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Literature summary extracted from
- Regulation of succinate dehydrogenase and tautomerization of oxaloacetate (1989), Adv. Enzyme Regul., 28, 271-280.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.2.2 | Acid-labile sulfur | enzyme form OAT-2 contains 2 atoms of acid-labile sulfur | Bos taurus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.3.5.1 | malonate | - |
Bos taurus |  |
| 1.3.5.1 | N-ethylmaleimide | - |
Bos taurus | |
| 1.3.5.1 | oxaloacetate | - |
Bos taurus | |
| 5.3.2.2 | diphosphate | enzyme form OAT-2 is inhibited, enzyme form OAT-1 not | Bos taurus | |
| 5.3.2.2 | Fluorocitrate | inhibition of OAT-2, tautomerase reaction and aconitase reaction | Bos taurus | |
| 5.3.2.2 | Maleate | enzyme form OAT-2 is inhibited, enzyme form OAT-1 not | Bos taurus | |
| 5.3.2.2 | NEM | enzyme form OAT-2 is inhibited, enzyme form OAT-1 not | Bos taurus | |
| 5.3.2.2 | oxalate | enzyme form OAT-1 is inhibited, enzyme form OAT-2 not | Bos taurus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.3.5.1 | 0.1 | - |
succinate | - |
Bos taurus | |
| 1.3.5.1 | 1.5 | - |
D-malate | - |
Bos taurus | |
| 1.3.5.1 | 2.2 | - |
L-malate | - |
Bos taurus | |
| 5.3.2.2 | 0.045 | - |
enol-oxaloacetate | enzyme form OAT-1 | Bos taurus | |
| 5.3.2.2 | 0.068 | - |
keto-oxaloacetate | enzyme form OAT-1 | Bos taurus | |
| 5.3.2.2 | 220 | - |
enol-oxaloacetate | enzyme form OAT-2 | Bos taurus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.3.5.1 | mitochondrion | - |
Bos taurus | 5739 | - |
| 5.3.2.2 | mitochondrial matrix | - |
Bos taurus | 5759 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.2.2 | Fe | enzyme form OAT-2 contains 2 atoms of non-heme Fe per mol | Bos taurus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.3.2.2 | 37000 | - |
enzyme form OAT-1 | Bos taurus |
| 5.3.2.2 | 80000 | - |
enzyme form OAT-2 | Bos taurus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.3.5.1 | additional information | Bos taurus | succinate dehydrogenase is a component of the respiratory chain and operates as a compulsory member of the Krebs cycle in mammals | ? | - |
? | |
| 1.3.5.1 | succinate + ubiquinone | Bos taurus | - |
fumarate + ubiquinol | - |
? | |
| 5.3.2.2 | additional information | Bos taurus | significant role of enzymatic oxaloacetate tautomerization in the control of the succinate dehydrogenase activity in the mitochondrial matrix | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.3.5.1 | Bos taurus | - |
succinate dehydrogenase | - |
| 5.3.2.2 | Bos taurus | - |
enzyme forms OAT-1 and OAT-2 | - |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.3.5.1 | heart | - |
Bos taurus | - |
| 5.3.2.2 | heart | - |
Bos taurus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.3.5.1 | D-malate + N,N,N',N'-tetramethyl 4-phenylenediamine | - |
Bos taurus | oxaloacetate + reduced N,N,N',N'-tetramethyl 4-phenylenediamine | - |
r | |
| 1.3.5.1 | additional information | L- or D-malate oxidation | Bos taurus | ? | - |
? | |
| 1.3.5.1 | additional information | succinate dehydrogenase is a component of the respiratory chain and operates as a compulsory member of the Krebs cycle in mammals | Bos taurus | ? | - |
? | |
| 1.3.5.1 | succinate + ubiquinone | - |
Bos taurus | fumarate + ubiquinol | - |
? | |
| 1.3.5.1 | ubiquinone-1 + L-malate | - |
Bos taurus | ? | - |
? | |
| 5.3.2.2 | keto-Oxaloacetate | r | Bos taurus | Enol-oxaloacetate | - |
? | |
| 5.3.2.2 | additional information | OAT-2 catalyzes aconitase reaction after treatment with Fe2+ | Bos taurus | ? | - |
? | |
| 5.3.2.2 | additional information | significant role of enzymatic oxaloacetate tautomerization in the control of the succinate dehydrogenase activity in the mitochondrial matrix | Bos taurus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.3.2.2 | monomer | 1 * 37000, enzyme form OAT-1 | Bos taurus |
| 5.3.2.2 | monomer | 1 * 80000, enzyme form OAT-2 | Bos taurus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.2.2 | 40 | - |
enzyme form OAT-1: no inactivation | Bos taurus |
| 5.3.2.2 | 40 | - |
enzyme form OAT-2: t1/2: about 15 min | Bos taurus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.3.5.1 | 0.142 | - |
L-malate | - |
Bos taurus | |
| 1.3.5.1 | 0.283 | - |
D-malate | - |
Bos taurus | |
| 1.3.5.1 | 28.3 | - |
succinate | - |
Bos taurus | |
| 5.3.2.2 | 3.58 | - |
keto-oxaloacetate | enzyme form OAT-1, 25°C, pH 9.0 | Bos taurus | |
| 5.3.2.2 | 26.7 | - |
enol-oxaloacetate | enzyme form OAT-2, 25°C, pH 9.0 | Bos taurus | |
| 5.3.2.2 | 45 | - |
enol-oxaloacetate | enzyme form OAT-1, 25°C, pH 9.0 | Bos taurus | |
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