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Literature summary extracted from

  • Kaufman, E.E.; Nelson, T.; Fales, H.M.; Levin, D.M.
    Isolation and characterization of a hydroxyacid-oxoacid transhydrogenase from rat kidney mitochondria (1988), J. Biol. Chem., 263, 16872-16879.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.99.24 NAD+ stabilizes during ammonium sulfate precipitation Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.99.24 additional information not inhibited by anaerobiosis Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.24 0.0046
-
Succinic semialdehyde
-
Rattus norvegicus
1.1.99.24 0.018
-
2-oxoglutarate
-
Rattus norvegicus
1.1.99.24 0.3
-
4-hydroxybutyrate
-
Rattus norvegicus
1.1.99.24 0.42
-
D-2-hydroxyglutarate
-
Rattus norvegicus
1.1.99.24 3
-
L-3-hydroxybutyrate
-
Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.99.24 mitochondrion
-
Rattus norvegicus 5739
-

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.24 Rattus norvegicus
-
Sprague-Dawley strain
-
1.1.99.24 Rattus norvegicus Sprague-Dawley
-
Sprague-Dawley strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.24 partial Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.99.24 brain
-
Rattus norvegicus
-
1.1.99.24 kidney
-
Rattus norvegicus
-
1.1.99.24 liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.99.24 0.0513
-
-
Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.24 4-hydroxybutyrate + 2-oxoglutarate hydrogen attached to hydroxyl-bearing C of 4-hydroxybutyrate is transferred to ketone-bearing C of 2-oxoglutarate, oxoacid not NAD+ or NADP+ acts as hydrogen acceptor Rattus norvegicus succinic semialdehyde + 2-hydroxyglutarate
-
r
1.1.99.24 4-hydroxybutyrate + 2-oxoglutarate hydrogen attached to hydroxyl-bearing C of 4-hydroxybutyrate is transferred to ketone-bearing C of 2-oxoglutarate, oxoacid not NAD+ or NADP+ acts as hydrogen acceptor Rattus norvegicus Sprague-Dawley succinic semialdehyde + 2-hydroxyglutarate
-
r
1.1.99.24 D-2-hydroxyglutarate + succinic semialdehyde
-
Rattus norvegicus 2-oxoglutarate + 4-hydroxybutyrate
-
?
1.1.99.24 D-2-hydroxyglutarate + succinic semialdehyde
-
Rattus norvegicus Sprague-Dawley 2-oxoglutarate + 4-hydroxybutyrate
-
?
1.1.99.24 L-3-hydroxybutyrate + 2-oxoglutarate oxidized at 33% the rate of 4-hydroxybutyrate Rattus norvegicus acetoacetate + 2-oxoglutarate
-
?
1.1.99.24 L-3-hydroxybutyrate + 2-oxoglutarate oxidized at 33% the rate of 4-hydroxybutyrate Rattus norvegicus Sprague-Dawley acetoacetate + 2-oxoglutarate
-
?
1.1.99.24 L-3-hydroxybutyrate + succinic semialdehyde oxo-acids that can act as hydrogen acceptors are 2-oxoadipate, 24% as effective as 2-oxoglutarate and oxaloacetate, 19% as effective as 2-oxoglutarate, D-3-hydroxybutyrate, DL-2-hydroxybutyrate, D/L-malate, D/L-lactate, acetoacetate, 3-oxobutyrate, 3-oxoadipate do not act as hydrogen acceptors, poor acceptors are pyruvate and 2-oxobutyrate Rattus norvegicus acetoacetate + 4-hydroxybutyrate
-
?
1.1.99.24 L-3-hydroxybutyrate + succinic semialdehyde 62.5% of activity with D-2-hydroxyglutarate Rattus norvegicus acetoacetate + 4-hydroxybutyrate
-
?
1.1.99.24 L-3-hydroxybutyrate + succinic semialdehyde oxo-acids that can act as hydrogen acceptors are 2-oxoadipate, 24% as effective as 2-oxoglutarate and oxaloacetate, 19% as effective as 2-oxoglutarate, D-3-hydroxybutyrate, DL-2-hydroxybutyrate, D/L-malate, D/L-lactate, acetoacetate, 3-oxobutyrate, 3-oxoadipate do not act as hydrogen acceptors, poor acceptors are pyruvate and 2-oxobutyrate Rattus norvegicus Sprague-Dawley acetoacetate + 4-hydroxybutyrate
-
?
1.1.99.24 L-3-hydroxybutyrate + succinic semialdehyde 62.5% of activity with D-2-hydroxyglutarate Rattus norvegicus Sprague-Dawley acetoacetate + 4-hydroxybutyrate
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.99.24 37
-
assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.99.24 7
-
-
Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.24 additional information reaction does not depend on NAD+ or NADP+ Rattus norvegicus