroxymethylglutathione
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.284
0.0042
-
NADH
pH 5.7
Homo sapiens
1.1.1.284
0.0081
-
glutathione
pH 8.0
Homo sapiens
1.1.1.284
0.009
-
NAD+
pH 8.0
Homo sapiens
1.1.1.284
0.0136
-
NAD+
pH 5.7
Homo sapiens
1.1.1.284
0.016
-
NADH
pH 8.0
Homo sapiens
1.1.1.284
0.045
-
NADPH
pH 5.7
Homo sapiens
1.1.1.284
0.28
-
S-formylglutathione
pH 5.7, with NADPH as cofactor
Homo sapiens
1.1.1.284
0.3
-
S-formylglutathione
pH 5.7, with NADH as cofactor
Homo sapiens
1.1.1.284
0.57
-
NADP+
pH 5.7
Homo sapiens
1.1.1.284
4
-
pyruvylglutathione
pH 5.7, with NADH as cofactor
Homo sapiens
1.1.1.284
9.5
-
NADP+
pH 8.0
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.284
cytosol
-
Homo sapiens
5829
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.284
81400
-
gel filtration
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.284
Bos taurus
-
-
-
1.1.1.284
Homo sapiens
-
-
-
1.1.1.284
Mus musculus
-
-
-
1.1.1.284
Oryctolagus cuniculus
-
-
-
1.1.1.284
Ovis aries
-
-
-
1.1.1.284
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.284
-
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.284
adrenal gland
-
Bos taurus
-
1.1.1.284
ascites tumor cell
-
Mus musculus
-
1.1.1.284
brain
-
Bos taurus
-
1.1.1.284
brain
-
Oryctolagus cuniculus
-
1.1.1.284
brain
-
Rattus norvegicus
-
1.1.1.284
brain
-
Homo sapiens
-
1.1.1.284
cardiac muscle
-
Rattus norvegicus
-
1.1.1.284
kidney
-
Rattus norvegicus
-
1.1.1.284
liver
-
Mus musculus
-
1.1.1.284
liver
-
Ovis aries
-
1.1.1.284
liver
-
Bos taurus
-
1.1.1.284
liver
-
Oryctolagus cuniculus
-
1.1.1.284
liver
-
Rattus norvegicus
-
1.1.1.284
liver
-
Homo sapiens
-
1.1.1.284
skeletal muscle
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.284
3.2
-
-
Homo sapiens
Storage Stability
EC Number
Storage Stability
Organism
1.1.1.284
-70°C, in presence of 2 mM mercaptoethanol, 50% loss of activity
Homo sapiens
1.1.1.284
0°C, 50% loss of activity after 1 month
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.284
3-ethoxy-2-hydroxybutyraldehyde + glutathione + NAD+
-
287888
Homo sapiens
S-(3-ethoxy-2-hydroxybutyryl)-glutathione + NADH + H+
-
-
-
?
1.1.1.284
formaldehyde + glutathione + NAD+
-
287888
Mus musculus
S-formylglutathione + NADH + H+
-
287888
Mus musculus
-
1.1.1.284
formaldehyde + glutathione + NAD+
-
287888
Homo sapiens
S-formylglutathione + NADH + H+
-
287888
Homo sapiens
r
1.1.1.284
formaldehyde + glutathione + NAD+
-
287888
Rattus norvegicus
S-formylglutathione + NADH + H+
-
287888
Rattus norvegicus
r
1.1.1.284
formaldehyde + glutathione + NAD+
-
287888
Bos taurus
S-formylglutathione + NADH + H+
-
287888
Bos taurus
-
1.1.1.284
formaldehyde + glutathione + NAD+
-
287888
Oryctolagus cuniculus
S-formylglutathione + NADH + H+
-
287888
Oryctolagus cuniculus
-
1.1.1.284
formaldehyde + glutathione + NAD+
-
287888
Ovis aries
S-formylglutathione + NADH + H+
-
287888
Ovis aries
-
1.1.1.284
formaldehyde + glutathione + NADP+
-
287888
Homo sapiens
S-formylglutathione + NADPH + H+
-
287888
Homo sapiens
r
1.1.1.284
formaldehyde + glutathione + NADP+
-
287888
Rattus norvegicus
S-formylglutathione + NADPH + H+
-
287888
Rattus norvegicus
r
1.1.1.284
glyoxal + glutathione + NAD+
-
287888
Homo sapiens
S-oxoacetylglutathione + NADH
-
-
-
?
1.1.1.284
hydroxypyruvaldehyde + glutathione + NAD+
-
287888
Homo sapiens
S-hydroxypyruvylglutathione + NADH
-
-
-
?
