BRENDA - Enzyme Database

Formaldehyde dehydrogenase from methylotrophic yeasts

Kato, N.; Methods Enzymol. 188, 455-462 (1990)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.284
ADP
-
Candida boidinii
1.1.1.284
Ag+
-
Candida boidinii
1.1.1.284
AMP
-
Candida boidinii
1.1.1.284
ATP
-
Candida boidinii
1.1.1.284
Cd2+
-
Komagataella pastoris
1.1.1.284
Cd2+
-
Candida boidinii
1.1.1.284
Cu2+
-
Komagataella pastoris
1.1.1.284
Cu2+
-
Candida boidinii
1.1.1.284
Hg2+
-
Komagataella pastoris
1.1.1.284
Hg2+
-
Candida boidinii
1.1.1.284
NADH
competitive inhibition, product inhibition
Candida boidinii
1.1.1.284
NADH
-
Komagataella pastoris
1.1.1.284
PCMB
-
Komagataella pastoris
1.1.1.284
PCMB
-
Candida boidinii
1.1.1.284
S-formylglutathione
-
Candida boidinii
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.284
0.025
-
NAD+
-
Candida boidinii
1.1.1.284
0.025
-
NADH
-
Candida boidinii
1.1.1.284
0.12
-
S-formylglutathione
-
Candida boidinii
1.1.1.284
0.29
-
formaldehyde
-
Candida boidinii
1.1.1.284
2.8
-
methylglyoxal
-
Candida boidinii
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.284
43000
-
2 * 43000, SDS-PAGE
Candida boidinii
1.1.1.284
82000
-
gel filtration
Candida boidinii
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.284
formaldehyde + glutathione + NAD+
Ogataea angusta
-
S-formylglutathione + NADH + H+
-
-
?
1.1.1.284
formaldehyde + glutathione + NAD+
Candida boidinii
key step of the methanol catabolism in yeast
S-formylglutathione + NADH + H+
-
-
?
1.1.1.284
formaldehyde + glutathione + NAD+
Candida boidinii 2201
key step of the methanol catabolism in yeast
S-formylglutathione + NADH + H+
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.284
Candida boidinii
-
-
-
1.1.1.284
Candida boidinii 2201
-
-
-
1.1.1.284
Komagataella pastoris
-
-
-
1.1.1.284
Ogataea angusta
-
-
-
1.1.1.284
Pichia sp.
-
NRRL-Y-11328
-
1.1.1.284
Pichia sp. NRRL-Y-11328
-
NRRL-Y-11328
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.284
-
Candida boidinii
Reaction
EC Number
Reaction
Commentary
Organism
1.1.1.284
S-(hydroxymethyl)glutathione + NAD(P)+ = S-formylglutathione + NAD(P)H + H+
bi bi mechanism
Candida boidinii
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.284
38.3
-
-
Candida boidinii
Storage Stability
EC Number
Storage Stability
Organism
1.1.1.284
-20°C, in presence of glycerol, stable for at least 6 months
Candida boidinii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.284
formaldehyde + glutathione + NAD+
-
287862
Komagataella pastoris
S-formylglutathione + NADH + H+
-
287862
Komagataella pastoris
r
1.1.1.284
formaldehyde + glutathione + NAD+
-
287862
Ogataea angusta
S-formylglutathione + NADH + H+
-
287862
Ogataea angusta
-
1.1.1.284
formaldehyde + glutathione + NAD+
-
287862
Ogataea angusta
S-formylglutathione + NADH + H+
-
-
-
?
1.1.1.284
formaldehyde + glutathione + NAD+
-
287862
Pichia sp.
S-formylglutathione + NADH + H+
-
287862
Pichia sp.
-
1.1.1.284
formaldehyde + glutathione + NAD+
the true substrate is a hemimercaptal, S-hydroxymethylglutathione, spontaneously formed from formaldehyde and glutathione
287862
Candida boidinii
S-formylglutathione + NADH + H+
-
287862
Candida boidinii
r
1.1.1.284
formaldehyde + glutathione + NAD+
key step of the methanol catabolism in yeast
287862
Candida boidinii
S-formylglutathione + NADH + H+
-
-
-
?
