EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.284 | - |
Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.284 | D57L | considerable loss of formaldehyde dehydrogenase activity | Homo sapiens |
1.1.1.284 | D57L/Y93F | fall in ratio kcat/Km for hydroxymethylglutathione by a factor of 1250, alcohol dehydrogenase activity of the mutant has gained a characteristic class I property, complete inhibition by 4-methylpyrazole at concentrations only partially reducing the activity of the wild-type class III enzyme | Homo sapiens |
1.1.1.284 | T48A | enzyme has essentially no alcohol dehydrogenase activity but has some glutathione-dependent formaldehyde dehydrogenase activity | Homo sapiens |
1.1.1.284 | Y93F | decreased turnover number for substrates in general, inhibition of alcohol dehydrogenase activity by 4-methylpyrazole, which is not found in the wild-type enzyme | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.284 | Homo sapiens | - |
class II alcohol dehydrogenase | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.284 | wild-type recombinant enzyme | Homo sapiens |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.284 | additional information | - |
- |
Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.284 | formaldehyde + glutathione + NAD+ | - |
Homo sapiens | S-formylglutathione + NADH + H+ | - |
? |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.284 | NAD+ | - |
Homo sapiens |