BRENDA - Enzyme Database

A steady-state-kinetic model for formaldehyde dehydrogenase from human liver. A mechanism involving NAD+ and the hemimercaptal adduct of glutathione and formaldehyde as substrates and free glutathione as an allosteric activator of the enzyme

Uotila, L.; Mannervik, B.; Biochem. J. 177, 869-878 (1979)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.284
ADP-ribose
competitive with respect to beta-NAD+ and non-competitive with glutathione
Homo sapiens
1.1.1.284
Alpha-NAD+
competitive with respect to beta-NAD+ and non-competitive with glutathione
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.284
0.0048
-
beta-NAD+
-
Homo sapiens
1.1.1.284
0.0301
-
thio-NAD+
-
Homo sapiens
1.1.1.284
0.0352
-
nicotinamide-hypoxanthine dinucleotide
-
Homo sapiens
1.1.1.284
0.0509
-
3-acetylpyridine-adenine dinucleotide
-
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.284
Homo sapiens
-
-
-
Reaction
EC Number
Reaction
Commentary
Organism
1.1.1.284
S-(hydroxymethyl)glutathione + NAD(P)+ = S-formylglutathione + NAD(P)H + H+
random sequential mechanism
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.284
liver
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.284
formaldehyde + glutathione + 3-acetylpyridine-adenine dinucleotide
-
287874
Homo sapiens
S-formylglutathione + ?
-
-
-
?
1.1.1.284
formaldehyde + glutathione + NAD+
-
287874
Homo sapiens
S-formylglutathione + NADH + H+
-
287874
Homo sapiens
?
1.1.1.284
formaldehyde + glutathione + nicotinamide-hypoxanthine dinucleotide
-
287874
Homo sapiens
S-formylglutathione + ?
-
-
-
?
1.1.1.284
formaldehyde + glutathione + thio-NAD+
-
287874
Homo sapiens
S-formylglutathione + thio-NADH
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.284
3-acetylpyridine adenine dinucleotide
can substitute for beta-NAD+
Homo sapiens
1.1.1.284
NAD+
-
Homo sapiens
1.1.1.284
nicotinamide hypoxanthine dinucleotide
can substitute for beta-NAD+
Homo sapiens
1.1.1.284
thio-NAD+
can substitute for beta-NAD+
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.284
3-acetylpyridine adenine dinucleotide
can substitute for beta-NAD+
Homo sapiens
1.1.1.284
NAD+
-
Homo sapiens
1.1.1.284
nicotinamide hypoxanthine dinucleotide
can substitute for beta-NAD+
Homo sapiens
1.1.1.284
thio-NAD+
can substitute for beta-NAD+
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.284
ADP-ribose
competitive with respect to beta-NAD+ and non-competitive with glutathione
Homo sapiens
1.1.1.284
Alpha-NAD+
competitive with respect to beta-NAD+ and non-competitive with glutathione
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.284
0.0048
-
beta-NAD+
-
Homo sapiens
1.1.1.284
0.0301
-
thio-NAD+
-
Homo sapiens
1.1.1.284
0.0352
-
nicotinamide-hypoxanthine dinucleotide
-
Homo sapiens
1.1.1.284
0.0509
-
3-acetylpyridine-adenine dinucleotide
-
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.284
liver
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.284
formaldehyde + glutathione + 3-acetylpyridine-adenine dinucleotide
-
287874
Homo sapiens
S-formylglutathione + ?
-
-
-
?
1.1.1.284
formaldehyde + glutathione + NAD+
-
287874
Homo sapiens
S-formylglutathione + NADH + H+
-
287874
Homo sapiens
?
1.1.1.284
formaldehyde + glutathione + nicotinamide-hypoxanthine dinucleotide
-
287874
Homo sapiens
S-formylglutathione + ?
-
-
-
?
1.1.1.284
formaldehyde + glutathione + thio-NAD+
-
287874
Homo sapiens
S-formylglutathione + thio-NADH
-
-
-
?