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Literature summary extracted from

  • Schmidhalter, D.R.; Canevascini, G.
    Isolation and characterization of the cellobiose dehydrogenase from brown-rot fungus Coniophora puteana (Schum ex Fr.)Karst. (1993), Arch. Biochem. Biophys., 300, 559-563.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.99.18 cellobiose strong substrate inhibition Coniophora puteana (Schum ex Fr) Karsten

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.99.18 extracellular
-
 Coniophora puteana (Schum ex Fr) Karsten
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.99.18 111000
-
 2 * 111000, SDS-PAGE Coniophora puteana (Schum ex Fr) Karsten
1.1.99.18 192000
-
 molecular weight of the enzyme is reduced by 11000 Da upon treatment with endo H, gel filtration Coniophora puteana (Schum ex Fr) Karsten

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.18 Coniophora puteana (Schum ex Fr) Karsten
-
 brown rot fungus
-
1.1.99.18 Coniophora puteana (Schum ex Fr) Karsten EMPA 62
-
 brown rot fungus
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.99.18 glycoprotein 13% sugar as mannose Coniophora puteana (Schum ex Fr) Karsten

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.18 Q Sepharose, DEAE TSK 650 S, Superose 12 Coniophora puteana (Schum ex Fr) Karsten

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.99.18 culture medium
-
 Coniophora puteana (Schum ex Fr) Karsten
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.99.18 2.18
-
-
 Coniophora puteana (Schum ex Fr) Karsten

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.18 cellobiose + acceptor 2,6-dichlorophenolindophenol as electron acceptor Coniophora puteana (Schum ex Fr) Karsten cellobiono-1,5-lactone + reduced acceptor
-
 ?
1.1.99.18 cellobiose + acceptor 2,6-dichlorophenolindophenol as electron acceptor Coniophora puteana (Schum ex Fr) Karsten EMPA 62 cellobiono-1,5-lactone + reduced acceptor
-
 ?
1.1.99.18 cellohexaose + acceptor
-
 Coniophora puteana (Schum ex Fr) Karsten cellohexaono-1,5-lactone + reduced acceptor
-
 ?
1.1.99.18 cellopentaose + acceptor
-
 Coniophora puteana (Schum ex Fr) Karsten cellopentaono-1,5-lactone + reduced acceptor
-
 ?
1.1.99.18 cellopentaose + acceptor
-
 Coniophora puteana (Schum ex Fr) Karsten EMPA 62 cellopentaono-1,5-lactone + reduced acceptor
-
 ?
1.1.99.18 cellotetraose + acceptor
-
 Coniophora puteana (Schum ex Fr) Karsten cellotetrono-1,5-lactone + reduced acceptor
-
 ?
1.1.99.18 cellotetraose + acceptor
-
 Coniophora puteana (Schum ex Fr) Karsten EMPA 62 cellotetrono-1,5-lactone + reduced acceptor
-
 ?
1.1.99.18 cellotriose + acceptor
-
 Coniophora puteana (Schum ex Fr) Karsten cellotriono-1,5-lactone + reduced acceptor
-
 ?
1.1.99.18 cellotriose + acceptor
-
 Coniophora puteana (Schum ex Fr) Karsten EMPA 62 cellotriono-1,5-lactone + reduced acceptor
-
 ?
1.1.99.18 lactose + acceptor
-
 Coniophora puteana (Schum ex Fr) Karsten 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
-
 ?
1.1.99.18 lactose + acceptor
-
 Coniophora puteana (Schum ex Fr) Karsten EMPA 62 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.99.18 dimer 2 * 111000, SDS-PAGE Coniophora puteana (Schum ex Fr) Karsten

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.18 FAD
-
 Coniophora puteana (Schum ex Fr) Karsten
1.1.99.18 heme b
-
 Coniophora puteana (Schum ex Fr) Karsten

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.99.18 5.4
-
 cellobiose
-
 Coniophora puteana (Schum ex Fr) Karsten