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Literature summary extracted from

  • Shimao, M.; Ninomiyaaa, K.; Kuno, O.; Kato, N.; Sakazawa, C.
    Existence of a novel enzyme, pyrroloquinoline quinone-dependent polyvinyl alcohol dehydrogenase, in a bacterial symbiont, Pseudomonas sp. strain VM15C (1986), Appl. Environ. Microbiol., 51, 268-275.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.2.6 membrane
-
 Pseudomonas sp. 16020
-
1.1.3.30 extracellular amount depending on growth rate Pseudomonas sp.
-
-
1.1.3.30 periplasm
-
 Pseudomonas sp.
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.30 31000
-
 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.30 polyvinyl alcohol + O2 Pseudomonas sp.
-
 oxidized polyvinyl alcohol + H2O2
-
 ?
1.1.3.30 polyvinyl alcohol + O2 Pseudomonas sp. VM15C
-
 oxidized polyvinyl alcohol + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.2.6 Pseudomonas sp.
-
-
-
1.1.2.6 Pseudomonas sp. VM15C
-
-
-
1.1.3.30 Pseudomonas sp.
-
 in symbiotic culture with Pseudomonas putida during PVA utilization
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.2.6 partial Pseudomonas sp.
1.1.3.30
-
 Pseudomonas sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.30 culture supernatant
-
 Pseudomonas sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.30 1.68
-
 polyvinyl alcohol Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.2.6 additional information the enzyme shows dehydrogenase activity with 2,5-hexanediol, 4-heptanol, 2-hexanol, and 2-pentanol at 20-45% of the activity with polyvinyl alcohol Pseudomonas sp. ?
-
 ?
1.1.2.6 polyvinyl alcohol + acceptor pyrroloquinoline quinone-dependent polyvinyl alcohol dehydrogenase requires pyrroloquinoline quinone for polyvinyl alcohol dehydrogenation with phenazine methosulfate, phenazine ethosulfate, and 2,6-dichlorophenolindophenol as electron acceptors Pseudomonas sp. polyvinyl alcohol containing oxo-groups + reduced acceptor
-
 ?
1.1.3.30 2,5-hexanediol + O2 4.9% of activity with polyvinyl alcohol Pseudomonas sp. 2,5-hexanedione + H2O2
-
 ?
1.1.3.30 2-hexanol + O2 101% of activity with polyvinyl alcohol Pseudomonas sp. 2-hexanone + H2O2
-
 ?
1.1.3.30 2-hexanol + O2 101% of activity with polyvinyl alcohol Pseudomonas sp. VM15C 2-hexanone + H2O2
-
 ?
1.1.3.30 2-pentanol + O2 58% of activity with polyvinyl alcohol Pseudomonas sp. 2-pentanone + H2O2
-
 ?
1.1.3.30 2-pentanol + O2 58% of activity with polyvinyl alcohol Pseudomonas sp. VM15C 2-pentanone + H2O2
-
 ?
1.1.3.30 2-propanol + O2 4.9% of activity with polyvinyl alcohol Pseudomonas sp. acetone + H2O2
-
 ?
1.1.3.30 2-propanol + O2 4.9% of activity with polyvinyl alcohol Pseudomonas sp. VM15C acetone + H2O2
-
 ?
1.1.3.30 4-heptanol + O2 91% of activity with polyvinyl alcohol Pseudomonas sp. 4-heptanone + H2O2
-
 ?
1.1.3.30 4-heptanol + O2 91% of activity with polyvinyl alcohol Pseudomonas sp. VM15C 4-heptanone + H2O2
-
 ?
1.1.3.30 polyvinyl alcohol + O2
-
 Pseudomonas sp. oxidized polyvinyl alcohol + H2O2
-
 ?
1.1.3.30 polyvinyl alcohol + O2
-
 Pseudomonas sp. VM15C oxidized polyvinyl alcohol + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.2.6 PVA dehydrogenase
-
 Pseudomonas sp.
1.1.2.6 pyrroloquinoline quinone-dependent polyvinyl alcohol dehydrogenase
-
 Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.2.6 30
-
 assay at Pseudomonas sp.
1.1.3.30 30
-
 assay at Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.2.6 7.5
-
 assay at Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.2.6 pyrroloquinoline quinone
-
 Pseudomonas sp.
1.1.3.30 additional information no requirement for pyrroloquinoline quinone (PQQ) as does the PQQ-dependent PVA dehydrogenase Pseudomonas sp.