EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.1.3.40 | additional information | no requirement or activation by sulfhydryl reagents, such as DTT | Cornu aspersum |
EC Number | General Stability | Organism |
---|---|---|
1.1.3.40 | addition of EDTA and/or DTT does not appreciably improve stability | Cornu aspersum |
1.1.3.40 | glycerol stabilizes | Cornu aspersum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.3.40 | deoxycholate | 1% w/v: 36% inhibition, deoxycholate, 1%, readily releases membrane-bound activity, but is slightly inhibitory | Cornu aspersum | |
1.1.3.40 | additional information | no inhibition by cyanide and metal chelators such as EDTA | Cornu aspersum | |
1.1.3.40 | n-butanol | n-butyl alcohol | Cornu aspersum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.3.40 | 0.04 | - |
O2 | oxygen, substrate: D-mannitol | Cornu aspersum | |
1.1.3.40 | 5.9 | - |
D-mannitol | - |
Cornu aspersum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.3.40 | Golgi membrane | membrane-bound | Cornu aspersum | 139 | - |
1.1.3.40 | mitochondrion | - |
Cornu aspersum | 5739 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.3.40 | additional information | - |
mannitol oxidase activity elutes near the void volume of Sephacryl S300 columns which suggests a high molecular weight | Cornu aspersum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.3.40 | D-mannitol + O2 | Cornu aspersum | because of high mannitol content of many plants, the herbivorous diet of terrestrial snails, and the association of mannitol oxidase with their digestive tract, the snail enzyme may represent a unique nutritional adaption of these and possibly other herbivorous molluscs | mannose + H2O2 | - |
? | |
1.1.3.40 | additional information | Cornu aspersum | because of high mannitol content of many plants, the herbivorous diet of terrestrial snails, and the association of mannitol oxidase with their digestive tract, the snail enzyme may represent a unique nutritional adaption of these and possibly other herbivorous molluscs | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.3.40 | Cornu aspersum | - |
common garden snail | - |
1.1.3.40 | Cornu aspersum | - |
terrestrial snail | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
1.1.3.40 | no modification | not a glycoprotein, the presence of amino sugar residues cannot be ruled out | Cornu aspersum |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.3.40 | solubilization and partial purification. Both ionic and non-ionic as well as zwitterionic detergents are effective in solubilizing mannitol oxidase, deoxycholate, 1%, readily releases membrane-bound activity, but is slightly inhibitory | Cornu aspersum |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.1.3.40 | D-mannitol + O2 = D-mannose + H2O2 | utilization of the sugar alcohols generally proceeds by an initial oxidation, producing a hexose | Cornu aspersum | |
1.1.3.40 | D-mannitol + O2 = D-mannose + H2O2 | stoichiometry, mannose/H2O2 = 0.86 | Cornu aspersum |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.3.40 | alimentary canal | - |
Cornu aspersum | - |
1.1.3.40 | crop | highest activity | Cornu aspersum | - |
1.1.3.40 | digestive gland | hepatopancreas | Cornu aspersum | - |
1.1.3.40 | gut | inner lining of lumen of the gut | Cornu aspersum | - |
1.1.3.40 | additional information | occurs in several tissues in terrestrial snails, concentrated within the alimentary tract and digestive glands with highest activity in the crop | Cornu aspersum | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.3.40 | 0.984 | - |
- |
Cornu aspersum |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.3.40 | -20°C, 20% glycerol, one week, remains active | Cornu aspersum |
1.1.3.40 | 5°C, Tris buffer, 10% glycerol, 4 days, 35% activity retained | Cornu aspersum |
