BRENDA - Enzyme Database

Formaldehyde dehydrogenase from formaldehyde-resistant Debaryomyces vanriji FT-1 and Pseudomonas putida F61

Kato, N.; Miyawaki, N.; Sakazawa, C.; Agric. Biol. Chem. 47, 415-416 (1983)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.284
0.15
-
NAD+
-
Pseudomonas putida
1.1.1.284
0.31
-
NAD+
-
Schwanniomyces vanrijiae
1.1.1.284
0.39
-
formaldehyde
-
Pseudomonas putida
1.1.1.284
1.14
-
formaldehyde
-
Schwanniomyces vanrijiae
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.284
40000
-
2 * 40000, SDS-PAGE
Schwanniomyces vanrijiae
1.1.1.284
42000
-
2 * 42000, SDS-PAGE
Pseudomonas putida
1.1.1.284
72000
-
gel filtration
Pseudomonas putida
1.1.1.284
72000
-
gel filtration
Schwanniomyces vanrijiae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.284
Pseudomonas putida
-
-
-
1.1.1.284
Pseudomonas putida F61
-
-
-
1.1.1.284
Schwanniomyces vanrijiae
-
-
-
1.1.1.284
Schwanniomyces vanrijiae FT-1
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.284
-
Pseudomonas putida
1.1.1.284
-
Schwanniomyces vanrijiae
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.284
184
-
-
Schwanniomyces vanrijiae
1.1.1.284
185
-
-
Pseudomonas putida
Storage Stability
EC Number
Storage Stability
Organism
1.1.1.284
5°C, 10 mM potassium phosphate buffer, pH 7.5, 0.1% 2-mercaptoethanol, stable for at least 1 month
Pseudomonas putida
1.1.1.284
5°C, 10 mM potassium phosphate buffer, pH 7.5, 0.1% 2-mercaptoethanol, stable for at least 1 month
Schwanniomyces vanrijiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.284
formaldehyde + glutathione + NAD+
-
287885
Pseudomonas putida
S-formylglutathione + NADH + H+
-
287885
Pseudomonas putida
?
1.1.1.284
formaldehyde + glutathione + NAD+
-
287885
Schwanniomyces vanrijiae
S-formylglutathione + NADH + H+
-
287885
Schwanniomyces vanrijiae
?
1.1.1.284
formaldehyde + glutathione + NAD+
-
287885
Pseudomonas putida F61
S-formylglutathione + NADH + H+
-
287885
Pseudomonas putida F61
?
1.1.1.284
formaldehyde + glutathione + NAD+
-
287885
Schwanniomyces vanrijiae FT-1
S-formylglutathione + NADH + H+
-
287885
Schwanniomyces vanrijiae FT-1
?
1.1.1.284
methylglyoxal + glutathione + NAD+
-
287885
Pseudomonas putida
S-pyruvylglutathione + NADH
-
-
-
?
1.1.1.284
methylglyoxal + glutathione + NAD+
-
287885
Schwanniomyces vanrijiae
S-pyruvylglutathione + NADH
-
-
-
?
1.1.1.284
methylglyoxal + glutathione + NAD+
-
287885
Pseudomonas putida F61
S-pyruvylglutathione + NADH
-
-
-
?
