Data extracted from this reference:
Cloned(Commentary)
1.20.1.1
in Escherichia coli
Pseudomonas stutzeri
Inhibitors
1.20.1.1
arsenite
Pseudomonas stutzeri
1.20.1.1
D,L-hydroxyisocaproate
Pseudomonas stutzeri
1.20.1.1
D-2-hydroxy-4-methylvalerate
Pseudomonas stutzeri
1.20.1.1
D-glycerate
Pseudomonas stutzeri
1.20.1.1
formate
Pseudomonas stutzeri
1.20.1.1
NaCl
Pseudomonas stutzeri
1.20.1.1
NADH
competitive
Pseudomonas stutzeri
1.20.1.1
nitrite
Pseudomonas stutzeri
1.20.1.1
sulfite
dead end inhibitor, competitive
Pseudomonas stutzeri
KM Value [mM]
1.20.1.1
0.053
phosphite
Pseudomonas stutzeri
1.20.1.1
0.055
NAD+
Pseudomonas stutzeri
Molecular Weight [Da]
1.20.1.1
69000
gel filtration
Pseudomonas stutzeri
Natural Substrates/ Products (Substrates)
1.20.1.1
phosphite + H2O + NAD+
Pseudomonas stutzeri
use of phosphite as energy source, regeneration of NADH
phosphate + NADH + H+
Pseudomonas stutzeri
ir
Organism
1.20.1.1
Pseudomonas stutzeri
O69054
Purification (Commentary)
1.20.1.1
recombinant protein
Pseudomonas stutzeri
Specific Activity [micromol/min/mg]
1.20.1.1
12.2
Pseudomonas stutzeri
Substrates and Products (Substrate)
1.20.1.1
hydroxypyruvate + NAD+
very poor substrate
287853
Pseudomonas stutzeri
pyruvate + NADH
287853
Pseudomonas stutzeri
?
1.20.1.1
phosphite + H2O + NAD+
287853
Pseudomonas stutzeri
phosphate + NADH + H+
287853
Pseudomonas stutzeri
ir
1.20.1.1
phosphite + H2O + NAD+
use of phosphite as energy source, regeneration of NADH
287853
Pseudomonas stutzeri
phosphate + NADH + H+
287853
Pseudomonas stutzeri
ir
1.20.1.1
phosphite + H2O + NADP+
NADP poorly substitutes for NAD+
287853
Pseudomonas stutzeri
phosphate + NADPH + H+
287853
Pseudomonas stutzeri
?
Subunits
1.20.1.1
homodimer
gel filtration
Pseudomonas stutzeri
Turnover Number [1/s]
1.20.1.1
7.33
phosphite
Pseudomonas stutzeri
pH Optimum
1.20.1.1
8
Pseudomonas stutzeri
pH Range
1.20.1.1
4.5
10
low activity
Pseudomonas stutzeri
Cloned(Commentary) (protein specific)
1.20.1.1
in Escherichia coli
Pseudomonas stutzeri
Inhibitors (protein specific)
1.20.1.1
arsenite
Pseudomonas stutzeri
1.20.1.1
D,L-hydroxyisocaproate
Pseudomonas stutzeri
1.20.1.1
D-2-hydroxy-4-methylvalerate
Pseudomonas stutzeri
1.20.1.1
D-glycerate
Pseudomonas stutzeri
1.20.1.1
formate
Pseudomonas stutzeri
1.20.1.1
NaCl
Pseudomonas stutzeri
1.20.1.1
NADH
competitive
Pseudomonas stutzeri
1.20.1.1
nitrite
Pseudomonas stutzeri
1.20.1.1
sulfite
dead end inhibitor, competitive
Pseudomonas stutzeri
KM Value [mM] (protein specific)
1.20.1.1
0.053
phosphite
Pseudomonas stutzeri
1.20.1.1
0.055
NAD+
Pseudomonas stutzeri
Molecular Weight [Da] (protein specific)
1.20.1.1
69000
gel filtration
Pseudomonas stutzeri
Natural Substrates/ Products (Substrates) (protein specific)
1.20.1.1
phosphite + H2O + NAD+
Pseudomonas stutzeri
use of phosphite as energy source, regeneration of NADH
phosphate + NADH + H+
Pseudomonas stutzeri
ir
Purification (Commentary) (protein specific)
1.20.1.1
recombinant protein
Pseudomonas stutzeri
Specific Activity [micromol/min/mg] (protein specific)
1.20.1.1
12.2
Pseudomonas stutzeri
Substrates and Products (Substrate) (protein specific)
1.20.1.1
hydroxypyruvate + NAD+
very poor substrate
287853
Pseudomonas stutzeri
pyruvate + NADH
287853
Pseudomonas stutzeri
?
1.20.1.1
phosphite + H2O + NAD+
287853
Pseudomonas stutzeri
phosphate + NADH + H+
287853
Pseudomonas stutzeri
ir
1.20.1.1
phosphite + H2O + NAD+
use of phosphite as energy source, regeneration of NADH
287853
Pseudomonas stutzeri
phosphate + NADH + H+
287853
Pseudomonas stutzeri
ir
1.20.1.1
phosphite + H2O + NADP+
NADP poorly substitutes for NAD+
287853
Pseudomonas stutzeri
phosphate + NADPH + H+
287853
Pseudomonas stutzeri
?
Subunits (protein specific)
1.20.1.1
homodimer
gel filtration
Pseudomonas stutzeri
Turnover Number [1/s] (protein specific)
1.20.1.1
7.33
phosphite
Pseudomonas stutzeri
pH Optimum (protein specific)
1.20.1.1
8
Pseudomonas stutzeri
pH Range (protein specific)
1.20.1.1
4.5
10
low activity
Pseudomonas stutzeri