1.1.3.40 | 5°C, Tris buffer, 20% glycerol, 4 days, 69% activity retained | Cornu aspersum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.3.40 | D,L-threitol + O2 | 7% activity compared with D-arabinitol | Cornu aspersum | ? + H2O2 | - |
? | |
1.1.3.40 | D-arabinitol + O2 | best substrate | Cornu aspersum | arabinose + H2O2 | - |
? | |
1.1.3.40 | D-glucitol + O2 | sorbitol | Cornu aspersum | D-glucose + H2O2 | - |
? | |
1.1.3.40 | D-glucitol + O2 | 20% activity compared with D-arabinitol | Cornu aspersum | D-glucose + H2O2 | - |
? | |
1.1.3.40 | D-glycero-D-galactoheptitol + O2 | 9% activity compared with D-arabinitol | Cornu aspersum | ? + H2O2 | - |
? | |
1.1.3.40 | D-glycero-D-galactoheptitol + O2 | perseitol | Cornu aspersum | ? + H2O2 | - |
? | |
1.1.3.40 | D-mannitol + O2 | 93% activity compared with D-arabinitol | Cornu aspersum | mannose + H2O2 | - |
? | |
1.1.3.40 | D-mannitol + O2 | because of high mannitol content of many plants, the herbivorous diet of terrestrial snails, and the association of mannitol oxidase with their digestive tract, the snail enzyme may represent a unique nutritional adaption of these and possibly other herbivorous molluscs | Cornu aspersum | mannose + H2O2 | - |
? | |
1.1.3.40 | galactitol + O2 | 16% activity compared with D-arabinitol | Cornu aspersum | D-galactose + H2O2 | - |
? | |
1.1.3.40 | galactitol + O2 | dulcitol | Cornu aspersum | D-galactose + H2O2 | - |
? | |
1.1.3.40 | additional information | substrate specificity | Cornu aspersum | ? | - |
? | |
1.1.3.40 | additional information | configuration around carbon-2 and carbon-4 is critical for binding and reactivity, reactivity requires trans-configuration of the oxygens at carbons 2 and 4 | Cornu aspersum | ? | - |
? | |
1.1.3.40 | additional information | utilization of the sugar alcohols generally proceeds by an initial oxidation, producing a hexose | Cornu aspersum | ? | - |
? | |
1.1.3.40 | additional information | activity only with acyclic polyols | Cornu aspersum | ? | - |
? | |
1.1.3.40 | additional information | no or very poor activity with L-arabinitol, glycerol, ribitol, erythritol, inositol, aplha-glycerol-phosphate, xylitol, ascorbate, D-glucose and D-galactose | Cornu aspersum | ? | - |
? | |
1.1.3.40 | additional information | because of high mannitol content of many plants, the herbivorous diet of terrestrial snails, and the association of mannitol oxidase with their digestive tract, the snail enzyme may represent a unique nutritional adaption of these and possibly other herbivorous molluscs | Cornu aspersum | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.3.40 | More | - |
Cornu aspersum |
1.1.3.40 | oligomer | two major peptides, one, possibly a doublet, of 68000 Da and the other of higher mass. Two faint, faster moving peptides located between 45000 and 68000 Da, SDS-PAGE | Cornu aspersum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.3.40 | 25 | - |
assay at | Cornu aspersum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.3.40 | additional information | - |
alkaline pH-optimum | Cornu aspersum |
1.1.3.40 | additional information | - |
pI: 5.4-5.6 | Cornu aspersum |
1.1.3.40 | 8 | 8.5 | alkaline pH-optimum | Cornu aspersum |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.3.40 | 6.5 | 10 | at pH 6.5 and pH 10.0: about 50% of maximum activity | Cornu aspersum |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.1.3.40 | additional information | - |
enzyme relatively stable at alkaline pH | Cornu aspersum |
1.1.3.40 | 11 | - |
enzyme relatively stable at alkaline pH, pH 11: 30% of maximum activity remains | Cornu aspersum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.3.40 | additional information | no evidence for a flavin or heme cofactor | Cornu aspersum | |
1.1.3.40 | additional information | pyridine nucleotide or cytochrome c not required | Cornu aspersum |