BRENDA - Enzyme Database

Microbial assimilation of methanol. Properties of formaldehyde dehydrogenase and formate dehydrogenase from Candida boidinii

Sahm, H.; Wagner, F.; Arch. Mikrobiol. 90, 263-268 (1973)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.284
0.05
-
NAD+
-
Candida boidinii
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.284
formaldehyde + glutathione + NAD+
Candida boidinii
the synthesis of the enzyme is induced by methanol and repressed by glucose
S-formylglutathione + NADH + H+
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.284
Candida boidinii
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.284
partial
Candida boidinii
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.284
8.7
-
-
Candida boidinii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.284
formaldehyde + glutathione + NAD+
-
287879
Candida boidinii
S-formylglutathione + NADH + H+
-
287879
Candida boidinii
?
1.1.1.284
formaldehyde + glutathione + NAD+
the synthesis of the enzyme is induced by methanol and repressed by glucose
287879
Candida boidinii
S-formylglutathione + NADH + H+
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.284
8
-
-
Candida boidinii
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.284
NAD+
-
Candida boidinii
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.284
NAD+
-
Candida boidinii
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.284
0.05
-
NAD+
-
Candida boidinii
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.284
formaldehyde + glutathione + NAD+
Candida boidinii
the synthesis of the enzyme is induced by methanol and repressed by glucose
S-formylglutathione + NADH + H+
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.284
partial
Candida boidinii
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.284
8.7
-
-
Candida boidinii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.284
formaldehyde + glutathione + NAD+
-
287879
Candida boidinii
S-formylglutathione + NADH + H+
-
287879
Candida boidinii
?
1.1.1.284
formaldehyde + glutathione + NAD+
the synthesis of the enzyme is induced by methanol and repressed by glucose
287879
Candida boidinii
S-formylglutathione + NADH + H+
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.284
8
-
-
Candida boidinii