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Literature summary extracted from

  • Streitenberger, S.A.; Lopez-Mas, J.A.; Sanchez-Ferrer, A.; Garcia-Carmona, J.A.
    Highly efficient Aerococcus viridians L-alpha-glycerophosphate oxidase production in the presence of H2O2-decomposing agent: purification and kinetic characterization (2001), Appl. Microbiol. Biotechnol., 57, 329-333.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.21 benzoylformic acid strain CETC 978, competitive inhibition Aerococcus viridans
1.1.3.21 glyoxylic acid strain CETC 978, competitive inhibition Aerococcus viridans
1.1.3.21 methylglyoxal strain CETC 978, 277 mM, complete inactivation after 50 min, in the presence of 30 mM benzoylformic acid complete inactivation after 20 min Aerococcus viridans
1.1.3.21 additional information strain CETC 978, not inhibited by 20-80 mM fructose-1-phosphate or fructose-6-phosphate Aerococcus viridans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.21 2.3
-
 sn-glycerol 3-phosphate strain CETC 978 Aerococcus viridans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.21 63000
-
 strain CETC 978, x * 63000, SDS-PAGE Aerococcus viridans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.21 sn-glycerol-3-phosphate + O2 Aerococcus viridans
-
 glycerone phosphate + H2O2 i.e. dihydroxyacetone phosphate ?
1.1.3.21 sn-glycerol-3-phosphate + O2 Aerococcus viridans CETC 978
-
 glycerone phosphate + H2O2 i.e. dihydroxyacetone phosphate ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.21 Aerococcus viridans
-
-
-
1.1.3.21 Aerococcus viridans CETC 978
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.21 strain CETC 978, Triton X-114, ammonium sulfate, ion-exchange chromatography, hydrophobic chromatography Aerococcus viridans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.21 433.2
-
 strain CETC 978 Aerococcus viridans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.21 sn-glycerol 3-phosphate + O2
-
 Aerococcus viridans glycerone phosphate + H2O2
-
 r
1.1.3.21 sn-glycerol 3-phosphate + O2
-
 Aerococcus viridans CETC 978 glycerone phosphate + H2O2
-
 r
1.1.3.21 sn-glycerol-3-phosphate + O2
-
 Aerococcus viridans glycerone phosphate + H2O2 i.e. dihydroxyacetone phosphate ?
1.1.3.21 sn-glycerol-3-phosphate + O2
-
 Aerococcus viridans CETC 978 glycerone phosphate + H2O2 i.e. dihydroxyacetone phosphate ?

Subunits

EC Number Subunits Comment Organism
1.1.3.21 ? strain CETC 978, x * 63000, SDS-PAGE Aerococcus viridans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.21 8
-
 strain CETC 978 Aerococcus viridans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.21 FAD
-
 Aerococcus viridans