BRENDA - Enzyme Database

The (2R)-hydroxycarboxylate-viologen-oxidoreductase from Proteus vulgaris is a molybdenum-containing iron-sulphur protein

Trautwein, T.; Krauss, F.; Lottspeich, F.; Simon, H.; Eur. J. Biochem. 222, 1025-1032 (1994)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.99.6
KCN
5 mM, 50% inhibition after 7 min, complete inhibition after 1 h
Proteus vulgaris
1.1.99.30
CN-
-
Proteus vulgaris
1.1.99.30
O2
oxygen-sensitive
Proteus vulgaris
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.99.30
membrane
bound
Proteus vulgaris
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.99.6
Molybdenum
molybdenum containing enzyme
Proteus vulgaris
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.99.6
80000
-
x * 80000, probably octamer, SDS-PAGE
Proteus vulgaris
1.1.99.6
180000
600000
gel filtration, native PAGE
Proteus vulgaris
1.1.99.30
80000
-
8 * 80000, SDS-PAGE
Proteus vulgaris
1.1.99.30
600000
-
gel filtration
Proteus vulgaris
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.1.99.6
Proteus vulgaris
-
-
-
1.1.99.30
Proteus vulgaris
-
-
-
Oxidation Stability
EC Number
Oxidation Stability
Organism
1.1.99.6
oxygen-sensitive enzyme, has to be handled in the presence of detergent and absence of oxygen, if stirred under air at room temperatures 50% activity are lost within 30 min, the residual activity has a half-life of 10 h
Proteus vulgaris
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.1.99.6
DEAE-cellulose, hydroxyapatite, gel filtration
Proteus vulgaris
1.1.99.30
anaerobic conditions
Proteus vulgaris
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.99.30
additional information
anaerobic growth conditions
Proteus vulgaris
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.99.6
270
-
80000 Da form of the enzyme
Proteus vulgaris
1.1.99.6
1800
-
600000 Da form of the enzyme
Proteus vulgaris
1.1.99.30
1800
-
purified holoenzyme
Proteus vulgaris
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.1.99.6
(R)-lactate + acceptor
-
287841
Proteus vulgaris
pyruvate + reduced acceptor
-
287841
Proteus vulgaris
r
1.1.99.6
D-gluconate + acceptor
-
287841
Proteus vulgaris
2-oxo-gluconate + reduced acceptor
-
287841
Proteus vulgaris
r
1.1.99.6
D-glucose 6-phosphate + acceptor
-
287841
Proteus vulgaris
?
-
287841
Proteus vulgaris
r
1.1.99.6
N-acetylneuraminate + acceptor
-
287841
Proteus vulgaris
?
-
287841
Proteus vulgaris
r
1.1.99.6
phenylpyruvate + reduced acceptor
-
287841
Proteus vulgaris
phenyl-(R)-lactate + acceptor
-
287841
Proteus vulgaris
r
1.1.99.30
a (2R)-hydroxy-carboxylate + acceptor
-
287841
Proteus vulgaris
a 2-oxo-carboxylate + reduced acceptor
-
-
-
?
1.1.99.30
D-gluconate + oxidized 1,1'-carbamoyl methyl viologen
anaerobic conditions
287841
Proteus vulgaris
? + reduced 1,1'-carbamoyl methyl viologen
-
-
-
r
1.1.99.30
D-glucose 6-phosphate + oxidized 1,1'-carbamoyl methyl viologen
anaerobic conditions
287841
Proteus vulgaris
? + reduced 1,1'-carbamoyl methyl viologen
-
-
-
r
1.1.99.30
D-lactate + oxidized 1,1'-carbamoyl methyl viologen
anaerobic conditions
287841
Proteus vulgaris
pyruvate + reduced 1,1'-carbamoyl methyl viologen
-
-
-
r
1.1.99.30
N-acetylneuraminate + oxidized 1,1'-carbamoyl methyl viologen
anaerobic conditions
287841
Proteus vulgaris
? + reduced 1,1'-carbamoyl methyl viologen
-
-
-
r
1.1.99.30
phenylpyruvate + reduced 1,1'-carbamoyl methyl viologen
anaerobic conditions
287841
Proteus vulgaris
phenyllactate + oxidized 1,1'-carbamoyl methyl viologen
-
-
-
r
1.1.99.30
phenylpyruvate + reduced benzyl viologen
anaerobic conditions
287841
Proteus vulgaris
phenyllactate + oxidized benzyl viologen
-
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
1.1.99.6
oligomer
x * 80000, probably octamer, SDS-PAGE
Proteus vulgaris
1.1.99.30
More
subunit structure
Proteus vulgaris
1.1.99.30
octamer
8 * 80000, SDS-PAGE
Proteus vulgaris
Synonyms
EC Number
Synonyms
Commentary
Organism
1.1.99.30
HVOR
-
Proteus vulgaris
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.99.30
25
-
dehydrogenase assay at
Proteus vulgaris
1.1.99.30
37
-
reductase assay at
Proteus vulgaris
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.99.30
8.5
-
assay at
Proteus vulgaris
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.99.6
anthraquinone-2,6-disulfonate
artificial electron acceptor
Proteus vulgaris
1.1.99.6
molybdopterin mononucleotide
-
Proteus vulgaris
1.1.99.6
additional information
molybenum-containing Fe-S-enzyme without an additional prosthetic group such as flavin or cytochrome c, enzyme contains one molybdenum, four iron and four sulfur atoms per subunit
Proteus vulgaris
1.1.99.30
molybdenum cofactor
mononucleotide, 1 enzyme molecule contains 1 molybdenum atom
Proteus vulgaris
1.1.99.30
additional information
enzyme contains iron sulfur clusters, with four iron and 4 sulfur atoms per enzyme molecule, enzyme belongs to the group of molybdoenzymes which possess no further prosthetic group than the iron sulfur clusters
