show Purification and characterization of a novel phosphorus-oxidizing enzyme from Pseudomonas stutzeri WM88
Costas, A.M.G.; White, A.K.; Metcalf, W.W.; J. Biol. Chem. 276, 17429-17436 (2001)
Data extracted from this reference:
Cloned(Commentary)
EC Number
Commentary
Organism
1.20.1.1
in Escherichia coli
Pseudomonas stutzeri
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.20.1.1
arsenite
-
Pseudomonas stutzeri
1.20.1.1
D,L-hydroxyisocapronate
-
Pseudomonas stutzeri
1.20.1.1
D-2-hydroxy-4-methylvalerate
-
Pseudomonas stutzeri
1.20.1.1
D-glycerate
-
Pseudomonas stutzeri
1.20.1.1
formate
-
Pseudomonas stutzeri
1.20.1.1
NaCl
-
Pseudomonas stutzeri
1.20.1.1
NADH
competitive
Pseudomonas stutzeri
1.20.1.1
nitrite
-
Pseudomonas stutzeri
1.20.1.1
sulfite
dead end inhibitor, competitive
Pseudomonas stutzeri
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.20.1.1
0.053
-
phosphite
-
Pseudomonas stutzeri
1.20.1.1
0.055
-
NAD+
-
Pseudomonas stutzeri
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.20.1.1
69000
-
gel filtration
Pseudomonas stutzeri
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.20.1.1
phosphite + H2O + NAD+
Pseudomonas stutzeri
use of phosphite as energy source, regeneration of NADH
phosphate + NADH + H+
-
Pseudomonas stutzeri
ir
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.20.1.1
Pseudomonas stutzeri
O69054
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.20.1.1
recombinant protein
Pseudomonas stutzeri
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.20.1.1
12.2
-
-
Pseudomonas stutzeri
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.20.1.1
hydroxypyruvate + NAD+
very poor substrate
287853
Pseudomonas stutzeri
pyruvate + NADH
-
287853
Pseudomonas stutzeri
?
1.20.1.1
phosphite + H2O + NAD+
-
287853
Pseudomonas stutzeri
phosphate + NADH + H+
-
287853
Pseudomonas stutzeri
ir
1.20.1.1
phosphite + H2O + NAD+
use of phosphite as energy source, regeneration of NADH
287853
Pseudomonas stutzeri
phosphate + NADH + H+
-
287853
Pseudomonas stutzeri
ir
1.20.1.1
phosphite + H2O + NADP+
NADP poorly substitutes for NAD+
287853
Pseudomonas stutzeri
phosphate + NADPH + H+
-
287853
Pseudomonas stutzeri
?
Subunits
EC Number
Subunits
Commentary
Organism
1.20.1.1
homodimer
gel filtration
Pseudomonas stutzeri
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.20.1.1
7.33
-
phosphite
-
Pseudomonas stutzeri
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.20.1.1
8
-
-
Pseudomonas stutzeri
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.20.1.1
4.5
10
low activity
Pseudomonas stutzeri
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.20.1.1
in Escherichia coli
Pseudomonas stutzeri
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.20.1.1
arsenite
-
Pseudomonas stutzeri
1.20.1.1
D,L-hydroxyisocapronate
-
Pseudomonas stutzeri
1.20.1.1
D-2-hydroxy-4-methylvalerate
-
Pseudomonas stutzeri
1.20.1.1
D-glycerate
-
Pseudomonas stutzeri
1.20.1.1
formate
-
Pseudomonas stutzeri
1.20.1.1
NaCl
-
Pseudomonas stutzeri
1.20.1.1
NADH
competitive
Pseudomonas stutzeri
1.20.1.1
nitrite
-
Pseudomonas stutzeri
1.20.1.1
sulfite
dead end inhibitor, competitive
Pseudomonas stutzeri
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.20.1.1
0.053
-
phosphite
-
Pseudomonas stutzeri
1.20.1.1
0.055
-
NAD+
-
Pseudomonas stutzeri
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.20.1.1
69000
-
gel filtration
Pseudomonas stutzeri
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.20.1.1
phosphite + H2O + NAD+
Pseudomonas stutzeri
use of phosphite as energy source, regeneration of NADH
phosphate + NADH + H+
-
Pseudomonas stutzeri
ir
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.20.1.1
recombinant protein
Pseudomonas stutzeri
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.20.1.1
12.2
-
-
Pseudomonas stutzeri
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.20.1.1
hydroxypyruvate + NAD+
very poor substrate
287853
Pseudomonas stutzeri
pyruvate + NADH
-
287853
Pseudomonas stutzeri
?
1.20.1.1
phosphite + H2O + NAD+
-
287853
Pseudomonas stutzeri
phosphate + NADH + H+
-
287853
Pseudomonas stutzeri
ir
1.20.1.1
phosphite + H2O + NAD+
use of phosphite as energy source, regeneration of NADH
287853
Pseudomonas stutzeri
phosphate + NADH + H+
-
287853
Pseudomonas stutzeri
ir
1.20.1.1
phosphite + H2O + NADP+
NADP poorly substitutes for NAD+
287853
Pseudomonas stutzeri
phosphate + NADPH + H+
-
287853
Pseudomonas stutzeri
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.20.1.1
homodimer
gel filtration
Pseudomonas stutzeri
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.20.1.1
7.33
-
phosphite
-
Pseudomonas stutzeri
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.20.1.1
8
-
-
Pseudomonas stutzeri
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.20.1.1
4.5
10
low activity
Pseudomonas stutzeri