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Literature summary extracted from
- Characterization of a cellobiose dehydrogenase from Humicola insolens (1998), Biochem. J., 330, 565-571.
No PubMed abstract available
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.99.18 | 0.011 | - |
cellobiose | - |
Humicola insolens |  |
| 1.1.99.18 | 0.012 | - |
ferricyanide | - |
Humicola insolens | |
| 1.1.99.18 | 0.017 | - |
cellopentaose | - |
Humicola insolens | |
| 1.1.99.18 | 0.019 | - |
cellotriose | - |
Humicola insolens | |
| 1.1.99.18 | 0.021 | - |
cellotetraose | - |
Humicola insolens | |
| 1.1.99.18 | 0.026 | - |
2,6-dichlorphenolindophenol | - |
Humicola insolens | |
| 1.1.99.18 | 0.051 | - |
lactose | - |
Humicola insolens | |
| 1.1.99.18 | 0.093 | - |
cytochrome c | - |
Humicola insolens | |
| 1.1.99.18 | 0.132 | - |
benzoquinone | - |
Humicola insolens | |
| 1.1.99.18 | 7.1 | - |
xylobiose | - |
Humicola insolens | |
| 1.1.99.18 | 11 | - |
maltose | - |
Humicola insolens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.1.99.18 | extracellular | - |
Humicola insolens | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.18 | 92000 | - |
gel filtration | Humicola insolens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.99.18 | Humicola insolens | - |
soft-rot thermophilic fungus | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.99.18 | arginine sepharose, S-sepharose, HPQ-sepharose, Superdex | Humicola insolens |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.18 | 4409 | - |
- |
Humicola insolens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.99.18 | cellobiose + 2,6-dichlorophenolindophenol | - |
Humicola insolens | cellobiono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
r | |
| 1.1.99.18 | cellobiose + acceptor | electron acceptors: 2,6-dichlorophenolindophenol, benzoquinone, ferricyanide, cytochrome c, methylene blue, O2 | Humicola insolens | cellobiono-1,5-lactone + reduced acceptor | - |
? | |
| 1.1.99.18 | cellopentaose + acceptor | - |
Humicola insolens | cellopentaono-1,5-lactone + reduced acceptor | - |
? | |
| 1.1.99.18 | cellotetraose + acceptor | - |
Humicola insolens | cellotetrono-1,5-lactone + reduced acceptor | - |
? | |
| 1.1.99.18 | cellotriose + acceptor | - |
Humicola insolens | cellotriono-1,5-lactone + reduced acceptor | - |
? | |
| 1.1.99.18 | lactose + acceptor | - |
Humicola insolens | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor | - |
? | |
| 1.1.99.18 | maltose + acceptor | - |
Humicola insolens | 4-O-(alpha-D-glucopyranosyl)-D-glucono-1,5-lactone + reduced acceptor | - |
? | |
| 1.1.99.18 | xylobiose + acceptor | - |
Humicola insolens | 4-O-beta-D-xylopyranosyl-D-xylono-1,5-lactone + reduced acceptor | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.99.18 | monomer | 1 * 92000, SDS-PAGE | Humicola insolens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.18 | 65 | - |
sharp drop above | Humicola insolens |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.99.18 | 0.118 | - |
xylobiose | - |
Humicola insolens | |
| 1.1.99.18 | 1.2 | - |
maltose | - |
Humicola insolens | |
| 1.1.99.18 | 3 | - |
xylobiose | - |
Humicola insolens | |
| 1.1.99.18 | 12 | - |
cellotriose | - |
Humicola insolens | |
| 1.1.99.18 | 12 | - |
cellotetraose | - |
Humicola insolens | |
| 1.1.99.18 | 12 | - |
cellopentaose | - |
Humicola insolens | |
| 1.1.99.18 | 14 | - |
cellobiose | - |
Humicola insolens | |
| 1.1.99.18 | 14 | - |
lactose | - |
Humicola insolens | |
| 1.1.99.18 | 14 | - |
ferricyanide | - |
Humicola insolens | |
| 1.1.99.18 | 17 | - |
2,6-dichlorphenolindophenol | - |
Humicola insolens | |
| 1.1.99.18 | 21 | - |
benzoquinone | - |
Humicola insolens | |
| 1.1.99.18 | 27 | - |
cytochrome c | - |
Humicola insolens | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.18 | 4 | - |
methylen blue as electron acceptor | Humicola insolens |
| 1.1.99.18 | 7 | - |
2,6-dichlorophenolindophenol as electron acceptor | Humicola insolens |
| 1.1.99.18 | 7 | - |
3,5-di-tert-butyl-1,2-benzoquinone as electron acceptor | Humicola insolens |
| 1.1.99.18 | 7 | 9 | cytochrome c as electron acceptor | Humicola insolens |
| 1.1.99.18 | 9 | - |
ferricyanide as electron acceptor | Humicola insolens |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.99.18 | FAD | - |
Humicola insolens | |
| 1.1.99.18 | heme b | - |
Humicola insolens | |
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Release 2023.1 (January 2023)
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