Proteus vulgaris
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.1.99.30
Proteus vulgaris
isoelectric focusing
-
4.9
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.99.6
anthraquinone-2,6-disulfonate
artificial electron acceptor
Proteus vulgaris
1.1.99.6
molybdopterin mononucleotide
-
Proteus vulgaris
1.1.99.6
additional information
molybenum-containing Fe-S-enzyme without an additional prosthetic group such as flavin or cytochrome c, enzyme contains one molybdenum, four iron and four sulfur atoms per subunit
Proteus vulgaris
1.1.99.30
molybdenum cofactor
mononucleotide, 1 enzyme molecule contains 1 molybdenum atom
Proteus vulgaris
1.1.99.30
additional information
enzyme contains iron sulfur clusters, with four iron and 4 sulfur atoms per enzyme molecule, enzyme belongs to the group of molybdoenzymes which possess no further prosthetic group than the iron sulfur clusters
Proteus vulgaris
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.99.6
KCN
5 mM, 50% inhibition after 7 min, complete inhibition after 1 h
Proteus vulgaris
1.1.99.30
CN-
-
Proteus vulgaris
1.1.99.30
O2
oxygen-sensitive
Proteus vulgaris
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.99.30
membrane
bound
Proteus vulgaris
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.99.6
Molybdenum
molybdenum containing enzyme
Proteus vulgaris
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.99.6
80000
-
x * 80000, probably octamer, SDS-PAGE
Proteus vulgaris
1.1.99.6
180000
600000
gel filtration, native PAGE
Proteus vulgaris
1.1.99.30
80000
-
8 * 80000, SDS-PAGE
Proteus vulgaris
1.1.99.30
600000
-
gel filtration
Proteus vulgaris
Oxidation Stability (protein specific)
EC Number
Oxidation Stability
Organism
1.1.99.6
oxygen-sensitive enzyme, has to be handled in the presence of detergent and absence of oxygen, if stirred under air at room temperatures 50% activity are lost within 30 min, the residual activity has a half-life of 10 h
Proteus vulgaris
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.99.6
DEAE-cellulose, hydroxyapatite, gel filtration
Proteus vulgaris
1.1.99.30
anaerobic conditions
Proteus vulgaris
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.99.30
additional information
anaerobic growth conditions
Proteus vulgaris
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.99.6
270
-
80000 Da form of the enzyme
Proteus vulgaris
1.1.99.6
1800
-
600000 Da form of the enzyme
Proteus vulgaris
1.1.99.30
1800
-
purified holoenzyme
Proteus vulgaris
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.1.99.6
(R)-lactate + acceptor
-
287841
Proteus vulgaris
pyruvate + reduced acceptor
-
287841
Proteus vulgaris
r
1.1.99.6
D-gluconate + acceptor
-
287841
Proteus vulgaris
2-oxo-gluconate + reduced acceptor
-
287841
Proteus vulgaris
r
1.1.99.6
D-glucose 6-phosphate + acceptor
-
287841
Proteus vulgaris
?
-
287841
Proteus vulgaris
r
1.1.99.6
N-acetylneuraminate + acceptor
-
287841
Proteus vulgaris
?
-
287841
Proteus vulgaris
r
1.1.99.6
phenylpyruvate + reduced acceptor
-
287841
Proteus vulgaris
phenyl-(R)-lactate + acceptor
-
287841
Proteus vulgaris
r
1.1.99.30
a (2R)-hydroxy-carboxylate + acceptor
-
287841
Proteus vulgaris
a 2-oxo-carboxylate + reduced acceptor
-
-
-
?
1.1.99.30
D-gluconate + oxidized 1,1'-carbamoyl methyl viologen
anaerobic conditions
287841
Proteus vulgaris
? + reduced 1,1'-carbamoyl methyl viologen
-
-
-
r
1.1.99.30
D-glucose 6-phosphate + oxidized 1,1'-carbamoyl methyl viologen
anaerobic conditions
287841
Proteus vulgaris
? + reduced 1,1'-carbamoyl methyl viologen
-
-
-
r
1.1.99.30
D-lactate + oxidized 1,1'-carbamoyl methyl viologen
anaerobic conditions
287841
Proteus vulgaris
pyruvate + reduced 1,1'-carbamoyl methyl viologen
-
-
-
r
1.1.99.30
N-acetylneuraminate + oxidized 1,1'-carbamoyl methyl viologen
anaerobic conditions
287841
Proteus vulgaris
? + reduced 1,1'-carbamoyl methyl viologen
-
-
-
r
1.1.99.30
phenylpyruvate + reduced 1,1'-carbamoyl methyl viologen
anaerobic conditions
287841
Proteus vulgaris
phenyllactate + oxidized 1,1'-carbamoyl methyl viologen
-
-
-
r
1.1.99.30
phenylpyruvate + reduced benzyl viologen
anaerobic conditions
287841
Proteus vulgaris
phenyllactate + oxidized benzyl viologen
-
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.99.6
oligomer
x * 80000, probably octamer, SDS-PAGE
Proteus vulgaris
1.1.99.30
More
subunit structure
Proteus vulgaris
1.1.99.30
octamer
8 * 80000, SDS-PAGE
Proteus vulgaris
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.99.30
25
-
dehydrogenase assay at
Proteus vulgaris
1.1.99.30
37
-
reductase assay at
Proteus vulgaris
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.99.30
8.5
-
assay at
Proteus vulgaris
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.1.99.30
Proteus vulgaris
isoelectric focusing
-
4.9