1.1.1.284
methylglyoxal + glutathione + NAD+
-
287888
Homo sapiens
S-pyruvylglutathione + NADH
-
-
-
?
1.1.1.284
methylglyoxal + glutathione + NAD+
-
287888
Bos taurus
S-pyruvylglutathione + NADH
-
-
-
?
1.1.1.284
S-formylglutathione + NADH
-
287888
Homo sapiens
formaldehyde + glutathione + NAD+
-
287888
Homo sapiens
r
1.1.1.284
S-formylglutathione + NADH
-
287888
Rattus norvegicus
formaldehyde + glutathione + NAD+
-
287888
Rattus norvegicus
r
1.1.1.284
S-formylglutathione + NADPH
-
287888
Homo sapiens
formaldehyde + glutathione + NADP+
-
287888
Homo sapiens
r
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.284
37
-
15 min, without NADH, 40% loss of activity
Homo sapiens
1.1.1.284
64
-
15 min, in presence of NADH, 40% loss of activity
Homo sapiens
1.1.1.284
68
-
15 min, in presence of NADH, 80% loss of activity
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.284
5.7
-
reaction with S-formylglutathione and NADH
Homo sapiens
1.1.1.284
6
-
reaction with NADP+ as cofactor
Homo sapiens
1.1.1.284
8
-
reaction with NAD+ as cofactor
Homo sapiens
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.284
NAD+
-
Ovis aries
1.1.1.284
NAD+
-
Bos taurus
1.1.1.284
NAD+
-
Oryctolagus cuniculus
1.1.1.284
NAD+
-
Rattus norvegicus
1.1.1.284
NAD+
-
Homo sapiens
1.1.1.284
NAD+
-
Mus musculus
1.1.1.284
NADH
-
Homo sapiens
1.1.1.284
NADPH
-
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.284
NAD+
-
Ovis aries
1.1.1.284
NAD+
-
Bos taurus
1.1.1.284
NAD+
-
Oryctolagus cuniculus
1.1.1.284
NAD+
-
Rattus norvegicus
1.1.1.284
NAD+
-
Homo sapiens
1.1.1.284
NAD+
-
Mus musculus
1.1.1.284
NADH
-
Homo sapiens
1.1.1.284
NADPH
-
Homo sapiens
General Stability (protein specific)
EC Number
General Stability
Organism
1.1.1.284
one freezing at -20°C results in full inactivation
Homo sapiens
1.1.1.284
overnight dialysis to remove mercaptoethanol destroys 60% of its original activity
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.284
AgNO3
NADH protects more effectively than NAD+
Homo sapiens
1.1.1.284
formaldehyde
-
Homo sapiens
1.1.1.284
Hg2+
NADH protects more effectively than NAD+
Homo sapiens
1.1.1.284
iodoacetate
NADH protects more effectively than NAD+
Homo sapiens
1.1.1.284
methylglyoxal
-
Homo sapiens
1.1.1.284
NAD+
inhibits reverse reaction with NAD+ or NADP+
Homo sapiens
1.1.1.284
NADH
inhibits reaction with formaldehyde, glutathione and NAD+
Homo sapiens
1.1.1.284
NADPH
inhibits reaction with formaldehyde, glutathione and NAD+
Homo sapiens
1.1.1.284
S-formylglutathione
inhibits reaction with formaldehyde, glutathione and NAD+
Homo sapiens
1.1.1.284
S-formylglutathione
product inhibition with S-hydroxymethylglutathione
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.284
0.0042
-
NADH
pH 5.7
Homo sapiens
1.1.1.284
0.0081
-
glutathione
pH 8.0
Homo sapiens
1.1.1.284
0.009
-
NAD+
pH 8.0
Homo sapiens
1.1.1.284
0.0136
-
NAD+
pH 5.7
Homo sapiens
1.1.1.284
0.016
-
NADH
pH 8.0
Homo sapiens
1.1.1.284
0.045
-
NADPH
pH 5.7
Homo sapiens
1.1.1.284
0.28
-
S-formylglutathione
pH 5.7, with NADPH as cofactor
Homo sapiens
1.1.1.284
0.3
-
S-formylglutathione
pH 5.7, with NADH as cofactor
Homo sapiens
1.1.1.284
0.57
-
NADP+
pH 5.7
Homo sapiens
1.1.1.284
4
-
pyruvylglutathione
pH 5.7, with NADH as cofactor
Homo sapiens
1.1.1.284
9.5
-
NADP+
pH 8.0
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.284
cytosol
-
Homo sapiens
5829
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.284
