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Literature summary extracted from

  • Sahm, H.; Wagner, F.
    Microbial assimilation of methanol. Properties of formaldehyde dehydrogenase and formate dehydrogenase from Candida boidinii (1973), Arch. Mikrobiol., 90, 263-268.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.284 0.05
-
NAD+
-
[Candida] boidinii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.284 formaldehyde + glutathione + NAD+ [Candida] boidinii the synthesis of the enzyme is induced by methanol and repressed by glucose S-formylglutathione + NADH + H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.284 [Candida] boidinii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.284 partial [Candida] boidinii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.284 8.7
-
-
[Candida] boidinii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.284 formaldehyde + glutathione + NAD+
-
[Candida] boidinii S-formylglutathione + NADH + H+
-
?
1.1.1.284 formaldehyde + glutathione + NAD+ the synthesis of the enzyme is induced by methanol and repressed by glucose [Candida] boidinii S-formylglutathione + NADH + H+
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.284 8
-
-
[Candida] boidinii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.284 NAD+
-
[Candida] boidinii