EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.99.12 | CoCl2 | strain DSM 4025, 5 mM, 84% inhibition | Gluconobacter oxydans | |
1.1.99.12 | EDTA | strain DSM 4025, 5 mM, 87% inhibition | Gluconobacter oxydans | |
1.1.99.12 | EGTA | strain DSM 4025, 5 mM, 89% inhibition | Gluconobacter oxydans | |
1.1.99.12 | FeCl2 | strain DSM 4025, 5 mM, 89% inhibition | Gluconobacter oxydans | |
1.1.99.12 | FeCl3 | strain DSM 4025, 5 mM, 97% inhibition | Gluconobacter oxydans | |
1.1.99.12 | NiSO4 | strain DSM 4025, 5 mM, 77% inhibition | Gluconobacter oxydans | |
1.1.99.12 | ZnCl2 | strain DSM 4025, 5 mM, 90% inhibition | Gluconobacter oxydans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.99.12 | 2 | - |
1-propanol | strain DSM 4025 | Gluconobacter oxydans | |
1.1.99.12 | 230 | - |
L-sorbose | strain DSM 4025 | Gluconobacter oxydans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.99.12 | 62500 | - |
strain DSM 4025, 1 * 62500 + 1 * 64500, SDS-PAGE | Gluconobacter oxydans |
1.1.99.12 | 64500 | - |
strain DSM 4025, 1 * 62500 + 1 * 64500, SDS-PAGE | Gluconobacter oxydans |
1.1.99.12 | 135000 | - |
strain DSM 4025, gel filtration | Gluconobacter oxydans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.99.12 | L-sorbose + acceptor | Gluconobacter oxydans | strain DSM 4025, enzyme has both L-sorbose dehydrogenase and L-sorbosone dehydrogenase activity, 10.9% of activity with 1-propanol | L-sorbosone + reduced acceptor | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.99.12 | Gluconobacter oxydans | - |
strain DSM 4025 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.99.12 | strain DSM 4025, PEG 600, DEAE-Toyopearl, Q-Sepharose | Gluconobacter oxydans |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.99.12 | 6.73 | - |
strain DSM 4025 | Gluconobacter oxydans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.99.12 | 1-butanol + acceptor | strain DSM 4025, 69.4% of activity with 1-propanol | Gluconobacter oxydans | n-butanal + reduced acceptor | - |
? | |
1.1.99.12 | 1-pentanol + acceptor | strain DSM 4025, 49.8% of activity with 1-propanol | Gluconobacter oxydans | n-pentanal + reduced acceptor | - |
? | |
1.1.99.12 | 1-propanol + acceptor | - |
Gluconobacter oxydans | n-propanal + reduced acceptor | - |
? | |
1.1.99.12 | 2-butanol + acceptor | strain DSM 4025, 87.4% of activity with 1-propanol | Gluconobacter oxydans | 2-butanone + reduced acceptor | - |
? | |
1.1.99.12 | 2-propanol + acceptor | strain DSM 4025, 78.2% of activity with 1-propanol | Gluconobacter oxydans | acetone + reduced acceptor | - |
? | |
1.1.99.12 | D-fructose + acceptor | strain DSM 4025, 6.6% of activity with 1-propanol | Gluconobacter oxydans | 2-keto-D-gulonate + reduced acceptor | - |
? | |
1.1.99.12 | D-glucose + acceptor | strain DSM 4025, 25.3% of activity with 1-propanol | Gluconobacter oxydans | D-gluconic acid + reduced acceptor | - |
? | |
1.1.99.12 | D-mannitol + acceptor | strain DSM 4025, 16.2% of activity with 1-propanol | Gluconobacter oxydans | ? + reduced acceptor | - |
? | |
1.1.99.12 | D-sorbitol + acceptor | strain DSM 4025, 7.1% of activity with 1-propanol | Gluconobacter oxydans | D-glucose + D-gluconic acid + L-sorbose + reduced acceptor | - |
? | |
1.1.99.12 | DL-glyceraldehyde + acceptor | strain DSM 4025, 80.2% of activity with 1-propanol | Gluconobacter oxydans | ? + reduced acceptor | - |
? | |
1.1.99.12 | ethanol + acceptor | strain DSM 4025, 88.6% of activity with 1-propanol | Gluconobacter oxydans | ethanal + reduced acceptor | - |
? | |
1.1.99.12 | L-sorbose + acceptor | strain DSM 4025, enzyme has both L-sorbose dehydrogenase and L-sorbosone dehydrogenase activity, 10.9% of activity with 1-propanol | Gluconobacter oxydans | L-sorbosone + reduced acceptor | - |
? | |
1.1.99.12 | L-sorbosone + acceptor | strain DSM 4025, 16.2% of activity with 1-propanol | Gluconobacter oxydans | 2-keto-L-gulonate + reduced acceptor | - |
? | |
1.1.99.12 | n-heptanol + acceptor | strain DSM 4025, 47.7% of activity with 1-propanol | Gluconobacter oxydans | n-heptanal + reduced acceptor | - |
? | |
1.1.99.12 | n-hexanol + acceptor | strain DSM 4025, 49.1% of activity with 1-propanol | Gluconobacter oxydans | n-hexanal + reduced acceptor | - |
? | |
1.1.99.12 | polyethylene glycol 100 + acceptor | strain DSM 4025, 68.9% of activity with 1-propanol | Gluconobacter oxydans | ? | - |
? | |
1.1.99.12 | polyvinyl alcohol + acceptor | strain DSM 4025, 38.1% of activity with 1-propanol | Gluconobacter oxydans | oxidized polyvinyl alcohol + reduced acceptor | - |
? | |
1.1.99.12 | propionaldehyde + acceptor | strain DSM 4025, 83.3% of activity with 1-propanol | Gluconobacter oxydans | propanoate + reduced acceptor | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.99.12 | dimer | strain DSM 4025, 1 * 62500 + 1 * 64500, SDS-PAGE | Gluconobacter oxydans |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.99.12 | 30 | 40 | strain DSM 4025 | Gluconobacter oxydans |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.99.12 | 10 | 50 | strain DSM 4025 | Gluconobacter oxydans |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.99.12 | additional information | - |
strain DSM 4025, enzyme is stable at and below 30°C, but unstable above 40°C | Gluconobacter oxydans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.99.12 | 7 | 9 | strain DSM 4025 | Gluconobacter oxydans |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.1.99.12 | additional information | - |
strain DSM 4025, enzyme is stable in alkaline pH and unstable with increasing acidity | Gluconobacter oxydans |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.99.12 | cytochrome c | strain DSM 4025, physiological electron acceptor | Gluconobacter oxydans | |
1.1.99.12 | pyrroloquinoline quinone | strain DSM 4025, non-covalently bound, 2 mol PQQ/mol enzyme | Gluconobacter oxydans |