81400
-
gel filtration
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.284
-
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.284
adrenal gland
-
Bos taurus
-
1.1.1.284
ascites tumor cell
-
Mus musculus
-
1.1.1.284
brain
-
Bos taurus
-
1.1.1.284
brain
-
Oryctolagus cuniculus
-
1.1.1.284
brain
-
Rattus norvegicus
-
1.1.1.284
brain
-
Homo sapiens
-
1.1.1.284
cardiac muscle
-
Rattus norvegicus
-
1.1.1.284
kidney
-
Rattus norvegicus
-
1.1.1.284
liver
-
Mus musculus
-
1.1.1.284
liver
-
Ovis aries
-
1.1.1.284
liver
-
Bos taurus
-
1.1.1.284
liver
-
Oryctolagus cuniculus
-
1.1.1.284
liver
-
Rattus norvegicus
-
1.1.1.284
liver
-
Homo sapiens
-
1.1.1.284
skeletal muscle
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.284
3.2
-
-
Homo sapiens
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.1.1.284
-70°C, in presence of 2 mM mercaptoethanol, 50% loss of activity
Homo sapiens
1.1.1.284
0°C, 50% loss of activity after 1 month
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.284
3-ethoxy-2-hydroxybutyraldehyde + glutathione + NAD+
-
287888
Homo sapiens
S-(3-ethoxy-2-hydroxybutyryl)-glutathione + NADH + H+
-
-
-
?
1.1.1.284
formaldehyde + glutathione + NAD+
-
287888
Mus musculus
S-formylglutathione + NADH + H+
-
287888
Mus musculus
-
1.1.1.284
formaldehyde + glutathione + NAD+
-
287888
Homo sapiens
S-formylglutathione + NADH + H+
-
287888
Homo sapiens
r
1.1.1.284
formaldehyde + glutathione + NAD+
-
287888
Rattus norvegicus
S-formylglutathione + NADH + H+
-
287888
Rattus norvegicus
r
1.1.1.284
formaldehyde + glutathione + NAD+
-
287888
Bos taurus
S-formylglutathione + NADH + H+
-
287888
Bos taurus
-
1.1.1.284
formaldehyde + glutathione + NAD+
-
287888
Oryctolagus cuniculus
S-formylglutathione + NADH + H+
-
287888
Oryctolagus cuniculus
-
1.1.1.284
formaldehyde + glutathione + NAD+
-
287888
Ovis aries
S-formylglutathione + NADH + H+
-
287888
Ovis aries
-
1.1.1.284
formaldehyde + glutathione + NADP+
-
287888
Homo sapiens
S-formylglutathione + NADPH + H+
-
287888
Homo sapiens
r
1.1.1.284
formaldehyde + glutathione + NADP+
-
287888
Rattus norvegicus
S-formylglutathione + NADPH + H+
-
287888
Rattus norvegicus
r
1.1.1.284
glyoxal + glutathione + NAD+
-
287888
Homo sapiens
S-oxoacetylglutathione + NADH
-
-
-
?
1.1.1.284
hydroxypyruvaldehyde + glutathione + NAD+
-
287888
Homo sapiens
S-hydroxypyruvylglutathione + NADH
-
-
-
?
1.1.1.284
methylglyoxal + glutathione + NAD+
-
287888
Homo sapiens
S-pyruvylglutathione + NADH
-
-
-
?
1.1.1.284
methylglyoxal + glutathione + NAD+
-
287888
Bos taurus
S-pyruvylglutathione + NADH
-
-
-
?
1.1.1.284
S-formylglutathione + NADH
-
287888
Homo sapiens
formaldehyde + glutathione + NAD+
-
287888
Homo sapiens
r
1.1.1.284
S-formylglutathione + NADH
-
287888
Rattus norvegicus
formaldehyde + glutathione + NAD+
-
287888
Rattus norvegicus
r
1.1.1.284
S-formylglutathione + NADPH
-
287888
Homo sapiens
formaldehyde + glutathione + NADP+
-
287888
Homo sapiens
r
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.284
37
-
15 min, without NADH, 40% loss of activity
Homo sapiens
1.1.1.284
64
-
15 min, in presence of NADH, 40% loss of activity
Homo sapiens
1.1.1.284
68
-
15 min, in presence of NADH, 80% loss of activity
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.284
5.7
-
reaction with S-formylglutathione and NADH
Homo sapiens
1.1.1.284
6
-
reaction with NADP+ as cofactor
Homo sapiens
1.1.1.284
8
-
reaction with NAD+ as cofactor
Homo sapiens