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Literature summary extracted from

  • Sundaram, T.K.; Snell, E.E.
    The bacterial oxidation of vitamin B6. V. The enzymatic formation of pyridoxal and isopyridoxal from pyridoxine (1969), J. Biol. Chem., 244, 2577-2584.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.12 1,10-phenanthroline
-
Pseudomonas sp.
1.1.3.12 3-chloromercuribenzoate o-chloromercuribenzoate, m-chloromercuribenzoate, and p-chloromercuribenzoate inhibit in absence but not in presence of cyanide Pseudomonas sp.
1.1.3.12 alpha,alpha'-dipyridyl
-
Pseudomonas sp.
1.1.3.12 o-chloromercuribenzoate o-chloromercuribenzoate, m-chloromercuribenzoate, and p-chloromercuribenzoate inhibit in absence but not in presence of cyanide Pseudomonas sp.
1.1.3.12 p-chloromercuribenzoate o-chloromercuribenzoate, m-chloromercuribenzoate, and p-chloromercuribenzoate inhibit in absence but not in presence of cyanide Pseudomonas sp.
1.1.99.9 additional information not inhibited by alpha,alpha'-dipyridyl, o-, m- or p-phenanthroline Pseudomonas sp.
1.1.99.9 p-chloromercuribenzoate
-
Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.12 0.0083
-
2,6-dichloroindophenol
-
Pseudomonas sp.
1.1.3.12 0.43
-
pyridoxine
-
Pseudomonas sp.
1.1.99.9 0.48
-
pyridoxine
-
Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.12 Pseudomonas sp.
-
MA-1
-
1.1.99.9 Pseudomonas sp.
-
IA
-
1.1.99.9 Pseudomonas sp. IA
-
IA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.12
-
Pseudomonas sp.
1.1.99.9 partial Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.12 additional information
-
-
Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.12 2-demethyl-2-ethylpyridoxine + O2
-
Pseudomonas sp. 2-demethyl-2-ethylpyridoxal + H2O2
-
?
1.1.3.12 5-deoxypyridoxine + O2
-
Pseudomonas sp. 5-deoxypyridoxal + H2O2
-
?
1.1.3.12 pyridoxine + 2,6-dichloroindophenol high degree of specificity for pyridoxine Pseudomonas sp. pyridoxol + reduced 2,6-dichlorophenolindophenol
-
?
1.1.3.12 pyridoxine + O2 other acceptor: 2,6-dichloroindophenol, high degree of specificity for pyridoxine Pseudomonas sp. pyridoxal + H2O2
-
?
1.1.3.12 pyridoxine + O2 other acceptor: 2,6-dichloroindophenol, high degree of specificity for pyridoxine Pseudomonas sp. IA pyridoxal + H2O2
-
?
1.1.99.9 pyridoxamine + acceptor low activity Pseudomonas sp. 4-aminomethyl-5-formyl-2-methylpyridin-3-ol + reduced acceptor
-
?
1.1.99.9 pyridoxamine + acceptor low activity Pseudomonas sp. IA 4-aminomethyl-5-formyl-2-methylpyridin-3-ol + reduced acceptor
-
?
1.1.99.9 pyridoxine + acceptor highly specific for pyridoxine Pseudomonas sp. isopyridoxal + reduced acceptor
-
?
1.1.99.9 pyridoxine + acceptor not: O2 Pseudomonas sp. isopyridoxal + reduced acceptor
-
?
1.1.99.9 pyridoxine + acceptor acceptor: 2,6-dichlorophenolindophenol Pseudomonas sp. isopyridoxal + reduced acceptor
-
?
1.1.99.9 pyridoxine + acceptor highly specific for pyridoxine Pseudomonas sp. IA isopyridoxal + reduced acceptor
-
?
1.1.99.9 pyridoxine + acceptor not: O2 Pseudomonas sp. IA isopyridoxal + reduced acceptor
-
?
1.1.99.9 pyridoxine + acceptor acceptor: 2,6-dichlorophenolindophenol Pseudomonas sp. IA isopyridoxal + reduced acceptor
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.12 26
-
assay at Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.12 7.5 8
-
Pseudomonas sp.
1.1.99.9 5.5
-
-
Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.12 additional information
-
-
Pseudomonas sp.
1.1.3.12 7 9 pH 7: 80% of maximal activity, pH 9: 50% of maximal acitvity Pseudomonas sp.
1.1.99.9 5 7 pH 5.0: about 30% of activity maximum, pH 7.0: 17% of activity maximum Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.12 FAD
-
Pseudomonas sp.
1.1.99.9 FAD flavoprotein, FAD-dependent Pseudomonas sp.