1.1.1.284
formaldehyde + glutathione + NAD+
the true substrate is a hemimercaptal, S-hydroxymethylglutathione, spontaneously formed from formaldehyde and glutathione
287862
Candida boidinii 2201
S-formylglutathione + NADH + H+
-
287862
Candida boidinii 2201
r
1.1.1.284
formaldehyde + glutathione + NAD+
key step of the methanol catabolism in yeast
287862
Candida boidinii 2201
S-formylglutathione + NADH + H+
-
-
-
?
1.1.1.284
formaldehyde + glutathione + NAD+
-
287862
Pichia sp. NRRL-Y-11328
S-formylglutathione + NADH + H+
-
287862
Pichia sp. NRRL-Y-11328
-
1.1.1.284
methylglyoxal + glutathione + NAD+
-
287862
Komagataella pastoris
S-pyruvylglutathione + NADH
-
-
-
?
1.1.1.284
methylglyoxal + glutathione + NAD+
89% of the activity with formaldehyde
287862
Candida boidinii
S-pyruvylglutathione + NADH
-
-
-
?
1.1.1.284
methylglyoxal + glutathione + NAD+
89% of the activity with formaldehyde
287862
Candida boidinii 2201
S-pyruvylglutathione + NADH
-
-
-
?
1.1.1.284
S-formylglutathione + NADH
-
287862
Komagataella pastoris
formaldehyde + glutathione + NAD+
-
287862
Komagataella pastoris
r
1.1.1.284
S-formylglutathione + NADH
-
287862
Candida boidinii
formaldehyde + glutathione + NAD+
-
287862
Candida boidinii
r
1.1.1.284
S-formylglutathione + NADH
-
287862
Candida boidinii 2201
formaldehyde + glutathione + NAD+
-
287862
Candida boidinii 2201
r
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.284
dimer
2 * 43000, SDS-PAGE
Candida boidinii
1.1.1.284
dimer
-
Komagataella pastoris
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.284
8
-
-
Candida boidinii
1.1.1.284
8
-
-
Komagataella pastoris
1.1.1.284
8
-
-
Ogataea angusta
1.1.1.284
8.5
-
-
Candida boidinii
1.1.1.284
8.5
-
-
Komagataella pastoris
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.1.1.284
6
10
20°C, 1 h, stable
Candida boidinii
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.284
NAD+
-
Pichia sp.
1.1.1.284
NAD+
-
Ogataea angusta
1.1.1.284
NAD+
-
Candida boidinii
1.1.1.284
NAD+
-
Komagataella pastoris
1.1.1.284
NADH
-
Komagataella pastoris
1.1.1.284
NADH
-
Candida boidinii
1.1.1.284
NADP+
no activity with NADP+
Komagataella pastoris
1.1.1.284
NADP+
no activity with NADP+
Candida boidinii
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.284
NAD+
-
Pichia sp.
1.1.1.284
NAD+
-
Ogataea angusta
1.1.1.284
NAD+
-
Candida boidinii
1.1.1.284
NAD+
-
Komagataella pastoris
1.1.1.284
NADH
-
Komagataella pastoris
1.1.1.284
NADH
-
Candida boidinii
1.1.1.284
NADP+
no activity with NADP+
Komagataella pastoris
1.1.1.284
NADP+
no activity with NADP+
Candida boidinii
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.284
ADP
-
Candida boidinii
1.1.1.284
Ag+
-
Candida boidinii
1.1.1.284
AMP
-
Candida boidinii
1.1.1.284
ATP
-
Candida boidinii
1.1.1.284
Cd2+
-
Komagataella pastoris
1.1.1.284
Cd2+
-
Candida boidinii
1.1.1.284
Cu2+
-
Komagataella pastoris
1.1.1.284
Cu2+
-
Candida boidinii
1.1.1.284
Hg2+
-
Komagataella pastoris
1.1.1.284
Hg2+
-
Candida boidinii
1.1.1.284
NADH
competitive inhibition, product inhibition
Candida boidinii
1.1.1.284
NADH
-
Komagataella pastoris
1.1.1.284
PCMB
-
Komagataella pastoris
1.1.1.284
PCMB
-
Candida boidinii
1.1.1.284
S-formylglutathione
-
Candida boidinii
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.284
0.025
-
NAD+
-
Candida boidinii
1.1.1.284
0.025
-
NADH
-
Candida boidinii
1.1.1.284
0.12
-
S-formylglutathione
-
Candida boidinii
1.1.1.284
0.29
-
formaldehyde
-
Candida boidinii
1.1.1.284
2.8
-
methylglyoxal
-
Candida boidinii
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.284
43000
-
2 * 43000, SDS-PAGE
Candida boidinii
1.1.1.284
82000
-
gel filtration
Candida boidinii
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.284
formaldehyde + glutathione + NAD+
Ogataea angusta
-
S-formylglutathione + NADH + H+
-
-
?
