EC Number | General Stability | Organism |
---|---|---|
1.1.99.24 | NAD+ stabilizes during ammonium sulfate precipitation | Rattus norvegicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.99.24 | additional information | not inhibited by anaerobiosis | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.99.24 | 0.0046 | - |
Succinic semialdehyde | - |
Rattus norvegicus | |
1.1.99.24 | 0.018 | - |
2-oxoglutarate | - |
Rattus norvegicus | |
1.1.99.24 | 0.3 | - |
4-hydroxybutyrate | - |
Rattus norvegicus | |
1.1.99.24 | 0.42 | - |
D-2-hydroxyglutarate | - |
Rattus norvegicus | |
1.1.99.24 | 3 | - |
L-3-hydroxybutyrate | - |
Rattus norvegicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.99.24 | mitochondrion | - |
Rattus norvegicus | 5739 | - |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.99.24 | Rattus norvegicus | - |
Sprague-Dawley strain | - |
1.1.99.24 | Rattus norvegicus Sprague-Dawley | - |
Sprague-Dawley strain | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.99.24 | partial | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.99.24 | brain | - |
Rattus norvegicus | - |
1.1.99.24 | kidney | - |
Rattus norvegicus | - |
1.1.99.24 | liver | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.99.24 | 0.0513 | - |
- |
Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.99.24 | 4-hydroxybutyrate + 2-oxoglutarate | hydrogen attached to hydroxyl-bearing C of 4-hydroxybutyrate is transferred to ketone-bearing C of 2-oxoglutarate, oxoacid not NAD+ or NADP+ acts as hydrogen acceptor | Rattus norvegicus | succinic semialdehyde + 2-hydroxyglutarate | - |
r | |
1.1.99.24 | 4-hydroxybutyrate + 2-oxoglutarate | hydrogen attached to hydroxyl-bearing C of 4-hydroxybutyrate is transferred to ketone-bearing C of 2-oxoglutarate, oxoacid not NAD+ or NADP+ acts as hydrogen acceptor | Rattus norvegicus Sprague-Dawley | succinic semialdehyde + 2-hydroxyglutarate | - |
r | |
1.1.99.24 | D-2-hydroxyglutarate + succinic semialdehyde | - |
Rattus norvegicus | 2-oxoglutarate + 4-hydroxybutyrate | - |
? | |
1.1.99.24 | D-2-hydroxyglutarate + succinic semialdehyde | - |
Rattus norvegicus Sprague-Dawley | 2-oxoglutarate + 4-hydroxybutyrate | - |
? | |
1.1.99.24 | L-3-hydroxybutyrate + 2-oxoglutarate | oxidized at 33% the rate of 4-hydroxybutyrate | Rattus norvegicus | acetoacetate + 2-oxoglutarate | - |
? | |
1.1.99.24 | L-3-hydroxybutyrate + 2-oxoglutarate | oxidized at 33% the rate of 4-hydroxybutyrate | Rattus norvegicus Sprague-Dawley | acetoacetate + 2-oxoglutarate | - |
? | |
1.1.99.24 | L-3-hydroxybutyrate + succinic semialdehyde | oxo-acids that can act as hydrogen acceptors are 2-oxoadipate, 24% as effective as 2-oxoglutarate and oxaloacetate, 19% as effective as 2-oxoglutarate, D-3-hydroxybutyrate, DL-2-hydroxybutyrate, D/L-malate, D/L-lactate, acetoacetate, 3-oxobutyrate, 3-oxoadipate do not act as hydrogen acceptors, poor acceptors are pyruvate and 2-oxobutyrate | Rattus norvegicus | acetoacetate + 4-hydroxybutyrate | - |
? | |
1.1.99.24 | L-3-hydroxybutyrate + succinic semialdehyde | 62.5% of activity with D-2-hydroxyglutarate | Rattus norvegicus | acetoacetate + 4-hydroxybutyrate | - |
? | |
1.1.99.24 | L-3-hydroxybutyrate + succinic semialdehyde | oxo-acids that can act as hydrogen acceptors are 2-oxoadipate, 24% as effective as 2-oxoglutarate and oxaloacetate, 19% as effective as 2-oxoglutarate, D-3-hydroxybutyrate, DL-2-hydroxybutyrate, D/L-malate, D/L-lactate, acetoacetate, 3-oxobutyrate, 3-oxoadipate do not act as hydrogen acceptors, poor acceptors are pyruvate and 2-oxobutyrate | Rattus norvegicus Sprague-Dawley | acetoacetate + 4-hydroxybutyrate | - |
? | |
1.1.99.24 | L-3-hydroxybutyrate + succinic semialdehyde | 62.5% of activity with D-2-hydroxyglutarate | Rattus norvegicus Sprague-Dawley | acetoacetate + 4-hydroxybutyrate | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.99.24 | 37 | - |
assay at | Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.99.24 | 7 | - |
- |
Rattus norvegicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.99.24 | additional information | reaction does not depend on NAD+ or NADP+ | Rattus norvegicus |