BRENDA - Enzyme Database

A comparison of rat and human liver formaldehyde dehydrogenase

Goodman, J.I.; Tephly, T.R.; Biochim. Biophys. Acta 252, 489-505 (1971)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.1.284
pyrazole
no effect
Homo sapiens
1.1.1.284
pyrazole
stimulates
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.284
folic acid
-
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.284
0.007
-
NAD+
-
Homo sapiens
1.1.1.284
0.0071
-
formaldehyde
-
Rattus norvegicus
1.1.1.284
0.0087
-
formaldehyde
-
Homo sapiens
1.1.1.284
0.01
-
NAD+
-
Rattus norvegicus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.284
additional information
contains metal
Homo sapiens
1.1.1.284
additional information
does not contain metal
Rattus norvegicus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.284
90000
-
gel filtration
Homo sapiens
1.1.1.284
110000
-
gel filtration
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.284
Homo sapiens
-
-
-
1.1.1.284
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.284
-
Rattus norvegicus
1.1.1.284
-
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.284
liver
-
Rattus norvegicus
-
1.1.1.284
liver
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.284
0.092
-
-
Rattus norvegicus
1.1.1.284
0.225
-
-
Homo sapiens
Storage Stability
EC Number
Storage Stability
Organism
1.1.1.284
0-5°C, when EDTA and dithiothreitol are used throughout the purification procedure, stable for at least 3 weeks
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.284
formaldehyde + glutathione + NAD+
-
287866
Homo sapiens
S-formylglutathione + NADH + H+
-
287866
Homo sapiens
?
1.1.1.284
formaldehyde + glutathione + NAD+
-
287866
Rattus norvegicus
S-formylglutathione + NADH + H+
-
287866
Rattus norvegicus
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.284
More
the enzyme exists in a complex with other proteins or in a polymeric form until the ultimate steps in purification
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.284
7.5
8.4
-
Rattus norvegicus
1.1.1.284
7.6
8.8
-
Homo sapiens
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.284
6.5
9.5
pH 6.5: about 70% of maximal activity, pH 9.5: about 80% of maximal activity
Homo sapiens
1.1.1.284
6.8
9.3
pH 6.8: about 80% of maximal activity, pH 9.3: about 90% of maximal activity
Rattus norvegicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.284
NAD+
-
Rattus norvegicus
1.1.1.284
NAD+
-
Homo sapiens
1.1.1.284
NADP+
no activity with NADP+
Homo sapiens
1.1.1.284
NADP+
no activity with NADP+
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.1.284
pyrazole
no effect
Homo sapiens
1.1.1.284
pyrazole
stimulates
Rattus norvegicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.284
NAD+
-
Rattus norvegicus
1.1.1.284
NAD+
-
Homo sapiens
1.1.1.284
NADP+
no activity with NADP+
Homo sapiens
1.1.1.284
NADP+
no activity with NADP+
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.284
folic acid
-
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.284
0.007
-
NAD+
-
Homo sapiens
1.1.1.284
0.0071
-
formaldehyde
-
Rattus norvegicus
1.1.1.284
0.0087
-
formaldehyde
-
Homo sapiens
1.1.1.284
0.01
-
NAD+
-
Rattus norvegicus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.284
additional information
contains metal
Homo sapiens
1.1.1.284
additional information
does not contain metal
Rattus norvegicus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.284
90000
-
gel filtration
Homo sapiens
1.1.1.284
110000
-
gel filtration
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.284
-
Rattus norvegicus
1.1.1.284
-
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.284
liver
-
Rattus norvegicus
-
1.1.1.284
liver
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.284
0.092
-
-
Rattus norvegicus
1.1.1.284
0.225
-
-
Homo sapiens
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.1.1.284
0-5°C, when EDTA and dithiothreitol are used throughout the purification procedure, stable for at least 3 weeks
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.284
formaldehyde + glutathione + NAD+
-
287866
Homo sapiens
S-formylglutathione + NADH + H+
-
287866
Homo sapiens
?
1.1.1.284
formaldehyde + glutathione + NAD+
-
287866
Rattus norvegicus
S-formylglutathione + NADH + H+
-
287866
Rattus norvegicus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.284
More
the enzyme exists in a complex with other proteins or in a polymeric form until the ultimate steps in purification
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.284
7.5
8.4
-
Rattus norvegicus
1.1.1.284
7.6
8.8
-
Homo sapiens
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.284
6.5
9.5
pH 6.5: about 70% of maximal activity, pH 9.5: about 80% of maximal activity
Homo sapiens
1.1.1.284
6.8
9.3
pH 6.8: about 80% of maximal activity, pH 9.3: about 90% of maximal activity
Rattus norvegicus