1.1.1.284
formaldehyde + glutathione + NAD+
Candida boidinii
key step of the methanol catabolism in yeast
S-formylglutathione + NADH + H+
-
-
?
1.1.1.284
formaldehyde + glutathione + NAD+
Candida boidinii 2201
key step of the methanol catabolism in yeast
S-formylglutathione + NADH + H+
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.284
-
Candida boidinii
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.284
38.3
-
-
Candida boidinii
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.1.1.284
-20°C, in presence of glycerol, stable for at least 6 months
Candida boidinii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.284
formaldehyde + glutathione + NAD+
-
287862
Komagataella pastoris
S-formylglutathione + NADH + H+
-
287862
Komagataella pastoris
r
1.1.1.284
formaldehyde + glutathione + NAD+
-
287862
Ogataea angusta
S-formylglutathione + NADH + H+
-
287862
Ogataea angusta
-
1.1.1.284
formaldehyde + glutathione + NAD+
-
287862
Ogataea angusta
S-formylglutathione + NADH + H+
-
-
-
?
1.1.1.284
formaldehyde + glutathione + NAD+
-
287862
Pichia sp.
S-formylglutathione + NADH + H+
-
287862
Pichia sp.
-
1.1.1.284
formaldehyde + glutathione + NAD+
the true substrate is a hemimercaptal, S-hydroxymethylglutathione, spontaneously formed from formaldehyde and glutathione
287862
Candida boidinii
S-formylglutathione + NADH + H+
-
287862
Candida boidinii
r
1.1.1.284
formaldehyde + glutathione + NAD+
key step of the methanol catabolism in yeast
287862
Candida boidinii
S-formylglutathione + NADH + H+
-
-
-
?
1.1.1.284
formaldehyde + glutathione + NAD+
the true substrate is a hemimercaptal, S-hydroxymethylglutathione, spontaneously formed from formaldehyde and glutathione
287862
Candida boidinii 2201
S-formylglutathione + NADH + H+
-
287862
Candida boidinii 2201
r
1.1.1.284
formaldehyde + glutathione + NAD+
key step of the methanol catabolism in yeast
287862
Candida boidinii 2201
S-formylglutathione + NADH + H+
-
-
-
?
1.1.1.284
formaldehyde + glutathione + NAD+
-
287862
Pichia sp. NRRL-Y-11328
S-formylglutathione + NADH + H+
-
287862
Pichia sp. NRRL-Y-11328
-
1.1.1.284
methylglyoxal + glutathione + NAD+
-
287862
Komagataella pastoris
S-pyruvylglutathione + NADH
-
-
-
?
1.1.1.284
methylglyoxal + glutathione + NAD+
89% of the activity with formaldehyde
287862
Candida boidinii
S-pyruvylglutathione + NADH
-
-
-
?
1.1.1.284
methylglyoxal + glutathione + NAD+
89% of the activity with formaldehyde
287862
Candida boidinii 2201
S-pyruvylglutathione + NADH
-
-
-
?
1.1.1.284
S-formylglutathione + NADH
-
287862
Komagataella pastoris
formaldehyde + glutathione + NAD+
-
287862
Komagataella pastoris
r
1.1.1.284
S-formylglutathione + NADH
-
287862
Candida boidinii
formaldehyde + glutathione + NAD+
-
287862
Candida boidinii
r
1.1.1.284
S-formylglutathione + NADH
-
287862
Candida boidinii 2201
formaldehyde + glutathione + NAD+
-
287862
Candida boidinii 2201
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.284
dimer
2 * 43000, SDS-PAGE
Candida boidinii
1.1.1.284
dimer
-
Komagataella pastoris
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.284
8
-
-
Candida boidinii
1.1.1.284
8
-
-
Komagataella pastoris
1.1.1.284
8
-
-
Ogataea angusta
1.1.1.284
8.5
-
-
Candida boidinii
1.1.1.284
8.5
-
-
Komagataella pastoris
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.1.1.284
6
10
20°C, 1 h, stable
Candida boidinii