1.1.1.284
methylglyoxal + glutathione + NAD+
-
287885
Schwanniomyces vanrijiae FT-1
S-pyruvylglutathione + NADH
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.284
dimer
2 * 42000, SDS-PAGE
Pseudomonas putida
1.1.1.284
dimer
2 * 40000, SDS-PAGE
Schwanniomyces vanrijiae
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.284
35
-
-
Schwanniomyces vanrijiae
1.1.1.284
45
-
-
Pseudomonas putida
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.284
30
-
10 min, stable
Schwanniomyces vanrijiae
1.1.1.284
40
-
10 min, stable
Pseudomonas putida
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.284
8
-
-
Schwanniomyces vanrijiae
1.1.1.284
9
-
-
Pseudomonas putida
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.1.1.284
6
8.5
4°C, 3 d, stable
Pseudomonas putida
1.1.1.284
6.5
8.5
4°C, 3 d, stable
Schwanniomyces vanrijiae
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.284
NAD+
-
Pseudomonas putida
1.1.1.284
NAD+
-
Schwanniomyces vanrijiae
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.284
NAD+
-
Pseudomonas putida
1.1.1.284
NAD+
-
Schwanniomyces vanrijiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.284
0.15
-
NAD+
-
Pseudomonas putida
1.1.1.284
0.31
-
NAD+
-
Schwanniomyces vanrijiae
1.1.1.284
0.39
-
formaldehyde
-
Pseudomonas putida
1.1.1.284
1.14
-
formaldehyde
-
Schwanniomyces vanrijiae
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.284
40000
-
2 * 40000, SDS-PAGE
Schwanniomyces vanrijiae
1.1.1.284
42000
-
2 * 42000, SDS-PAGE
Pseudomonas putida
1.1.1.284
72000
-
gel filtration
Pseudomonas putida
1.1.1.284
72000
-
gel filtration
Schwanniomyces vanrijiae
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.284
-
Pseudomonas putida
1.1.1.284
-
Schwanniomyces vanrijiae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.284
184
-
-
Schwanniomyces vanrijiae
1.1.1.284
185
-
-
Pseudomonas putida
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.1.1.284
5°C, 10 mM potassium phosphate buffer, pH 7.5, 0.1% 2-mercaptoethanol, stable for at least 1 month
Pseudomonas putida
1.1.1.284
5°C, 10 mM potassium phosphate buffer, pH 7.5, 0.1% 2-mercaptoethanol, stable for at least 1 month
Schwanniomyces vanrijiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.284
formaldehyde + glutathione + NAD+
-
287885
Pseudomonas putida
S-formylglutathione + NADH + H+
-
287885
Pseudomonas putida
?
1.1.1.284
formaldehyde + glutathione + NAD+
-
287885
Schwanniomyces vanrijiae
S-formylglutathione + NADH + H+
-
287885
Schwanniomyces vanrijiae
?
1.1.1.284
formaldehyde + glutathione + NAD+
-
287885
Pseudomonas putida F61
S-formylglutathione + NADH + H+
-
287885
Pseudomonas putida F61
?
1.1.1.284
formaldehyde + glutathione + NAD+
-
287885
Schwanniomyces vanrijiae FT-1
S-formylglutathione + NADH + H+
-
287885
Schwanniomyces vanrijiae FT-1
?
1.1.1.284
methylglyoxal + glutathione + NAD+
-
287885
Pseudomonas putida
S-pyruvylglutathione + NADH
-
-
-
?
1.1.1.284
methylglyoxal + glutathione + NAD+
-
287885
Schwanniomyces vanrijiae
S-pyruvylglutathione + NADH
-
-
-
?
1.1.1.284
methylglyoxal + glutathione + NAD+
-
287885
Pseudomonas putida F61
S-pyruvylglutathione + NADH
-
-
-
?
1.1.1.284
methylglyoxal + glutathione + NAD+
-
287885
Schwanniomyces vanrijiae FT-1
S-pyruvylglutathione + NADH
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.284
dimer
2 * 42000, SDS-PAGE
Pseudomonas putida
1.1.1.284
dimer
2 * 40000, SDS-PAGE
Schwanniomyces vanrijiae
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.284
35
-
-
Schwanniomyces vanrijiae
1.1.1.284
45
-
-
Pseudomonas putida
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.284
30
-
10 min, stable
Schwanniomyces vanrijiae
1.1.1.284
40
-
10 min, stable
Pseudomonas putida
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.284
8
-
-
Schwanniomyces vanrijiae
1.1.1.284
9
-
-
Pseudomonas putida
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.1.1.284
6
8.5
4°C, 3 d, stable
Pseudomonas putida
1.1.1.284
6.5
8.5
4°C, 3 d, stable
Schwanniomyces vanrijiae