EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.99.6 | KCN | 5 mM, 50% inhibition after 7 min, complete inhibition after 1 h | Proteus vulgaris | |
1.1.99.30 | CN- | - |
Proteus vulgaris | |
1.1.99.30 | O2 | oxygen-sensitive | Proteus vulgaris |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.99.30 | membrane | bound | Proteus vulgaris | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.99.6 | Molybdenum | molybdenum containing enzyme | Proteus vulgaris |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.99.6 | 80000 | - |
x * 80000, probably octamer, SDS-PAGE | Proteus vulgaris |
1.1.99.6 | 180000 | 600000 | gel filtration, native PAGE | Proteus vulgaris |
1.1.99.30 | 80000 | - |
8 * 80000, SDS-PAGE | Proteus vulgaris |
1.1.99.30 | 600000 | - |
gel filtration | Proteus vulgaris |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.99.6 | Proteus vulgaris | - |
- |
- |
1.1.99.30 | Proteus vulgaris | - |
- |
- |
EC Number | Oxidation Stability | Organism |
---|---|---|
1.1.99.6 | oxygen-sensitive enzyme, has to be handled in the presence of detergent and absence of oxygen, if stirred under air at room temperatures 50% activity are lost within 30 min, the residual activity has a half-life of 10 h | Proteus vulgaris |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.99.6 | DEAE-cellulose, hydroxyapatite, gel filtration | Proteus vulgaris |
1.1.99.30 | anaerobic conditions | Proteus vulgaris |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.99.30 | additional information | anaerobic growth conditions | Proteus vulgaris | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.99.6 | 270 | - |
80000 Da form of the enzyme | Proteus vulgaris |
1.1.99.6 | 1800 | - |
600000 Da form of the enzyme | Proteus vulgaris |
1.1.99.30 | 1800 | - |
purified holoenzyme | Proteus vulgaris |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.99.6 | (R)-lactate + acceptor | - |
Proteus vulgaris | pyruvate + reduced acceptor | - |
r | |
1.1.99.6 | D-gluconate + acceptor | - |
Proteus vulgaris | 2-oxo-gluconate + reduced acceptor | - |
r | |
1.1.99.6 | D-glucose 6-phosphate + acceptor | - |
Proteus vulgaris | ? | - |
r | |
1.1.99.6 | N-acetylneuraminate + acceptor | - |
Proteus vulgaris | ? | - |
r | |
1.1.99.6 | phenylpyruvate + reduced acceptor | - |
Proteus vulgaris | phenyl-(R)-lactate + acceptor | - |
r | |
1.1.99.30 | a (2R)-hydroxy-carboxylate + acceptor | - |
Proteus vulgaris | a 2-oxo-carboxylate + reduced acceptor | - |
? | |
1.1.99.30 | D-gluconate + oxidized 1,1'-carbamoyl methyl viologen | anaerobic conditions | Proteus vulgaris | ? + reduced 1,1'-carbamoyl methyl viologen | - |
r | |
1.1.99.30 | D-glucose 6-phosphate + oxidized 1,1'-carbamoyl methyl viologen | anaerobic conditions | Proteus vulgaris | ? + reduced 1,1'-carbamoyl methyl viologen | - |
r | |
1.1.99.30 | D-lactate + oxidized 1,1'-carbamoyl methyl viologen | anaerobic conditions | Proteus vulgaris | pyruvate + reduced 1,1'-carbamoyl methyl viologen | - |
r | |
1.1.99.30 | N-acetylneuraminate + oxidized 1,1'-carbamoyl methyl viologen | anaerobic conditions | Proteus vulgaris | ? + reduced 1,1'-carbamoyl methyl viologen | - |
r | |
1.1.99.30 | phenylpyruvate + reduced 1,1'-carbamoyl methyl viologen | anaerobic conditions | Proteus vulgaris | phenyllactate + oxidized 1,1'-carbamoyl methyl viologen | - |
r | |
1.1.99.30 | phenylpyruvate + reduced benzyl viologen | anaerobic conditions | Proteus vulgaris | phenyllactate + oxidized benzyl viologen | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.99.6 | oligomer | x * 80000, probably octamer, SDS-PAGE | Proteus vulgaris |
1.1.99.30 | More | subunit structure | Proteus vulgaris |
1.1.99.30 | octamer | 8 * 80000, SDS-PAGE | Proteus vulgaris |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.99.30 | HVOR | - |
Proteus vulgaris |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.99.30 | 25 | - |
dehydrogenase assay at | Proteus vulgaris |
1.1.99.30 | 37 | - |
reductase assay at | Proteus vulgaris |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.99.30 | 8.5 | - |
assay at | Proteus vulgaris |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.99.6 | anthraquinone-2,6-disulfonate | artificial electron acceptor | Proteus vulgaris | |
1.1.99.6 | molybdopterin mononucleotide | - |
Proteus vulgaris | |
1.1.99.6 | additional information | molybenum-containing Fe-S-enzyme without an additional prosthetic group such as flavin or cytochrome c, enzyme contains one molybdenum, four iron and four sulfur atoms per subunit | Proteus vulgaris | |
1.1.99.30 | molybdenum cofactor | mononucleotide, 1 enzyme molecule contains 1 molybdenum atom | Proteus vulgaris | |
1.1.99.30 | additional information | enzyme contains iron sulfur clusters, with four iron and 4 sulfur atoms per enzyme molecule, enzyme belongs to the group of molybdoenzymes which possess no further prosthetic group than the iron sulfur clusters | Proteus vulgaris |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.1.99.30 | Proteus vulgaris | isoelectric focusing | - |
4.9 |