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Literature summary extracted from

  • Vrtis, J.M.; White, A.K.; Metcalf, W.W.; van der Donk, W.A.
    Phosphite dehydrogenase: An unusual phosphoryl transfer reaction (2001), J. Am. Chem. Soc., 123, 2672-2673.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.20.1.1 in Escherichia coli Stutzerimonas stutzeri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.20.1.1 phosphite + H2O + NAD+ Stutzerimonas stutzeri
-
 phosphate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.20.1.1 Stutzerimonas stutzeri
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.20.1.1 recombinant and native protein Stutzerimonas stutzeri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.20.1.1 0.01
-
 crude extract from Pseudomonas stutzeri Stutzerimonas stutzeri
1.20.1.1 0.21
-
 crude extract from Escherichia coli clone Stutzerimonas stutzeri
1.20.1.1 5.2
-
 purified enzyme from Pseudomonas stutzeri Stutzerimonas stutzeri
1.20.1.1 6.52
-
 purified enzyme from Escherichia coli clone Stutzerimonas stutzeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.20.1.1 phosphite + H2O + NAD+
-
 Stutzerimonas stutzeri phosphate + NADH + H+
-
 ?
1.20.1.1 phosphite + H2O + NADP+ NADP poorly substitutes for NAD+ Stutzerimonas stutzeri phosphate + NADPH + H+
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.20.1.1 35
-
-
 Stutzerimonas stutzeri

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.20.1.1 15 50 25% of activity at 15°C, no activity at 50°C Stutzerimonas stutzeri