BRENDA - Enzyme Database

Residues specific for class III alcohol dehydrogenase. Site-directed mutagenesis of the human enzyme

Estonius, M.; Hoeoeg, J.O.; Danielsson, O.; Joernvall, H.; Biochemistry 33, 15080-15085 (1994)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.284
-
Homo sapiens
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.1.1.284
D57L
considerable loss of formaldehyde dehydrogenase activity
Homo sapiens
1.1.1.284
D57L/Y93F
fall in ratio kcat/Km for hydroxymethylglutathione by a factor of 1250, alcohol dehydrogenase activity of the mutant has gained a characteristic class I property, complete inhibition by 4-methylpyrazole at concentrations only partially reducing the activity of the wild-type class III enzyme
Homo sapiens
1.1.1.284
T48A
enzyme has essentially no alcohol dehydrogenase activity but has some glutathione-dependent formaldehyde dehydrogenase activity
Homo sapiens
1.1.1.284
Y93F
decreased turnover number for substrates in general, inhibition of alcohol dehydrogenase activity by 4-methylpyrazole, which is not found in the wild-type enzyme
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.284
Homo sapiens
-
class II alcohol dehydrogenase
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.284
wild-type recombinant enzyme
Homo sapiens
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.284
additional information
-
-
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.284
formaldehyde + glutathione + NAD+
-
287863
Homo sapiens
S-formylglutathione + NADH + H+
-
287863
Homo sapiens
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.284
NAD+
-
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.284
-
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.284
NAD+
-
Homo sapiens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.1.1.284
D57L
considerable loss of formaldehyde dehydrogenase activity
Homo sapiens
1.1.1.284
D57L/Y93F
fall in ratio kcat/Km for hydroxymethylglutathione by a factor of 1250, alcohol dehydrogenase activity of the mutant has gained a characteristic class I property, complete inhibition by 4-methylpyrazole at concentrations only partially reducing the activity of the wild-type class III enzyme
Homo sapiens
1.1.1.284
T48A
enzyme has essentially no alcohol dehydrogenase activity but has some glutathione-dependent formaldehyde dehydrogenase activity
Homo sapiens
1.1.1.284
Y93F
decreased turnover number for substrates in general, inhibition of alcohol dehydrogenase activity by 4-methylpyrazole, which is not found in the wild-type enzyme
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.284
wild-type recombinant enzyme
Homo sapiens
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.284
additional information
-
-
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.284
formaldehyde + glutathione + NAD+
-
287863
Homo sapiens
S-formylglutathione + NADH + H+
-
287863
Homo sapiens
?