BRENDA - Enzyme Database show

Properties of a new enzyme, nucleoside oxidase, from Pseudomonas maltophilia LB-86

Isono, Y.; Sudo, T.; Hoshino, M.; Agric. Biol. Chem. 53, 1671-1677 (1989)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.3.28
HgCl2
30.8% inhibition at 1 mM
Stenotrophomonas maltophilia
1.1.3.28
KCN
65.2% inhibition at 1 mM, complete inhibition at 10 mM
Stenotrophomonas maltophilia
1.1.3.28
N-bromosuccinimide
complete inhibition at 1 mM
Stenotrophomonas maltophilia
1.1.3.28
NaN3
18.5% inhibition at 1 mM
Stenotrophomonas maltophilia
1.1.3.28
Pb(CH3COO)2
18.7% inhibition at 1 mM
Stenotrophomonas maltophilia
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.3.28
0.044
-
Inosine
-
Stenotrophomonas maltophilia
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.3.28
Fe2+
2 g non-heme iron per mol of enzyme, contains also a heme component
Stenotrophomonas maltophilia
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.3.28
14000
-
alpha,beta,gamma,delta 1 * 76000 + 1 * 33000 + 1 * 18000 + 1 * 14000, SDS-PAGE, subunits beta and delta appear to be linked together by disulfide bonds
Stenotrophomonas maltophilia
1.1.3.28
18000
-
alpha,beta,gamma,delta 1 * 76000 + 1 * 33000 + 1 * 18000 + 1 * 14000, SDS-PAGE, subunits beta and delta appear to be linked together by disulfide bonds
Stenotrophomonas maltophilia
1.1.3.28
33000
-
alpha,beta,gamma,delta 1 * 76000 + 1 * 33000 + 1 * 18000 + 1 * 14000, SDS-PAGE, subunits beta and delta appear to be linked together by disulfide bonds
Stenotrophomonas maltophilia
1.1.3.28
76000
-
alpha,beta,gamma,delta 1 * 76000 + 1 * 33000 + 1 * 18000 + 1 * 14000, SDS-PAGE, subunits beta and delta appear to be linked together by disulfide bonds
Stenotrophomonas maltophilia
1.1.3.28
130000
-
gel filtration
Stenotrophomonas maltophilia
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.3.28
inosine + O2
Stenotrophomonas maltophilia
oxidation of various nucleosides, but no oxidation of nucleotides, bases and ribose
9-riburonosylhypoxanthine + H2O
-
Stenotrophomonas maltophilia
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.3.28
Stenotrophomonas maltophilia
-
-
-
1.1.3.28
Stenotrophomonas maltophilia LB-86
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.3.28
to homogeneity, chromatography techniques
Stenotrophomonas maltophilia
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.3.28
cell culture
-
Stenotrophomonas maltophilia
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.3.28
2'-deoxyadenosine + O2
81.4% of the activity compared to inosine
287612
Stenotrophomonas maltophilia
9-(2-deoxy-beta-D-erythro-pentofuranuronosyl)-9H-purin-6-amine + H2O
-
287612
Stenotrophomonas maltophilia
?
1.1.3.28
adenosine + O2
97.3% of the activity compared to inosine
287612
Stenotrophomonas maltophilia
9-riburonosyladenine + H2O
-
287612
Stenotrophomonas maltophilia
?
1.1.3.28
adenosine + O2
97.3% of the activity compared to inosine
287612
Stenotrophomonas maltophilia LB-86
9-riburonosyladenine + H2O
-
287612
Stenotrophomonas maltophilia LB-86
?
1.1.3.28
cytidine + O2
77.3% of the activity compared to inosine
287612
Stenotrophomonas maltophilia
1-riburonosylcytosine + H2O
-
287612
Stenotrophomonas maltophilia
?
1.1.3.28
cytidine + O2
77.3% of the activity compared to inosine
287612
Stenotrophomonas maltophilia LB-86
1-riburonosylcytosine + H2O
-
287612
Stenotrophomonas maltophilia LB-86
?
1.1.3.28
deoxycytidine + O2
77.3% of the activity compared to inosine
287612
Stenotrophomonas maltophilia
1-riburonosylcytosine + H2O
-
287612
Stenotrophomonas maltophilia
?
1.1.3.28
deoxyguanosine + O2
92.3% of the activity compared to inosine
287612
Stenotrophomonas maltophilia
9-riburonosyldeoxyguanine + H2O
-
287612
Stenotrophomonas maltophilia
?
1.1.3.28
deoxyinosine + O2
80.7% of the activity compared to inosine
287612
Stenotrophomonas maltophilia
9-riburonosyldeoxyhypoxanthine + H2O
-
287612
Stenotrophomonas maltophilia
?
