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Literature summary extracted from

  • Baminger, U.; Subramaniam, S.S.; Renganathan, V.; Halterich, d.
    Purification and characterization of cellobiose dehydrogenase from the plant pathogen Sclerotium (Athelia) rolfsii (2001), Appl. Environ. Microbiol., 67, 1766-1774.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.99.18 lactobiono-1,5-lactone weak product inhibition Athelia rolfsii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.18 0.0003
-
cellobiose electron acceptor cytochrome c from horse heart Athelia rolfsii
1.1.99.18 0.0004
-
cellobiose electron acceptor 2,2'-azino-di-(3-ethyl-benzthiazolin-6-sulfonic acid) cation radical Athelia rolfsii
1.1.99.18 0.002
-
cellobiose electron acceptor K3Fe(CN)6 Athelia rolfsii
1.1.99.18 0.0044
-
cellobiose electron acceptors methylen green and blue Athelia rolfsii
1.1.99.18 0.015
-
cellobiose electron acceptor 2,6-dichloroindophenol Athelia rolfsii
1.1.99.18 0.025
-
cellobiose electron acceptor 1,4-benzoquinone Athelia rolfsii
1.1.99.18 0.031
-
cellobiose electron acceptor meldola blue Athelia rolfsii
1.1.99.18 0.053
-
cellobiose electron acceptor 3,5-di-tert-butyl-1,2-benzoquinone Athelia rolfsii
1.1.99.18 0.12
-
cellobiose
-
Athelia rolfsii
1.1.99.18 0.49
-
cellotriose
-
Athelia rolfsii
1.1.99.18 0.54
-
cellopentaose
-
Athelia rolfsii
1.1.99.18 0.55
-
cellobiose electron acceptor 2,6-dimethyl-1,4-benzoquinone Athelia rolfsii
1.1.99.18 0.6
-
cellotetraose
-
Athelia rolfsii
1.1.99.18 2
-
beta-lactose 70-75% Athelia rolfsii
1.1.99.18 2.3
-
thiocellobiose
-
Athelia rolfsii
1.1.99.18 2.4
-
lactose
-
Athelia rolfsii
1.1.99.18 5.4
-
xylobiose
-
Athelia rolfsii
1.1.99.18 54
-
alpha-lactose
-
Athelia rolfsii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.99.18 extracellular
-
Athelia rolfsii
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.99.18 101000
-
MALDI-MS Athelia rolfsii
1.1.99.18 110000
-
1 * 110000, SDS-PAGE Athelia rolfsii
1.1.99.18 126000
-
native PAGE Athelia rolfsii

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.18 Athelia rolfsii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.18 DEAE sepharose, Resource PHE Athelia rolfsii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.99.18 culture medium
-
Athelia rolfsii
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.99.18 63
-
electron acceptor 2,6-dichloroindophenol Athelia rolfsii
1.1.99.18 66
-
electron acceptor cytochrome c Athelia rolfsii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.18 alpha-lactose + acceptor
-
Athelia rolfsii 4-O-(alpha-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
-
?
1.1.99.18 beta-lactose + acceptor
-
Athelia rolfsii 4-O-(beta-D-glucopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
-
?
1.1.99.18 cellobiose + acceptor artificial electron acceptors: 2,6-dichlorophenolindophenol, 1,4-benzoquinone, 2,6-dimethyl-1,4-benzoquinone, 2,6-dimethoxy-1,4-benzoquinone, 3,5-di-tert-butyl-1,2-benzoquinone, methylene blue and green, meldola blue, 2,2'-azino-di-(3-ethyl-benzthiazolin-6-sulfonic acid), cytochrome c, K3Fe(CN)6 Athelia rolfsii cellobiono-1,5-lactone + reduced acceptor
-
?
1.1.99.18 cellopentaose + acceptor
-
Athelia rolfsii cellopentaono-1,5-lactone + reduced acceptor
-
?
1.1.99.18 cellotetraose + acceptor
-
Athelia rolfsii cellotetrono-1,5-lactone + reduced acceptor
-
?
1.1.99.18 cellotriose + acceptor
-
Athelia rolfsii cellotriono-1,5-lactone + reduced acceptor
-
?
1.1.99.18 lactose + acceptor
-
Athelia rolfsii 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
-
?
1.1.99.18 thiocellobiose + acceptor
-
Athelia rolfsii thiocellobiono-1,5-lactone + reduced acceptor
-
?
1.1.99.18 xylobiose + 2,6-dichlorophenolindophenol
-
Athelia rolfsii 4-O-beta-D-xylopyranosyl-D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
r

Subunits

EC Number Subunits Comment Organism
1.1.99.18 monomer 1 * 110000, SDS-PAGE Athelia rolfsii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.99.18 5
-
cellobiose electron acceptor 2,6-dimethoxy-1,4-benzoquinone Athelia rolfsii
1.1.99.18 15
-
cellobiose electron acceptor 2,6-dimethyl-1,4-benzoquinone Athelia rolfsii
1.1.99.18 17
-
alpha-lactose
-
Athelia rolfsii
1.1.99.18 18
-
cellobiose electron acceptors methylene blue or methylene green Athelia rolfsii
1.1.99.18 23
-
cellobiose electron acceptor 3,5-di-tert-butyl-1,2-benzoquinone Athelia rolfsii
1.1.99.18 24
-
cellotetraose
-
Athelia rolfsii
1.1.99.18 24
-
cellopentaose
-
Athelia rolfsii
1.1.99.18 24
-
cellobiose electron acceptor meldola blue Athelia rolfsii
1.1.99.18 26
-
lactose
-
Athelia rolfsii
1.1.99.18 26
-
cellotriose
-
Athelia rolfsii
1.1.99.18 27
-
cellobiose
-
Athelia rolfsii
1.1.99.18 27
-
xylobiose
-
Athelia rolfsii
1.1.99.18 27
-
thiocellobiose
-
Athelia rolfsii
1.1.99.18 27
-
cellobiose electron acceptor 2,2'-azino-di-(3-ethyl-benzthiazolin-6-sulfonic acid) cation radical Athelia rolfsii
1.1.99.18 30
-
cellobiose 2,6-dichloroindophenpl or 1,4-benzoquinone as electron acceptor Athelia rolfsii
1.1.99.18 34
-
cellobiose electron acceptor cytochrome c from horseheart Athelia rolfsii
1.1.99.18 37
-
cellobiose electron acceptor K3Fe(CN)6 Athelia rolfsii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.99.18 2.7 5 depends on electron acceptor Athelia rolfsii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.99.18 5.7
-
at 35°C, enzyme loses 50% of its initial activity after 10 min at pH 2.2, stability optimum shifts to 5.0 at 46°C with a half-life of 12 h Athelia rolfsii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.18 FAD 1 heme per enzyme molecule, 1 FAD nucleotide per molecule Athelia rolfsii
1.1.99.18 heme b 1 heme per enzyme molecule, 1 FAD nucleotide per molecule Athelia rolfsii
1.1.99.18 additional information limited proteolytic cleavage of the enzyme leads to an inactive heme peptide and an active FAD domain which has been termed cellobiose: quinone 1-oxidoreductase EC 1.5.1.1 Athelia rolfsii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.99.18 31
-
lactobiono-1,5-lactone
-
Athelia rolfsii