Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ameyama, M.; Shinagawa, E.; Matsushita, K.; Adachi, O.
    Solubilization, purification and properties of membrane-bound glycerol dehydrogenase from Gluconobacter industrius (1985), Agric. Biol. Chem., 49, 1001-1010.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.1.99.22 amphoteric detergents, such as dimethyldodecylamineoxide, lauroylsarcosine, dodecylsulfobetaine, lauroylbetaine are effective solubilizers Gluconobacter oxydans
1.1.99.22 dimethyldodecylamineoxide, 0.5%, stabilizes Gluconobacter oxydans
1.1.99.22 glycerol stabilizes Gluconobacter oxydans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.99.22 EDTA
-
 Gluconobacter oxydans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.22 2.3
-
 glycerol membrane fraction Gluconobacter oxydans
1.1.99.22 19
-
 meso-erythritol
-
 Gluconobacter oxydans
1.1.99.22 22
-
 D-arabitol
-
 Gluconobacter oxydans
1.1.99.22 34
-
 glycerol solubilized enzyme Gluconobacter oxydans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.99.22 membrane
-
 Gluconobacter oxydans 16020
-

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.22 Gluconobacter oxydans
-
 maximum activity at the end of exponential growth phase, activity occurs in various Gluconobacter species, such as Gluconobacter cerinus, Gluconobacter gluconicus, Gluconobacter suboxydans and Gluconobacter albidus
-
1.1.99.22 Gluconobacter oxydans IFO 3260
-
 maximum activity at the end of exponential growth phase, activity occurs in various Gluconobacter species, such as Gluconobacter cerinus, Gluconobacter gluconicus, Gluconobacter suboxydans and Gluconobacter albidus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.22
-
 Gluconobacter oxydans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.99.22 52
-
-
 Gluconobacter oxydans

Storage Stability

EC Number Storage Stability Organism
1.1.99.22 -20°C, solubilized, stable for 6 months Gluconobacter oxydans
1.1.99.22 2-4°C, diluted enzyme solution, stable for 2 weeks Gluconobacter oxydans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.22 adonitol + acceptor 42% of activity with glycerol Gluconobacter oxydans ?
-
 ?
1.1.99.22 adonitol + acceptor 42% of activity with glycerol Gluconobacter oxydans IFO 3260 ?
-
 ?
1.1.99.22 D-arabitol + acceptor 136% of activity with glycerol oxidation Gluconobacter oxydans ?
-
 ?
1.1.99.22 D-arabitol + acceptor 136% of activity with glycerol oxidation Gluconobacter oxydans IFO 3260 ?
-
 ?
1.1.99.22 D-mannitol + acceptor 26% of activity with glycerol Gluconobacter oxydans D-fructose + reduced acceptor
-
 ?
1.1.99.22 D-sorbitol + acceptor 45% of acticvity with glycerol Gluconobacter oxydans L-sorbose + reduced acceptor
-
 ?
1.1.99.22 D-sorbitol + acceptor 45% of acticvity with glycerol Gluconobacter oxydans IFO 3260 L-sorbose + reduced acceptor
-
 ?
1.1.99.22 galactitol + acceptor i.e. dulcitol, 29% of activity with glycerol Gluconobacter oxydans ?
-
 ?
1.1.99.22 glycerol + acceptor 2,6-dichlorophenolindophenol/phenazine methosulfate act as in vitro acceptor, broad specificity, no substrates are: xylitol, D-fructose, D-glucose, D-gluconate, 2-keto-D-gluconate, ethanol and acetaldehyde Gluconobacter oxydans glycerone + reduced acceptor i.e. dihydroxyacetone ?
1.1.99.22 glycerol + acceptor 2,6-dichlorophenolindophenol/phenazine methosulfate act as in vitro acceptor, broad specificity, no substrates are: xylitol, D-fructose, D-glucose, D-gluconate, 2-keto-D-gluconate, ethanol and acetaldehyde Gluconobacter oxydans IFO 3260 glycerone + reduced acceptor i.e. dihydroxyacetone ?
1.1.99.22 meso-erythritol + acceptor 111% of activity with glycerol Gluconobacter oxydans ?
-
 ?
1.1.99.22 meso-erythritol + acceptor 111% of activity with glycerol Gluconobacter oxydans IFO 3260 ?
-
 ?
1.1.99.22 propylene glycol + acceptor 54% of activity with glycerol Gluconobacter oxydans ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.99.22 More could be identical with non-purified EC 1.1.2.2 or D-arabitol dehydrogenase Gluconobacter oxydans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.99.22 25
-
-
 Gluconobacter oxydans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.99.22 7.5 8
-
 Gluconobacter oxydans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.99.22 8.5 10 stable in the presence of glycerol and detergent Gluconobacter oxydans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.22 2,6-dichlorophenolindophenol acts in vitro as acceptor together with phenazine methosulfate Gluconobacter oxydans
1.1.99.22 additional information NAD+, NADP+ or molecular oxygen do not act as acceptors Gluconobacter oxydans
1.1.99.22 phenazine methosulfate acts in vitro as acceptor together with 2,6-dichlorophenolindophenol, linked to electron-transport chain of the organism Gluconobacter oxydans
1.1.99.22 pyrroloquinoline quinone quinoprotein Gluconobacter oxydans