1.1.3.28
guanosine + O2
121% of the activity compared to inosine
287612
Stenotrophomonas maltophilia
9-riburonosylguanine + H2O
-
287612
Stenotrophomonas maltophilia
?
1.1.3.28
guanosine + O2
121% of the activity compared to inosine
287612
Stenotrophomonas maltophilia LB-86
9-riburonosylguanine + H2O
-
287612
Stenotrophomonas maltophilia LB-86
?
1.1.3.28
inosine + O2
oxidation of various nucleosides, but no oxidation of nucleotides, bases and ribose
287612
Stenotrophomonas maltophilia
9-riburonosylhypoxanthine + H2O
-
287612
Stenotrophomonas maltophilia
?
1.1.3.28
thymidine + O2
55.8% of the activity compared to inosine
287612
Stenotrophomonas maltophilia
1-riburonosylthymine + H2O
-
287612
Stenotrophomonas maltophilia
?
1.1.3.28
uridine + O2
-
287612
Stenotrophomonas maltophilia
1-riburonosyluracil + H2O
-
287612
Stenotrophomonas maltophilia
?
1.1.3.28
uridine + O2
-
287612
Stenotrophomonas maltophilia LB-86
1-riburonosyluracil + H2O
-
287612
Stenotrophomonas maltophilia LB-86
?
1.1.3.28
xanthosine + O2
best substrate tested, 125% of the activity compared to inosine
287612
Stenotrophomonas maltophilia
9-riburonosylxanthine + H2O
-
287612
Stenotrophomonas maltophilia
?
1.1.3.28
xanthosine + O2
best substrate tested, 125% of the activity compared to inosine
287612
Stenotrophomonas maltophilia LB-86
9-riburonosylxanthine + H2O
-
287612
Stenotrophomonas maltophilia LB-86
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.3.28
heterotetramer
alpha,beta,gamma,delta 1 * 76000 + 1 * 33000 + 1 * 18000 + 1 * 14000, SDS-PAGE, subunits beta and delta appear to be linked together by disulfide bonds
Stenotrophomonas maltophilia
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.3.28
60
-
stable below, complete loss of activity at 75°C
Stenotrophomonas maltophilia
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.3.28
5
6
-
Stenotrophomonas maltophilia
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.1.3.28
5
6
stable for 1 h at 37°C
Stenotrophomonas maltophilia
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.3.28
FAD
1 FAD covalently bound to the alpha subunit
Stenotrophomonas maltophilia
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.3.28
FAD
1 FAD covalently bound to the alpha subunit
Stenotrophomonas maltophilia
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.3.28
HgCl2
30.8% inhibition at 1 mM
Stenotrophomonas maltophilia
1.1.3.28
KCN
65.2% inhibition at 1 mM, complete inhibition at 10 mM
Stenotrophomonas maltophilia
1.1.3.28
N-bromosuccinimide
complete inhibition at 1 mM
Stenotrophomonas maltophilia
1.1.3.28
NaN3
18.5% inhibition at 1 mM
Stenotrophomonas maltophilia
1.1.3.28
Pb(CH3COO)2
18.7% inhibition at 1 mM
Stenotrophomonas maltophilia
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.3.28
0.044
-
Inosine
-
Stenotrophomonas maltophilia
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.3.28
Fe2+
2 g non-heme iron per mol of enzyme, contains also a heme component
Stenotrophomonas maltophilia
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.3.28
14000
-
alpha,beta,gamma,delta 1 * 76000 + 1 * 33000 + 1 * 18000 + 1 * 14000, SDS-PAGE, subunits beta and delta appear to be linked together by disulfide bonds
Stenotrophomonas maltophilia
1.1.3.28
18000
-
alpha,beta,gamma,delta 1 * 76000 + 1 * 33000 + 1 * 18000 + 1 * 14000, SDS-PAGE, subunits beta and delta appear to be linked together by disulfide bonds
Stenotrophomonas maltophilia
1.1.3.28
33000
-
alpha,beta,gamma,delta 1 * 76000 + 1 * 33000 + 1 * 18000 + 1 * 14000, SDS-PAGE, subunits beta and delta appear to be linked together by disulfide bonds
Stenotrophomonas maltophilia
1.1.3.28
76000
-
alpha,beta,gamma,delta 1 * 76000 + 1 * 33000 + 1 * 18000 + 1 * 14000, SDS-PAGE, subunits beta and delta appear to be linked together by disulfide bonds
Stenotrophomonas maltophilia
1.1.3.28
130000
-
gel filtration
Stenotrophomonas maltophilia
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.3.28
inosine + O2
Stenotrophomonas maltophilia
oxidation of various nucleosides, but no oxidation of nucleotides, bases and ribose
9-riburonosylhypoxanthine + H2O
-
Stenotrophomonas maltophilia
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.3.28
to homogeneity, chromatography techniques
Stenotrophomonas maltophilia
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.3.28
cell culture
-
Stenotrophomonas maltophilia
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.3.28
2'-deoxyadenosine + O2
81.4% of the activity compared to inosine
287612
Stenotrophomonas maltophilia
9-(2-deoxy-beta-D-erythro-pentofuranuronosyl)-9H-purin-6-amine + H2O
-
287612
Stenotrophomonas maltophilia
?
1.1.3.28
adenosine + O2
97.3% of the activity compared to inosine
287612
Stenotrophomonas maltophilia
9-riburonosyladenine + H2O
-
287612
Stenotrophomonas maltophilia
?
1.1.3.28
adenosine + O2
97.3% of the activity compared to inosine
287612
Stenotrophomonas maltophilia LB-86
9-riburonosyladenine + H2O
-
287612
Stenotrophomonas maltophilia LB-86
?
1.1.3.28
cytidine + O2
77.3% of the activity compared to inosine
287612
Stenotrophomonas maltophilia
1-riburonosylcytosine + H2O
-
287612
Stenotrophomonas maltophilia
?
1.1.3.28
cytidine + O2
77.3% of the activity compared to inosine
287612
Stenotrophomonas maltophilia LB-86
1-riburonosylcytosine + H2O
-
287612
Stenotrophomonas maltophilia LB-86
?
1.1.3.28
deoxycytidine + O2
77.3% of the activity compared to inosine
287612
Stenotrophomonas maltophilia
1-riburonosylcytosine + H2O
-
287612
Stenotrophomonas maltophilia
?
1.1.3.28
deoxyguanosine + O2
92.3% of the activity compared to inosine
287612
Stenotrophomonas maltophilia
9-riburonosyldeoxyguanine + H2O
-
287612
Stenotrophomonas maltophilia
?
1.1.3.28
deoxyinosine + O2
80.7% of the activity compared to inosine
287612
Stenotrophomonas maltophilia
9-riburonosyldeoxyhypoxanthine + H2O
-
287612
Stenotrophomonas maltophilia
?
1.1.3.28
guanosine + O2
121% of the activity compared to inosine
287612
Stenotrophomonas maltophilia
9-riburonosylguanine + H2O
-
287612
Stenotrophomonas maltophilia
?
1.1.3.28
guanosine + O2
121% of the activity compared to inosine
287612
Stenotrophomonas maltophilia LB-86
9-riburonosylguanine + H2O
-
287612
Stenotrophomonas maltophilia LB-86
?
1.1.3.28
inosine + O2
oxidation of various nucleosides, but no oxidation of nucleotides, bases and ribose
287612
Stenotrophomonas maltophilia
9-riburonosylhypoxanthine + H2O
-
287612
Stenotrophomonas maltophilia
?
1.1.3.28
thymidine + O2
55.8% of the activity compared to inosine
287612
Stenotrophomonas maltophilia
1-riburonosylthymine + H2O
-
287612
Stenotrophomonas maltophilia
?
1.1.3.28
uridine + O2
-
287612
Stenotrophomonas maltophilia
1-riburonosyluracil + H2O
-
287612
Stenotrophomonas maltophilia
?
1.1.3.28
uridine + O2
-
287612
Stenotrophomonas maltophilia LB-86
1-riburonosyluracil + H2O
-
287612
Stenotrophomonas maltophilia LB-86
?
1.1.3.28
xanthosine + O2
best substrate tested, 125% of the activity compared to inosine
287612
Stenotrophomonas maltophilia
9-riburonosylxanthine + H2O
-
287612
Stenotrophomonas maltophilia
?
1.1.3.28
xanthosine + O2
best substrate tested, 125% of the activity compared to inosine
287612
Stenotrophomonas maltophilia LB-86
9-riburonosylxanthine + H2O
-
287612
Stenotrophomonas maltophilia LB-86
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.3.28
heterotetramer
alpha,beta,gamma,delta 1 * 76000 + 1 * 33000 + 1 * 18000 + 1 * 14000, SDS-PAGE, subunits beta and delta appear to be linked together by disulfide bonds
Stenotrophomonas maltophilia
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.3.28
60
-
stable below, complete loss of activity at 75°C
Stenotrophomonas maltophilia
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.3.28
5
6
-
Stenotrophomonas maltophilia
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.1.3.28
5
6
stable for 1 h at 37°C
Stenotrophomonas maltophilia